Literature DB >> 3430579

A temperature controlled chamber to allow observation and measurement of uptake of fluorochromes into live cells.

J N Payne1, J D Cooper, S T MacKeown, R W Horobin.   

Abstract

A temperature controlled cell chamber is described which permits phase-contrast and fluorescence microscopy of living cells. It is shown how the cellular uptake of fluorochromes can be investigated both photographically and photometrically. Some sample results are presented for the uptake of Evans Blue.

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Year:  1987        PMID： 3430579     DOI： 10.1111/j.1365-2818.1987.tb02844.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  4 in total

1.  A new perfusion cell chamber system for determination of heat shock effects by means of video-enhanced microscopy.

Authors:  A Hofer; F Nagel; F Wonka; H E Krinke; F Gölfert; R H Funk
Journal:  Med Biol Eng Comput       Date:  1999-09       Impact factor: 2.602

2.  Interaction of molecular probes with living cells and tissues. Part 2. A structure-activity analysis of mitochondrial staining by cationic probes, and a discussion of the synergistic nature of image-based and biochemical approaches.

Authors:  F Rashid; R W Horobin
Journal:  Histochemistry       Date:  1990

3.  Fluorescent cationic probes for nuclei of living cells: why are they selective? A quantitative structure-activity relations analysis.

Authors:  Richard W Horobin; Juan C Stockert; Fiza Rashid-Doubell
Journal:  Histochem Cell Biol       Date:  2006-02-07       Impact factor: 4.304

4.  Predicting the behaviour and selectivity of fluorescent probes for lysosomes and related structures by means of structure-activity models.

Authors:  F Rashid; R W Horobin; M A Williams
Journal:  Histochem J       Date:  1991-10
  4 in total

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