Sandra Pritzkow1, Damian Gorski1, Frank Ramirez1, Glenn C Telling2, Sylvie L Benestad3, Claudio Soto1. 1. Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas McGovern Medical School at Houston, Houston, Texas, USA. 2. Prion Research Center, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA. 3. Norwegian Veterinary Institute, OIE Reference Laboratory for CWD, Ås, Norway.
Abstract
BACKGROUND: Chronic wasting disease (CWD) is a rapidly spreading prion disorder affecting various species of wild and captive cervids. The risk that CWD poses to cohabiting animals or more importantly to humans is largely unknown. METHODS: In this study, we investigated differences in the capacity of CWD isolates obtained from 6 different cervid species to induce prion conversion in vitro by protein misfolding cyclic amplification. We define and quantify spillover and zoonotic potential indices as the efficiency by which CWD prions sustain prion generation in vitro at expenses of normal prion proteins from various mammals and human, respectively. RESULTS: Our data suggest that reindeer and red deer from Norway could be the most transmissible CWD prions to other mammals, whereas North American CWD prions were more prone to generate human prions in vitro. CONCLUSIONS: Our results suggest that Norway and North American CWD prions correspond to different strains with distinct spillover and zoonotic potentials.
BACKGROUND: Chronic wasting disease (CWD) is a rapidly spreading prion disorder affecting various species of wild and captive cervids. The risk that CWD poses to cohabiting animals or more importantly to humans is largely unknown. METHODS: In this study, we investigated differences in the capacity of CWD isolates obtained from 6 different cervid species to induce prion conversion in vitro by protein misfolding cyclic amplification. We define and quantify spillover and zoonotic potential indices as the efficiency by which CWD prions sustain prion generation in vitro at expenses of normal prion proteins from various mammals and human, respectively. RESULTS: Our data suggest that reindeer and red deer from Norway could be the most transmissible CWD prions to other mammals, whereas North American CWD prions were more prone to generate human prions in vitro. CONCLUSIONS: Our results suggest that Norway and North American CWD prions correspond to different strains with distinct spillover and zoonotic potentials.
Authors: P Parchi; A Giese; S Capellari; P Brown; W Schulz-Schaeffer; O Windl; I Zerr; H Budka; N Kopp; P Piccardo; S Poser; A Rojiani; N Streichemberger; J Julien; C Vital; B Ghetti; P Gambetti; H Kretzschmar Journal: Ann Neurol Date: 1999-08 Impact factor: 10.422
Authors: Jifeng Bian; Jeffrey R Christiansen; Julie A Moreno; Sarah J Kane; Vadim Khaychuk; Joseph Gallegos; Sehun Kim; Glenn C Telling Journal: Proc Natl Acad Sci U S A Date: 2019-05-30 Impact factor: 11.205
Authors: Juan Carlos Espinosa; Alba Marín-Moreno; Patricia Aguilar-Calvo; Sylvie L Benestad; Olivier Andreoletti; Juan María Torres Journal: J Infect Dis Date: 2021-03-29 Impact factor: 5.226
Authors: Jonathan D F Wadsworth; Susan Joiner; Jacqueline M Linehan; Kezia Jack; Huda Al-Doujaily; Helena Costa; Thea Ingold; Maged Taema; Fuquan Zhang; Malin K Sandberg; Sebastian Brandner; Linh Tran; Turid Vikøren; Jørn Våge; Knut Madslien; Bjørnar Ytrehus; Sylvie L Benestad; Emmanuel A Asante; John Collinge Journal: J Infect Dis Date: 2022-09-13 Impact factor: 7.759