| Literature DB >> 34319989 |
Lin Wen1,2, Tao Zhang1,2, Jinxuan Wang1,2, Xuepu Jin1,2, Muhammad Abdul Rouf1,2, Desha Luo1,2, Yuan Zhu1,2, Daoxi Lei1,2, Hans Gregersen1,2, Yeqi Wang1,2, Guixue Wang1,2.
Abstract
Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven by endothelial cell (EC) rearrangement, which involves EC nucleus migration, junction remodeling, and actin cytoskeleton remodeling. Further observation reveals a growing difference in blood flow velocity between the two vessels in CV pruning in zebrafish embryos. With this model, we identify the critical role of Kruppel-like factor 6a (klf6a) in CV pruning. Disruption of klf6a functioning impairs CV pruning in zebrafish. klf6a is required for EC nucleus migration, junction remodeling, and actin cytoskeleton dynamics in zebrafish embryos. Moreover, actin-related protein transgelin 2 (tagln2) is a direct downstream target of klf6a in CV pruning in zebrafish embryos. Together these results demonstrate that the klf6a-tagln2 axis regulates CV pruning by promoting EC rearrangement.Entities:
Year: 2021 PMID： 34319989 DOI： 10.1371/journal.pgen.1009690
Source DB: PubMed Journal: PLoS Genet ISSN： 1553-7390 Impact factor: 5.917