Literature DB >> 34286033

Isolation and Maintenance of Murine Embryonic Striatal Neurons.

Luana Naia1, A Cristina Rego1,2.   

Abstract

Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell connectivity and the presence of dendritic spines, which reflects the maturation of the network.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Medium spiny neurons; Primary cultures; Striatal neurons; Striatum dissection

Year:  2018        PMID： 34286033      PMCID： PMC8275303          DOI： 10.21769/BioProtoc.2823

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  The frequency and distribution of medium-sized neurons with indented nuclei in the primate and rodent neostriatum.

Authors:  G A Graveland; M DiFiglia
Journal:  Brain Res       Date:  1985-02-18       Impact factor: 3.252

2.  Cortico-striatal synaptic defects and OCD-like behaviours in Sapap3-mutant mice.

Authors:  Jeffrey M Welch; Jing Lu; Ramona M Rodriguiz; Nicholas C Trotta; Joao Peca; Jin-Dong Ding; Catia Feliciano; Meng Chen; J Paige Adams; Jianhong Luo; Serena M Dudek; Richard J Weinberg; Nicole Calakos; William C Wetsel; Guoping Feng
Journal:  Nature       Date:  2007-08-23       Impact factor: 49.962

3.  Differential loss of striatal projection neurons in Huntington disease.

Authors:  A Reiner; R L Albin; K D Anderson; C J D'Amato; J B Penney; A B Young
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

4.  Non-Neuronal Cells Are Required to Mediate the Effects of Neuroinflammation: Results from a Neuron-Enriched Culture System.

Authors:  Chin Wai Hui; Yang Zhang; Karl Herrup
Journal:  PLoS One       Date:  2016-01-20       Impact factor: 3.240

5.  Striatal medium-sized spiny neurons: identification by nuclear staining and study of neuronal subpopulations in BAC transgenic mice.

Authors:  Miriam Matamales; Jesus Bertran-Gonzalez; Lucas Salomon; Bertrand Degos; Jean-Michel Deniau; Emmanuel Valjent; Denis Hervé; Jean-Antoine Girault
Journal:  PLoS One       Date:  2009-03-10       Impact factor: 3.240
  5 in total
  1 in total

1.  Restored Fyn Levels in Huntington's Disease Contributes to Enhanced Synaptic GluN2B-Composed NMDA Receptors and CREB Activity.

Authors:  Lígia Fão; Patrícia Coelho; Ricardo J Rodrigues; A Cristina Rego
Journal:  Cells       Date:  2022-09-29       Impact factor: 7.666
  1 in total

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