Uterine Artery Embolization with Small-Sized Particles for the Treatment of Symptomatic Adenomyosis: A 42-Month Clinical Follow-Up.

PURPOSE: To assess the long-term outcome of performing uterine artery embolization (UAE) using small particles in women with symptomatic adenomyosis (AD).
METHODS: Twenty-seven consecutive women (median age 42 years, range 29-53 years) with AD, in eight cases AD combined with fibroids, who underwent UAE between February 2015 and January 2019, were retrospectively analyzed. The embolization was performed using small-sized polyvinyl alcohol particles (100 μm and 300 μm). The patients completed the Uterine Fibroid Symptom and Quality of Life questionnaire at baseline and at a 42-month follow-up (range 24-71). The junction zone (JZ) thickness and uterine volume were also calculated at baseline and at a three-month follow-up.
RESULTS: The total symptom severity score (SSS) decreased from a median of 59 (range 34-78) at baseline to a median of 9 (range 3-47) at the end of this study; the health-related quality of life score (HRQOL) increased from a median of 38 (range 23-49) at baseline to a median of 84 (range 46-97) at 42 months. Twenty of the 27 patients were asymptomatic. The clinical response of the remaining seven women was little improvement in their symptoms, and one of the seven women underwent a hysterectomy at 35 months. Twenty-six of the 27 (96%) patients had a preserved uterus at the 42-month follow-up. There was no difference after UAE in SSS, HRQOL, junction zone (JZ) thickness, and uterus volume between patients with pure AD and those with AD combined with fibroids (p = 0.729, 0.710, 0.973, and > 0.99). There was no difference in the JZ thickness and uterus volume at baseline between the asymptomatic women and the women with an insufficient response (p = 0.854 and 0.253), and there were no major complications afterwards.
CONCLUSION: From the long-term follow-ups, it could be seen that UAE using small particles is safe and effective in treating AD, especially in preserving the uterus. There is no relationship between the clinical outcomes and the initial presence of AD, with or without fibroids, and the JZ thickness at baseline does not seem to be a predictor for the long-term outcome of UAE.

Introduction

Adenomyosis (AD) is a unique pathophysiological condition that sees the formation of ectopic endometrial glands and stroma within the myometrium. It is common in women of reproductive age, and typical symptoms include menorrhagia, chronic pelvic pain, and dysmenorrhea.1–3 Current treatment options for symptomatic AD include hysterectomy, medication, conservative surgery, and minimally invasive techniques, including uterine artery embolization (UAE). However, to date, hysterectomy remains the definitive treatment.4

In recent years, several minimally invasive treatment options for AD have been introduced, and UAE has gained ground as an option for AD, and it is now the most likely alternative to hysterectomy, especially in women with diffuse AD.5,6 At present, there are a variety of embolization materials selected for UAE in the treatment of AD. The embolization materials include polyvinyl alcohol (PVA) particles, tris-acryl gelatin microspheres (TGM), sodium alginate microspheres (KMG), and gelatin sponge/foam particles (GFP), and the primary sizes used are 355–500 μm, 500–700 μm, and 700–900 μm.7 However, it is not clear which particular embolization material can result in better clinical outcomes.

This study analyzed the long-term clinical results of 27 women with AD, eight of whom also had fibroids, who were embolized with small-sized PVA particles.

Materials and Methods

Patients

The Institutional Review Board of the Chinese People’s Liberation Army General Hospital, Beijing, China approved this retrospective study, which was conducted in accordance with the Declaration of Helsinki. Between February 2015 and January 2019, 27 women with a median age of 42 years (range 29–53 years) were evaluated, after presenting with pelvic pain, heavy menstrual bleeding, or bulk-related symptoms, or a combination of them. The clinical diagnosis was confirmed with transvaginal ultrasound or magnetic resonance imaging. All the patients were asked to complete the standardized symptom severity and health-related quality of life questionnaire (The Uterine Fibroid Symptom and Quality of Life [UFS-QoL] questionnaire) and rate the severity of their pelvic pain on the Numerical Rating Scale (NRS) before and after the UAE. Of the 27 women, 19 had pure AD, and eight had AD combined with fibroids, while 25 had diffuse AD, and two had focal AD.

The inclusion criteria for UAE were women with adenomyosis, with or without fibroids, suffering from heavy menstrual bleeding, dysmenorrhea, and/or cycle-independent pain and bulk-related symptoms. These women had had or were receiving medical treatment which did not appear to be working, or they had decided that such treatment was not an option.4

The exclusion criteria for UAE were the suspicion or presence of a malignancy or pelvic infection, an on-going pregnancy or a desire to conceive in the future, absolute contraindication for angiography, deep infiltrating endometriosis requiring surgery or indicating a risk of intestinal stenosis, concurrent hysteroscopic removable submucous fibroids, and being under 18 years of age.4

UAE Embolization

All the patients signed informed consent before the embolization. Patient consent was obtained to review their medical records. The procedures were performed under local anesthesia using the right femoral artery puncture approach. Selective digital subtraction angiography was performed with a 4F Simmons I catheter (Cordis, East Bridgewater, NJ, USA) to evaluate the hypogastric and uterine arteries. Coaxial 2.7-Fr catheters (Progreat, Terumo Corp., Tokyo, Japan) were advanced distally into the uterine artery. Selective uterine artery angiography was performed before the embolization procedure in neutral (6–8 mL contrast medium at 1.5–2 mL/s) to ensure that the tip of the microcatheter was inside or at the ostium of the uterine arteries.

Embolization was performed through the microcatheter using non-spherical PVA particles. PVA particles (Cook Incorporated, Bloomington, IN, USA) (1 mL) were diluted in a 30 mL solution of a non-ionic contrast medium (Visipaque iodixanol 320mg I/mL, GE Healthcare). In all patients, 100 μm particles were used initially, followed by 300 μm particles. The endpoint of the embolization was complete stasis of the blood flow in the uterine artery. After the UAE, angiography of the internal iliac artery was performed to check if other blood vessels were supplying the uterine artery, using the 4F catheter. Embolization was then performed on the contralateral side using the same technique. Ovarian artery angiography was required in patients with a hypoplastic or aplastic uterine artery functionally replaced by an ovarian artery. However, only patients with an ovarian artery entering the myometrium, seen in aortography after the UAE, could be considered for ovarian artery embolization (OAE).

Post-Procedural Management

All the patients stayed in the hospital for 5–7 days for observation. During and after the UAE procedure, intravenous narcotics were administered using a patient-controlled analgesia pump, as well as antiemetic and nonsteroidal anti-inflammatory drugs, for pain control and to reduce symptoms such as nausea and vomiting. Appropriate hydration was given for three days after the UAE, and antibiotics were given for one week to prevent infection.

MRI at Baseline and Follow-Up

MRI was performed before the embolization and at the three-month follow-up. The reduction of the JZ thickness and uterine volume were measured. Uterine volume was obtained by measuring length, width, and height and calculating the volume with the prolapse ellipse equation of vol = (length × width × height) × 0.52.

Technical Assessment

Technical success was defined as the achievement of a unilateral or bilateral uterine arterial catheterization and a successful embolization.

Clinical Assessment

AD-related symptoms were evaluated in all the women before and after the UAE using the Uterine Fibroid Symptom and Quality of Life (UFS-QoL) questionnaire, which produces a symptom severity score (SSS) and a health-related quality of life (HRQOL) score. It consists of an eight-item symptom severity scale and 29 HRQOL items. The eight items of the symptom severity scale are heavy bleeding, passing blood clots, fluctuation in the duration of the menstrual period, fluctuation in the length of the monthly cycle compared to previous cycles, a feeling of tightness or pressure in the pelvic area, frequent urination during the daytime hours, frequent nighttime urination, and feeling fatigued. The 29 HRQOL items comprise six domains: concern, activities, energy/mood, control, self-consciousness, and sexual function. Items are scored on a 5-point Likert scale, ranging from “not at all” to “a very great deal” for symptom severity items and “none of the time” to “all of the time” for the HRQOL items.8 A higher SSS indicates worse symptoms, while a higher HRQOL subscale score indicates a better quality of life. Women with an SSS <20 in combination with an HRQOL score >80 were considered asymptomatic.9 If a patient had an additional UAE or a hysterectomy during follow-up, the timing was recorded.

The NRS for pain is a single 11‐point numeric scale, with 0 representing one pain extreme (eg, “no pain”) and 10 representing the other pain extreme (eg, “pain as bad as you can imagine” or “worst pain imaginable”).

Postembolization symptoms and complications were registered and classified according to the quality improvement guidelines for percutaneous transcatheter embolization.10

Statistical Analysis

Summary descriptive statistics were used for demographic parameters. SSS, HRQOL, JZ thickness, and uterine volume were compared in patients with pure AD and AD with fibroids. The JZ thickness and uterine volume were compared between the asymptomatic patients and the insufficient response patients in a three-month follow-up MRI. A p-value <0.05 was considered statistically significant. Statistical analysis was performed using SPSS 23.0 software for Windows (SPSS Inc., Chicago, Illinois, USA).

Results

Technical Results

Twenty-six (96%) of the patients underwent bilateral UAE, and one underwent unilateral UAE due to the right uterine artery being absent. Only one patient with an ovarian artery supplying the AD had an OAE performed.

Clinical Results

Different scales of abdominal pain were observed in 25 of the patients (92.6%), but these symptoms were relieved during the first week after the procedure, and eight patients presented with a foul vaginal discharge. One patient was suspected of having endometritis and needed broad-spectrum antibiotics. Transient amenorrhea occurred in four women, but no major complications occurred.

The NRS pain score decreased from a median of 8 (5–10) at baseline to a median of 2 (0–5) at the 42-month follow-up. There was a significant difference in the NRS before and after the treatment (p < 0.05).

During a median clinical follow-up of 42 months (range 24–71), 26 of 27 patients had a preserved uterus, but one patient underwent a hysterectomy because the UAE had not improved their condition completely at 35 months. The total SSS decreased from a median of 59 (range 34–78) at baseline to a median of 9 (range 3–47) at 42 months; the HRQOL score increased from a median of 38 (range 23–49) at baseline to a median of 84 (range 46–97) at 42 months. The SSS and HRQOL scores for patients with pure adenomyosis and adenomyosis with fibroids are presented in Table 1. The SSS and the HRQOL scores show no statistical differences between the groups before and after UAE (p = 0.873, 0.729, 0.365, and 0.710) (see Table 1).

Table 1

Clinical Follow-Up at Baseline and 42 Months After Uterine Artery Embolization in Women with Symptomatic Adenomyosis with or without Fibroids

Parameters	Women with Adenomyosis (n=19)	Women with Adenomyosis and Concurrent Fibroids (n=8)	P value
SSS
Baseline	59 (34, 78)	55 (38, 69)	0.873
42-month follow-up	9 (3, 47)	12 (4, 38)	0.729
Reduction	47	45	0.790
HRQOL
Baseline	38 (23, 49)	32 (23, 47)	0.365
42-month follow-up	84 (46, 97)	85 (47–94)	0.710
Increase	48	50	0.873

Twenty out of the 27 women had SSS < 20 combined with an overall HRQOL score >80, indicating they were asymptomatic. The clinical response of the remaining seven women was little improvement in their symptoms. One of these seven women underwent a hysterectomy at 35 months. There was a significant difference in the SSS and HRQOL scores between the asymptomatic group and the insufficient response group after UAE (p < 0.001 for all) (see Table 2).

Table 2

Clinical Follow-Up at Baseline and 42 Months After Uterine Artery Embolization Between Asymptomatic Women and Women with Insufficient Clinical Response

Parameters	Asymptomatic Women (n=20)	Women with Insufficient Clinical Response (n=7)	P value
SSS
Baseline	59 (34, 75)	53 (38, 78)	0.541
42-month follow-up	8 (3, 19)	31 (22, 47)	0.000
Reduction	52	21	0.001
HRQOL
Baseline	34 (23, 49)	41 (23, 47)	0.359
42-month follow-up	89 (80, 97)	49 (46, 68)	0.000
Increase	55	16	0.000

MRI Results

Twenty-two patients had an MRI three months after the UAE. Of these 22 women, 14 women had pure AD, and eight women had AD combined with fibroids. The JZ thickness of these 22 patients at baseline was a median of 33 mm (range 15–61 mm) and at three months a median of 24 mm (range 12–40 mm). There was no difference in JZ thickness between the patients with pure AD or AD combined with fibroids before and after UAE (p = 0.732 and 0.973) (see Table 3).

Table 3

Magnetic Resonance Imaging Follow-Up at Baseline and Three Months After Uterine Artery Embolization in Women with Symptomatic Adenomyosis with or without Fibroids

Parameters	Women with Adenomyosis (n=14)	Women with Adenomyosis and Concurrent Fibroids (n=8)	P value
JZ thickness (mm)
Baseline	39 (15, 61)	32 (26, 49)	0.732
3-month follow-up	26 (12, 40)	23 (16, 34)	0.973
Reduction	12	11	0.373
Uterus volume (cm3)
Baseline	325 (76, 727)	236 (148, 611)	0.838
3-month follow-up	156 (58, 346)	147 (76, 235)	1.000
Reduction	146	110	0.633

The uterus volume of these 22 patients at baseline was a median of 304 cm3 (range 76–726 cm3) and at three months a median of 151 cm3 (range 58–346 cm3). Uterus volume reduction was 151 cm3 (48.3%) (see Figure 1). There was no difference in uterus volume between the patients with pure AD or AD combined with fibroids (p = 0.838 and > 0.99) (see Table 3).

Figure 1

A 43-year-old woman who underwent uterine artery embolization for symptomatic adenomyosis. (A) Sagittal T2-weighted magnetic resonance imaging obtained before uterine artery embolization shows diffuse junctional zone thickening of the uterus, especially in the anterior wall. The volume of the uterus was 608 cm3. (B) Sagittal T2-weighted magnetic resonance imaging obtained three months after uterine artery embolization shows the decreased size of the adenomyosis and the uterus. The volume of the uterus was 212 cm3. (C) Sagittal contrast-enhanced T1-weighted magnetic resonance imaging obtained three months after uterine artery embolization shows that the adenomyosis was almost completely necrotic.

Of these 22 women, 16 women were asymptomatic, and six women had an insufficient clinical response. There was no difference in JZ thickness and uterus volume between the subgroups after UAE (p = 0.912 and 0.531) (see Table 4).

Table 4

Magnetic Resonance Imaging Follow-Up at Baseline and Three Months After Uterine Artery Embolization Between Asymptomatic Women and Women with Insufficient Clinical Response

Parameters	Asymptomatic Women (n=16)	Women with Insufficient Clinical Response (n=6)	P value
JZ thickness (mm)
Baseline	35 (17, 49)	32 (15, 61)	0.854
3-month follow-up	24 (13, 34)	22 (12, 40)	0.912
Reduction	12	10	0.318
Uterus volume (cm3)
Baseline	334 (76, 611)	171 (122, 727)	0.253
3-month follow-up	161 (58, 276)	133 (76, 346)	0.531
Reduction	156	43	0.224

Discussion

In this study, the embolization began with smaller-sized PVA particles (100 μm) to first block the smaller intra-AD vessels and then relatively larger particles (300 μm) were used for the proximal embolization. Only one patient underwent a hysterectomy at 35 months. The preserved uterus rate was 96% (26/27), and the asymptomatic rate was 74% (20/27).

In previous studies, various embolization materials and protocols were used in UAE. However, it is not yet clear which embolization material or protocol can result in better clinical outcomes. Popovic et al7 summarized the results of 15 studies with a total of 511 cases. Improvements in symptoms that primarily related to bleeding, pain, and bulk-related discomfort were reported by 387 patients (75.7%) at the 26.9 months follow-up. The embolization materials included PVA particles, TGM, KMG, and GFP, and the primary sizes were 355–500 μm, 500–700 μm, and 700–900 μm. However, the researchers did not perform a systematic literature search and performed only a limited qualitative assessment, so the symptom improvement rate should be interpreted critically.

De Bruijn et al9 also used the UFS-QoL to measure SS and HRQOL. Embolization was started with Embozene 500 μm first to block the smaller vessels and continued with Embozene 700 μm, followed by Embozene 900 μm, when judged necessary. Five out of 28 women had undergone a secondary hysterectomy before the seven-year follow-up, meaning a hysterectomy was avoided in 23 (82%) cases, and 17 (61%) of the 28 patients were asymptomatic. In a group of 40 patients studied by Smeets et al11 embospheres of 500–700 and 700–900 μm were used as embolic agents, and 83% of the patients had a preserved uterus and 72% of them were asymptomatic at a mean follow-up of 65 months.

The outcomes of this study are better than those of previous studies with medium-sized embolic particles (300–700 µm), especially with respect to the preserved uteri. It is reasonable to assume that smaller-sized particles may induce larger ischemia with a more distal penetration into the lesions, leading to higher infarction and better clinical outcomes.12 Small-sized PVA particles have been reported as safe for UAE without untargeted embolization by Kim et al.13 In the study, UAE with the “1-2-3 protocol” (small PVA particles measuring 150–250 µm were used at the beginning of embolization, followed by 250–355 µm and 355–500 µm particles) resulted in more than 80% of patients with complete necrosis of AD and a lower recurrence rate of symptoms. As in the Kim et al13 and de Bruijn et al9 studies, there were no deaths or other complications after UAE in this study.

This study found no difference in the reduction of SSS and an improvement in HRQOL between patients with pure AD and those with concomitant fibroids. This finding was concordant with the report of Smeets et al.11 In other words, the relief of symptoms and improvement of QoL after UAE was not related to whether the patients had pure AD or AD combined with uterine fibroids.

The study of de Bruijn et al9 shows no HRQOL difference between patients with pure AD and those with AD and fibroids at the seven-year follow-up, but the SSS has a statistical difference in favor of those with AD combined with fibroids. Subsequently, de Bruijn et al14 published a meta-analysis suggesting that combined AD seems to respond better to UAE compared with pure AD in both the short-term and the long-term. Although several case series and cohorts that evaluated UAE as a treatment for symptomatic AD have been reported over the last fifteen years, many of the individual studies did not report all the information necessary to weigh up the quality of the research properly. Thus, there is still a lack of firm evidence that the outcome of UAE for AD combined with fibroids is better than that of pure AD. Confirmation is imperative to studies in related randomized controlled trials.4,15–18

Whether JZ thickness at baseline can be used as a predictor of response is also controversial. De Bruijn’s meta-analysis indicated that greater JZ thickness was associated with a decreased effectiveness in UAE for treating adenomyosis.14 However, a study by Froeling et al19 indicated that treatment failure was not associated with either JZ thickness or the pattern of uterine AD (focal or diffuse). The results of this study are consistent with those of Froeling et al, which do not support the use of JZ thickness at baseline as a predictor for long-term efficacy.

The biggest shortcoming of this retrospective study is that the sample size is too small. Secondly, T1-weighted contrast-enhanced images were absent on the three-month follow-up MRI in some patients, making it impossible to determine whether the percentage of necrosis of AD was related to symptom recurrence in the long-term follow-up.

Conclusions

The follow-up outcomes of this study confirmed that UAE with small particles is safe and effective in treating AD, especially in terms of preserving the uterus. The findings also indicate that there is no difference in clinical improvement between women treated with UAE for pure AD and those treated for AD in combination with fibroids. In addition, JZ thickness at baseline does not seem to be a predictor for the long-term efficacy of UAE.