Literature DB >> 34286037

Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining.

Hiroyuki Ohsaki1, Toru Matsunaga2, Taishi Fujita3, Yasunori Tokuhara4, Shingo Kamoshida1, Tadashi Sofue5.   

Abstract

In glomerular disease, podocytes and parietal epithelial cells (PECs) are shed in the urine. Therefore, urinary podocytes and PECs are noninvasive biomarkers of glomerular disease. The purpose of this protocol is to employ immunocytochemistry to detect podocytes and PECs, using the WT1 antibody on liquid-based cytology slides.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Crescent formation; Glomerular disease; Immunocytochemistry; Liquid-based cytology; Parietal epithelial cells; Podocytes; Urine; WT1

Year:  2018        PMID： 34286037      PMCID： PMC8275290          DOI： 10.21769/BioProtoc.2827

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  De novo expression of podocyte proteins in parietal epithelial cells in experimental aging nephropathy.

Authors:  Jiong Zhang; Kim M Hansen; Jeffrey W Pippin; Alice M Chang; Yoshinori Taniguchi; Ronald D Krofft; Scott G Pickering; Zhi-Hong Liu; Christine K Abrass; Stuart J Shankland
Journal:  Am J Physiol Renal Physiol       Date:  2011-11-30

Review 2.  Immunocytochemistry: an indispensable technique in routine cytology.

Authors:  L Skoog; E Tani
Journal:  Cytopathology       Date:  2011-08       Impact factor: 2.073

3.  Urinary WT1-positive cells as a non-invasive biomarker of crescent formation.

Authors:  T Fujita; T Sofue; M Moritoki; Y Nishijima; Y Tokuhara; H Wakisaka; Y Kushida; R Haba; H Ohsaki
Journal:  Cytopathology       Date:  2017-09-15       Impact factor: 2.073

4.  Urinary excretion of podocytes reflects disease activity in children with glomerulonephritis.

Authors:  M Hara; T Yanagihara; T Takada; M Itoh; M Matsuno; T Yamamoto; I Kihara
Journal:  Am J Nephrol       Date:  1998       Impact factor: 3.754

5.  WT1 immunoenzyme staining using SurePath(™) processed urine cytology helps to detect kidney disease.

Authors:  H Ohsaki; T Sofue; K Kawakami; Y Nishijima; T Hara; T Matsunaga; Y Kushida; R Haba; Y Shigematsu; S Irino; Y Norimatsu
Journal:  Cytopathology       Date:  2015-02-14       Impact factor: 2.073

6.  Urinary excretion of podocytes in patients with diabetic nephropathy.

Authors:  T Nakamura; C Ushiyama; S Suzuki; M Hara; N Shimada; I Ebihara; H Koide
Journal:  Nephrol Dial Transplant       Date:  2000-09       Impact factor: 5.992

7.  Parietal epithelia cells in the urine as a marker of disease activity in glomerular diseases.

Authors:  Johannes Achenbach; Michael Mengel; Irini Tossidou; Imke Peters; Joon-Keun Park; Marion Haubitz; Jochen H Ehrich; Hermann Haller; Mario Schiffer
Journal:  Nephrol Dial Transplant       Date:  2008-05-05       Impact factor: 5.992

8.  The Wilms' tumour protein (WT1) shuttles between nucleus and cytoplasm and is present in functional polysomes.

Authors:  Martina Niksic; Joan Slight; Jeremy R Sanford; Javier F Caceres; Nicholas D Hastie
Journal:  Hum Mol Genet       Date:  2003-12-17       Impact factor: 6.150
  8 in total

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