Literature DB >> 34302646

Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches.

Kenji Miki1, Hirohide Saito2, Yoshinori Yoshida3.   

Abstract

The most common method for isolating cells of interest is an antibody method that recognizes cell surface antigens. However, specific surface antigens for many cell types have not been identified. We have developed the microRNA (miRNA)-responsive synthetic mRNA systems (miRNA switches), which isolate target cells based on endogenous miRNA activity. In this chapter, we describe protocols for isolating human pluripotent stem cell (hPSC)-derived cardiomyocytes using miRNA switches with or without cell sorting.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cardiomyocytes; Human pluripotent stem cells; Isolation; Synthetic modified mRNA; miRNA switch

Year:  2021        PMID： 34302646     DOI： 10.1007/978-1-0716-1484-6_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  Subtype-specific promoter-driven action potential imaging for precise disease modelling and drug testing in hiPSC-derived cardiomyocytes.

Authors:  Zhifen Chen; Wenying Xian; Milena Bellin; Tatjana Dorn; Qinghai Tian; Alexander Goedel; Lisa Dreizehnter; Christine M Schneider; Dorien Ward-van Oostwaard; Judy King Man Ng; Rabea Hinkel; Luna Simona Pane; Christine L Mummery; Peter Lipp; Alessandra Moretti; Karl-Ludwig Laugwitz; Daniel Sinnecker
Journal:  Eur Heart J       Date:  2017-01-21       Impact factor: 29.983
  1 in total

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