| Literature DB >> 34290242 |
Sandy R Pernitzsch1,2, Mona Alzheimer1, Belinda U Bremer1, Marie Robbe-Saule3,4, Hilde De Reuse3, Cynthia M Sharma5.
Abstract
The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase variation by also modulating the expression of a gene (hp0102) that is co-transcribed with tlpB. The hp0102 gene encodes a glycosyltransferase required for LPS O-chain biosynthesis and in vivo colonization of the mouse stomach. The G-repeat length defines a gradual (rather than ON/OFF) control of LPS biosynthesis by RepG, and leads to gradual resistance to a membrane-targeting antibiotic. Thus, RepG-mediated modulation of LPS structure might impact host immune recognition and antibiotic sensitivity, thereby helping H. pylori to adapt and persist in the host.Entities:
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Year: 2021 PMID： 34290242 DOI： 10.1038/s41467-021-24689-2
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919