Bioengineering for curcumin loaded carboxymethyl guargum/reduced graphene oxide nanocomposites for chronic wound healing applications.

Biomimetic scaffolds engineering for improved collagen, epithelial cutaneous and fibrous tissue regeneration remains challenging for wound healing. To address these issues, this study aimed to report on the fabrication and characterization of electrospun of carboxymethyl guargum (CMGG), reduced graphene oxide (rGO) nanocomposite dressings loaded with curcumin for chronic wound healing applications. SEM and XRD examined the morphology of nanofibers and resulted in excellent porosity. TGA and FT-IR were done, which revealed the nanofibers' thermal and chemical interactions. CMGG, rGO nanocomposite with curcumin was investigated for in-vitro wound healing assay by scratch wound healing model using 3T3 L1 fibroblast cell lines and conducted in vitro drug-releasing studies. These nanocomposites showed 100% wound closure by the proliferation of fibroblast cell lines 3T3-L1 within 48 h and showed controlled drug release. Further, in vivo results also showed that the CMGG, rGO nanocomposite with curcumin has the potential wound healing effects. Histological studies showed that the CMGG, rGO nanocomposite with curcumin has the potential for wound healing, which indicates that the biomimetic CMGG nanofibers have an excellent healing effect on chronic wounds.