Lucie Dlouha1,2, Terezie Pelikanova3, Jiří Veleba3, Vera Adamkova4, Vera Lanska5, Tomas Sosna3, Lukas Pacal6, Katerina Kankova6, Jaroslav A Hubacek7,8. 1. Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague, 14021, Czech Republic. 2. Department of Anthropology and Human Genetics, Faculty of Science, Charles University, Vinicka 7, 12808, Prague, Czech Republic. 3. Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague, 14021, Czech Republic. 4. Department of Preventive Cardiology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague, 14021, Czech Republic. 5. Statistical Unit, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague, 14021, Czech Republic. 6. Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic. 7. Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague, 14021, Czech Republic. jahb@ikem.cz. 8. 3rd Department of Internal Medicine, 1st Faculty of Medicine, Charles University, U Nemocnice 1, 12808, Prague, Czech Republic. jahb@ikem.cz.
Abstract
BACKGROUND: Common polymorphisms within the apolipoprotein E (APOE) gene are suggested to be associated with the development of type 2 diabetes mellitus (T2DM), but the potential association with T2DM complications (nephropathy, neuropathy and retinopathy) remains unclear. We perform the case-control study to analyse the association between the APOE polymorphism and risk of T2DM and to analysed the potential relationship between the APOE and T2DM complications. METHODS AND RESULTS: APOE variants (rs429358 and rs7412) were genotyped by TaqMan assay in T2DM patients (N = 1274; N = 829 with complications including retinopathy, neuropathy and nephropathy status) and with PCR-RFLP in healthy nondiabetic controls (N = 2055). The comparison of subjects with genotypes associated with low plasma cholesterol (APOE2/E2 and APOE2/E3 carriers vs. others) did not show an association with T2DM (OR [95% CI] = 0.88 [0.71-1.08). The differences remained insignificant after adjusting for diabetes duration, sex and BMI. Carriers of at least one APOE4 allele (rs429358) are protected against T2DM related retinopathy (OR [95% CI] = 0.65 [0.42-0.99]. Protection against retinopathy is driven mostly by females (OR [95% CI] = 0.50 [0.25-0.99]); and remains significant (P = 0.044) after adjustment for diabetes duration and BMI. CONCLUSION: Common APOE polymorphism was not associated with T2DM in the Czech population. Yet, APOE4 allele revealed an association with retinopathy. In particular, female T2DM patients with at least one APOE4 allele exhibit lower prevalence of retinopathy in our study subjects.
BACKGROUND: Common polymorphisms within the apolipoprotein E (APOE) gene are suggested to be associated with the development of type 2 diabetes mellitus (T2DM), but the potential association with T2DM complications (nephropathy, neuropathy and retinopathy) remains unclear. We perform the case-control study to analyse the association between the APOE polymorphism and risk of T2DM and to analysed the potential relationship between the APOE and T2DM complications. METHODS AND RESULTS: APOE variants (rs429358 and rs7412) were genotyped by TaqMan assay in T2DM patients (N = 1274; N = 829 with complications including retinopathy, neuropathy and nephropathy status) and with PCR-RFLP in healthy nondiabetic controls (N = 2055). The comparison of subjects with genotypes associated with low plasma cholesterol (APOE2/E2 and APOE2/E3 carriers vs. others) did not show an association with T2DM (OR [95% CI] = 0.88 [0.71-1.08). The differences remained insignificant after adjusting for diabetes duration, sex and BMI. Carriers of at least one APOE4 allele (rs429358) are protected against T2DM related retinopathy (OR [95% CI] = 0.65 [0.42-0.99]. Protection against retinopathy is driven mostly by females (OR [95% CI] = 0.50 [0.25-0.99]); and remains significant (P = 0.044) after adjustment for diabetes duration and BMI. CONCLUSION: Common APOE polymorphism was not associated with T2DM in the Czech population. Yet, APOE4 allele revealed an association with retinopathy. In particular, female T2DM patients with at least one APOE4 allele exhibit lower prevalence of retinopathy in our study subjects.
Authors: Elisenda Climent; Sofía Pérez-Calahorra; Victoria Marco-Benedí; Nuria Plana; Rosa Sánchez; Emilio Ros; Juan F Ascaso; Jose Puzo; Fátima Almagro; Carlos Lahoz; Fernando Civeira; Juan Pedro-Botet Journal: Sci Rep Date: 2017-07-17 Impact factor: 4.379
Authors: Isabelle Romieu; Laure Dossus; Simón Barquera; Hervé M Blottière; Paul W Franks; Marc Gunter; Nahla Hwalla; Stephen D Hursting; Michael Leitzmann; Barrie Margetts; Chizuru Nishida; Nancy Potischman; Jacob Seidell; Magdalena Stepien; Youfa Wang; Klaas Westerterp; Pattanee Winichagoon; Martin Wiseman; Walter C Willett Journal: Cancer Causes Control Date: 2017-02-17 Impact factor: 2.506