Literature DB >> 3430582

Improved specimen support cups and auxiliary devices for the Balzers high pressure freezing apparatus.

S Craig1, J C Gilkey, L A Staehelin.   

Abstract

A new design for interlocking specimen support cups that permits reliable and reproducible freezing under high pressures of a range of different biological specimens is described. A stronger holder in which the support cups are tightly held during pressurization and freezing, and a sliding wedge for separating the cups after freezing are also described. Used in conjunction with the Balzers HPM 010 high pressure freezing apparatus, these modifications permit routine freezing of tissue for freeze-fracture and freeze-substitution for electron microscopy.

Mesh：

Year:  1987        PMID： 3430582     DOI： 10.1111/j.1365-2818.1987.tb02857.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  7 in total

1.  Comparison of the ultrastructure of conventionally fixed and high pressure frozen/freeze substituted root tips of Nicotiana and Arabidopsis.

Authors:  J Z Kiss; T H Giddings; L A Staehelin; F D Sack
Journal:  Protoplasma       Date:  1990       Impact factor: 3.356

2.  Slr2013 is a novel protein regulating functional assembly of photosystem II in Synechocystis sp. strain PCC 6803.

Authors:  Galyna I Kufryk; Wim F J Vermaas
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

Review 3.  Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material.

Authors:  Kent L McDonald
Journal:  Protoplasma       Date:  2013-11-21       Impact factor: 3.356

Review 4.  Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution.

Authors:  Daniel Studer; Bruno M Humbel; Matthias Chiquet
Journal:  Histochem Cell Biol       Date:  2008-09-16       Impact factor: 4.304

5.  Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples.

Authors:  L A Staehelin; T H Giddings; J Z Kiss; F D Sack
Journal:  Protoplasma       Date:  1990       Impact factor: 3.356

6.  Increased Expression of Vacuolar Aquaporin and H+-ATPase Related to Motor Cell Function in Mimosa pudica L.

Authors:  P. Fleurat-Lessard; N. Frangne; M. Maeshima; R. Ratajczak; J. L. Bonnemain; E. Martinoia
Journal:  Plant Physiol       Date:  1997-07       Impact factor: 8.340

7.  Exocytosis in normal anterior pituitary cells. Quantitative correlation between growth hormone release and the morphological features of exocytosis.

Authors:  B Draznin; R Dahl; N Sherman; K E Sussman; L A Staehelin
Journal:  J Clin Invest       Date:  1988-04       Impact factor: 14.808
  7 in total

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