Evaluation of humic acid conversion during composting under amoxicillin stress: Emphasizes the driving role of core microbial communities.

This study aimed to investigate the effects of various amoxicillin (AMX) concentrations on humic acid (HA) formation and the bacterial community structure (BCS) during chicken manure aerobic composting. The findings showed that AMX caused a rise in pH and a shortening of the thermophilic cycle during the thermophilic period. Moreover, a high concentration of AMX (150 mg/kg) inhibited community succession and humification at maturity. In addition to influencing environmental factors, AMX increased the relative abundance (RA) of the dominant bacteria in the thermophilic phase (Firmicutes) and decreased the RA of the dominant bacteria in the mature phase (Proteobacteria). From carbon conversion, AMX promoted fulvic acid (FA) anabolism in the early period and inhibited HA accumulation in the later period. As a result, the primary impact of AMX on HA transformation was the alteration of the BCS, ultimately affecting the quality of compost.