Literature DB >> 34302655

Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells.

Misato Nakanishi-Koakutsu1,2, Tadashi Takaki1,2,3, Kenji Miki1,2, Yoshinori Yoshida4.   

Abstract

Human iPSC-derived cardiomyocytes (hiPSC-CMs) are expected to be used in regenerative therapies and drug discovery for heart failure. hiPSC-CMs are a mixture of mainly ventricular CMs (VCMs) and also of atrial CMs (ACMs) and pacemaker cells. Here we describe a method to enrich VCM and ACM differentiation and to characterize these subtypes by gene expression analysis using qRT-PCR and by electrophysiological properties using the patch-clamp method. The differentiated VCMs and ACMs highly express VCM and ACM marker genes, respectively. Furthermore, both subtypes show specific properties of action potentials.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Action potential; Atrial cardiomyocytes; Flow cytometry; Patch-clamp; Subtype gene expression; Ventricular cardiomyocytes; hiPSC-derived cardiomyocytes

Year:  2021        PMID： 34302655     DOI： 10.1007/978-1-0716-1484-6_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  Subtype-specific promoter-driven action potential imaging for precise disease modelling and drug testing in hiPSC-derived cardiomyocytes.

Authors:  Zhifen Chen; Wenying Xian; Milena Bellin; Tatjana Dorn; Qinghai Tian; Alexander Goedel; Lisa Dreizehnter; Christine M Schneider; Dorien Ward-van Oostwaard; Judy King Man Ng; Rabea Hinkel; Luna Simona Pane; Christine L Mummery; Peter Lipp; Alessandra Moretti; Karl-Ludwig Laugwitz; Daniel Sinnecker
Journal:  Eur Heart J       Date:  2017-01-21       Impact factor: 29.983
  1 in total

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