Yanchuan Li1, Xiaohua Yuan2, Ziyun Shi1, Haili Wang1, Duomei Ren1, Ya Zhang1, Yangyang Fan1, Yanfeng Liu3, Zhangxia Cui4. 1. Department of Obstetrics, Shaanxi Provincial People's Hospital, Xi'an, China. 2. Department of Obstetrics and Gynecology, Shaanxi Provincial People's Hospital, Xi'an, China. 3. Department of General Surgery, The Second Affiliated Hospital, Xi'an Medical University, Xi'an, China. 4. Department of Obstetrics, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine (Xi Xian Central Hospital), Xianyang, China.
Abstract
BACKGROUND: Gestational diabetes mellitus (GDM) is increasingly common in pregnancy. This study's purpose was to identify the expression of XIST and manifest the potential mechanism of XIST in GDM. METHODS: Ninety-three patients with GDM and 93 normal pregnant women were included in this investigation. qRT-PCR was conducted to evaluate the expression of miR-497-5p and XIST and the relationship between XIST and fasting blood glucose (FBG) was explored by Pearson assay. The clinical diagnosis of XIST on GDM patients was validated by the receiver operator characteristic (ROC) curve. Cell counting kit-8 (CCK-8) was applied to elucidate cell viability. Luciferase reporter assay was performed to document the relationship among XIST, miR-497-5p, and FOXO1. RESULTS: The expression of XIST was increased in GDM patients and HTR-8/SVneo cell models caused by high glucose (HG). The expression of XIST was associated with the FBG levels and appeared to be a feasible indicator in discriminating GDM patients. The expression of miR-497-5p was prominently reduced in GDM patients and cell models. Inhibition of XIST might alleviate the adverse function of HG on cell viability via sponging miR-497-5p. FOXO1 was proved to be a downstream target gene of miR-497-5p. CONCLUSIONS: Overexpression of XIST and downregulation of miR-497-5p were indicated in this publication. XIST might serve as a promising diagnostic marker for GDM patients. XIST/miR-497-5p/FOXO1 axis played a critical role in the regulation of trophoblast cells. 2021 Cardiovascular Diagnosis and Therapy. All rights reserved.
BACKGROUND: Gestational diabetes mellitus (GDM) is increasingly common in pregnancy. This study's purpose was to identify the expression of XIST and manifest the potential mechanism of XIST in GDM. METHODS: Ninety-three patients with GDM and 93 normal pregnant women were included in this investigation. qRT-PCR was conducted to evaluate the expression of miR-497-5p and XIST and the relationship between XIST and fasting blood glucose (FBG) was explored by Pearson assay. The clinical diagnosis of XIST on GDM patients was validated by the receiver operator characteristic (ROC) curve. Cell counting kit-8 (CCK-8) was applied to elucidate cell viability. Luciferase reporter assay was performed to document the relationship among XIST, miR-497-5p, and FOXO1. RESULTS: The expression of XIST was increased in GDM patients and HTR-8/SVneo cell models caused by high glucose (HG). The expression of XIST was associated with the FBG levels and appeared to be a feasible indicator in discriminating GDM patients. The expression of miR-497-5p was prominently reduced in GDM patients and cell models. Inhibition of XIST might alleviate the adverse function of HG on cell viability via sponging miR-497-5p. FOXO1 was proved to be a downstream target gene of miR-497-5p. CONCLUSIONS: Overexpression of XIST and downregulation of miR-497-5p were indicated in this publication. XIST might serve as a promising diagnostic marker for GDM patients. XIST/miR-497-5p/FOXO1 axis played a critical role in the regulation of trophoblast cells. 2021 Cardiovascular Diagnosis and Therapy. All rights reserved.
Authors: Kathleen A Page; Shan Luo; Xinhui Wang; Ting Chow; Jasmin Alves; Thomas A Buchanan; Anny H Xiang Journal: Diabetes Care Date: 2019-05-21 Impact factor: 19.112
Authors: Anny H Xiang; Xinhui Wang; Mayra P Martinez; Darios Getahun; Kathleen A Page; Thomas A Buchanan; Klara Feldman Journal: Diabetes Care Date: 2018-10-29 Impact factor: 19.112