Differential effects of acetylcholine in the chicken auditory neostriatum and hyperstriatum ventrale--studies in vivo and in vitro.

1. The effect of acetylcholine (ACh) on the response properties of single units in the caudal auditory telencephalon was studied both in awake chickens and in an in vitro slice preparation. 2. Two types of electrophysiological behavior in response to ACh were observed: an inhibition of cell firing typical for the majority of neurons in the auditory hyperstriatum ventrale and a facilitation of neuronal responses seen predominantly in neostriatal auditory units. 3. The facilitatory effect of ACh is also present in hyperstriatal cells, but is usually dominated by an indirect inhibition. 4. ACh-induced facilitation on single unit responses could be mimicked in awake birds by applying potentially arousing sensory stimuli. 5. The effects of ACh are antagonized by the muscarinic receptor blocker scopolamine. 6. Inhibitory responses can also be antagonized by the GABA-antagonist bicuculline and thus can be attributed to an ACh-induced activation of GABAergic inhibitory interneurons. Evidence is given that the facilitatory responses result from a closure of voltage-dependent potassium channels. 7. The results are discussed with respect to a possible role of cholinergic afferents in telencephalic processing of auditory information and in comparison with the cholinergic influences in the mammalian neocortex.