Junying Zhao1,2, Wei Yi3, Bin Liu1,2, Yaohua Dai4, Tiemin Jiang1,5, Shuxing Chen6, Jianwu Wang7, Baowen Feng8, Weicang Qiao1,2, Yanpin Liu1,2, Huihuang Zhou9, Jianying He10, Juncai Hou11, Lijun Chen12,13. 1. National Engineering Center of Dairy for Maternal and Child Health, Beijing Sanyuan Foods Co. Ltd., No. 8, Yingchang Street, Yinghai Town, Daxing District, Beijing, 100163, China. 2. Beijing Engineering Research Center of Dairy, Beijing Technical Innovation Center of Human Milk Research, Beijing Sanyuan Foods Co. Ltd., No. 8, Yingchang Street, Yinghai Town, Daxing District, Beijing, 100163, China. 3. Beijing Ditan Hospital Capital Medical University, Beijing, 100015, China. 4. Capital Institute of Pediatrics, Beijing, 100020, China. 5. South Asia Branch of National Engineering Center of Dairy for Maternal and Child Health, Guilin University of Technology, Guilin, 541006, China. 6. Henan University of Science and Technology, Luoyang, 471000, Henan Province, China. 7. XiangYa School of Public Health, Central South University, Changsha, 410078, Hunan Province, China. 8. Tongzhou Maternal and Child Health Hospital of Beijing, Beijing, 101101, China. 9. Maternal and Child Health Hospital of Liuyang of Hunan Province, Changsha, 410300, Hunan Province, China. 10. Luoyang Northern Enterprises Group Hospital, Luoyang, 471000, Henan Province, China. 11. Food Science College of Northeast Agricultural University, Harbin, 150030, China. 12. National Engineering Center of Dairy for Maternal and Child Health, Beijing Sanyuan Foods Co. Ltd., No. 8, Yingchang Street, Yinghai Town, Daxing District, Beijing, 100163, China. chenlijun@sanyuan.com.cn. 13. Beijing Engineering Research Center of Dairy, Beijing Technical Innovation Center of Human Milk Research, Beijing Sanyuan Foods Co. Ltd., No. 8, Yingchang Street, Yinghai Town, Daxing District, Beijing, 100163, China. chenlijun@sanyuan.com.cn.
Abstract
PURPOSE: Infant gut microbiota which plays an important role in long-term health is mainly shaped by early life nutrition. However, the effect of nutrients on infants gut microbiota is less researched. Here, we present a study aiming to investigate in vitro a modified formula that is supplemented with milk fat globule membrane (MFGM) that were missing in common formulas when compared with human milk and to assess the impact of feeding scheme on microbiota and metabolism. METHODS: A total of 44 infants including 16 from breast milk feeding, 13 from common formula feeding and 15 from modified formula feeding were analyzed, and A cross-sectional sampling of fecal and urine was done at 1 month-of-age. Stool microbiota composition was characterized using high-throughput DNA sequencing, and urinary metabolome was profiled by nuclear magnetic resonance (NMR). In vitro growth experiment of Bifidobacterium with key components from MFGM was performed and analyzed by both DNA and RNA. RESULTS: Stool samples from the infants who were breastfed had a higher relative abundance of Bifidobacterium and a lower relative abundance of Escherichia than the formula-fed infants. The stool microbiome shifts were associated with urine metabolites changes. Three substances including lactadherin, sialic acid and phospholipid, key components of MFGM were significantly positively correlated to Bifidobacterium of stool samples from infants, and stimulated the growth rate of Bifidobacterium significantly by provided energy in vitro growth experiment with RNA analysis. CONCLUSIONS: These findings suggest that the key components from MFGM could improve infants' health by modulating the gut microbiome, and possibly supporting the growth of Bifidobacterium. REGISTRATION: Clinicaltrials.gov NCT02658500 (registered on January 20, 2016).
PURPOSE: Infant gut microbiota which plays an important role in long-term health is mainly shaped by early life nutrition. However, the effect of nutrients on infants gut microbiota is less researched. Here, we present a study aiming to investigate in vitro a modified formula that is supplemented with milk fat globule membrane (MFGM) that were missing in common formulas when compared with human milk and to assess the impact of feeding scheme on microbiota and metabolism. METHODS: A total of 44 infants including 16 from breast milk feeding, 13 from common formula feeding and 15 from modified formula feeding were analyzed, and A cross-sectional sampling of fecal and urine was done at 1 month-of-age. Stool microbiota composition was characterized using high-throughput DNA sequencing, and urinary metabolome was profiled by nuclear magnetic resonance (NMR). In vitro growth experiment of Bifidobacterium with key components from MFGM was performed and analyzed by both DNA and RNA. RESULTS: Stool samples from the infants who were breastfed had a higher relative abundance of Bifidobacterium and a lower relative abundance of Escherichia than the formula-fed infants. The stool microbiome shifts were associated with urine metabolites changes. Three substances including lactadherin, sialic acid and phospholipid, key components of MFGM were significantly positively correlated to Bifidobacterium of stool samples from infants, and stimulated the growth rate of Bifidobacterium significantly by provided energy in vitro growth experiment with RNA analysis. CONCLUSIONS: These findings suggest that the key components from MFGM could improve infants' health by modulating the gut microbiome, and possibly supporting the growth of Bifidobacterium. REGISTRATION: Clinicaltrials.gov NCT02658500 (registered on January 20, 2016).
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