Literature DB >> 34321513

A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.

Frederic Strobl1, Ernst H K Stelzer2.   

Abstract

We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
© 2021. The Author(s).

Entities:  

Year:  2021        PMID： 34321513     DOI： 10.1038/s41598-021-94288-0

Source DB:  PubMed          Journal:  Sci Rep        ISSN： 2045-2322            Impact factor:   4.379


  11 in total

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6.  Non-invasive instant genotyping of fluorescently labelled transgenic mice.

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7.  A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines.

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1.  QuickPIV: Efficient 3D particle image velocimetry software applied to quantifying cellular migration during embryogenesis.

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Journal:  BMC Bioinformatics       Date:  2021-12-04       Impact factor: 3.169
  1 in total

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