Literature DB >> 34292557

Methods to Screen for Radical SAM Enzyme Crystallization Conditions.

Lydie Martin1, Xavier Vernède1, Yvain Nicolet2.   

Abstract

Radical S-adenosyl-L-methionine proteins most probably belong to the widest superfamily of metalloenzymes. Thanks to their ability to catalyze difficult reactions, combined with their involvement in the biosynthesis of numbers of natural products, they sound promising for various biotechnological applications. Their structural study is often limited because they are usually challenging to crystallize. This chapter presents protocols and equipment developed to quickly screen for crystallization conditions under anaerobic conditions, as exemplified by our recent study of the nitrogenase maturase NifB.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Anaerobic crystallization; Crystal flash cooling. FeMo coassembly; Fe-S cluster reconstitution; FeMo cofactor; Metalloprotein; Protein expression and purification

Mesh：

Substances：

Year:  2021        PMID： 34292557     DOI： 10.1007/978-1-0716-1605-5_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Structural Insights into the Mechanism of the Radical SAM Carbide Synthase NifB, a Key Nitrogenase Cofactor Maturating Enzyme.

Authors:  Ana Sosa Fajardo; Pierre Legrand; Lucı A Payá-Tormo; Lydie Martin; Maria Teresa Pellicer Martı Nez; Carlos Echavarri-Erasun; Xavier Vernède; Luis M Rubio; Yvain Nicolet
Journal:  J Am Chem Soc       Date:  2020-06-11       Impact factor: 15.419

Review 2.  Radical S-adenosylmethionine enzymes.

Authors:  Joan B Broderick; Benjamin R Duffus; Kaitlin S Duschene; Eric M Shepard
Journal:  Chem Rev       Date:  2014-01-29       Impact factor: 60.622

3.  The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters.

Authors:  Tristan Wagner; Ulrich Ermler; Seigo Shima
Journal:  Science       Date:  2016-10-07       Impact factor: 47.728

4.  Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction.

Authors:  Tristan Wagner; Jürgen Koch; Ulrich Ermler; Seigo Shima
Journal:  Science       Date:  2017-08-18       Impact factor: 47.728

Review 5.  Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity.

Authors:  Béatrice Roche; Laurent Aussel; Benjamin Ezraty; Pierre Mandin; Béatrice Py; Frédéric Barras
Journal:  Biochim Biophys Acta       Date:  2013-01-06

Review 6.  Structural insights into radical generation by the radical SAM superfamily.

Authors:  Jessica L Vey; Catherine L Drennan
Journal:  Chem Rev       Date:  2011-03-03       Impact factor: 60.622

7.  AdoMet radical proteins--from structure to evolution--alignment of divergent protein sequences reveals strong secondary structure element conservation.

Authors:  Yvain Nicolet; Catherine L Drennan
Journal:  Nucleic Acids Res       Date:  2004-08-02       Impact factor: 16.971

8.  Crystal structure of tryptophan lyase (NosL): evidence for radical formation at the amino group of tryptophan.

Authors:  Yvain Nicolet; Laura Zeppieri; Patricia Amara; Juan C Fontecilla-Camps
Journal:  Angew Chem Int Ed Engl       Date:  2014-09-05       Impact factor: 15.336

Review 9.  Radical SAM Enzymes in the Biosynthesis of Ribosomally Synthesized and Post-translationally Modified Peptides (RiPPs).

Authors:  Alhosna Benjdia; Clémence Balty; Olivier Berteau
Journal:  Front Chem       Date:  2017-11-08       Impact factor: 5.221

10.  The Structure-Function Linkage Database.

Authors:  Eyal Akiva; Shoshana Brown; Daniel E Almonacid; Alan E Barber; Ashley F Custer; Michael A Hicks; Conrad C Huang; Florian Lauck; Susan T Mashiyama; Elaine C Meng; David Mischel; John H Morris; Sunil Ojha; Alexandra M Schnoes; Doug Stryke; Jeffrey M Yunes; Thomas E Ferrin; Gemma L Holliday; Patricia C Babbitt
Journal:  Nucleic Acids Res       Date:  2013-11-23       Impact factor: 16.971
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  1 in total

1.  Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy.

Authors:  Mickaël V Cherrier; Xavier Vernède; Daphna Fenel; Lydie Martin; Benoit Arragain; Emmanuelle Neumann; Juan C Fontecilla-Camps; Guy Schoehn; Yvain Nicolet
Journal:  Biomolecules       Date:  2022-03-12
  1 in total

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