| Literature DB >> 34286469 |
Jill K Badin1, Caleb Eggenberger1,2, Stacey Dineen Rodenbeck1,3, Zubair A Hashmi4, I-Wen Wang4, Jose P Garcia4, Mouhamad Alloosh1, Michael Sturek5.
Abstract
Intracellular free Ca2+ ([Ca2+]i) dysregulation occurs in coronary smooth muscle (CSM) in atherosclerotic coronary artery disease (CAD) of metabolic syndrome (MetS) swine. Our goal was to determine how CAD severity, arterial structure, and MetS risk factors associate with [Ca2+]i dysregulation in human CAD compared to changes in Ossabaw miniature swine. CSM cells were dispersed from coronary arteries of explanted hearts from transplant recipients and from lean and MetS swine with CAD. CSM [Ca2+]i elicited by Ca2+ influx and sarcoplasmic reticulum (SR) Ca2+ release and sequestration was measured with fura-2. Increased [Ca2+]i signaling was associated with advanced age and a greater media area in human CAD. Decreased [Ca2+]i signaling was associated with a greater number of risk factors and a higher plaque burden in human and swine CAD. Similar [Ca2+]i dysregulation exhibited in human and Ossabaw swine CSM provides strong evidence for the translational relevance of this large animal model.Entities:
Keywords: Animal model; Atherosclerosis; Ca2+ influx; Ca2+ release; Histology; Metabolic syndrome; Obesity; Risk factors; Sarco-endoplasmic reticulum Ca2+ ATPase
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Year: 2021 PMID： 34286469 DOI： 10.1007/s12265-021-10153-5
Source DB: PubMed Journal: J Cardiovasc Transl Res ISSN： 1937-5387 Impact factor: 4.132