Extraction of Au(III) by Microbially Reduced Metal-Organic Frameworks.

Gold is a critical resource in the jewelry and electronics industries and is facing increased consumer demand. Accordingly, methods for its extraction from waste effluents and environmental water sources have been sought to supplement existing mining infrastructure. Redox-mediated treatments, such as Fe(II)-based platforms, offer promise for precipitating soluble Au(III). We hypothesized that microbial generation of Fe(II) in the presence of sorbent metal-organic frameworks could capitalize on the advantages of both biological- and chemical-driven extraction approaches. Toward this aim, we tested Au(III) removal by Shewanella oneidensis cultured with Fe(III)-based materials (ferrihydrite, Fe-BTC, MIL-100, or MIL-127). Across all tested materials, S. oneidensis generated the highest levels of redox-active Fe(II) (1.99 ± 0.27 mM) when cultured with MIL-127 as a respiratory substrate in a bicarbonate-buffered medium. This translated into superior Au(III) removal performance in terms of both removal rate and capacity (k = 2.55 ± 0.60 h-1; Q = 183 mg g-1). Unlike other materials tested, MIL-127 also maintained cell viability following repeated Au(III) challenges, enabling the regeneration of Fe(II) in the framework. Together, these effects facilitated the treatment of multiple cycles of Au(III) by S. oneidensis-reduced MIL-127. Overall, this work demonstrates that microbial generation of Fe(II) can facilitate the removal of Au(III), augmenting purely adsorptive platforms. Given the biological and chemical modularity of our system, our results suggest that future optimizations to microbial Fe(II) generation may offer promise for improving Au(III) extraction processes.