Literature DB >> 34286027

Determination of Intracellular Osmolytes in Cyanobacterial Cells.

Xiaoming Tan1, Kuo Song1, Cuncun Qiao1, Xuefeng Lu1,2.   

Abstract

Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of cyanobacteria, filamentous cyanobacterium Anabaena sp. PCC 7120, unicellular cyanobacterium Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942 and halotolerant unicellular cyanobacterium Synechococcus sp. PCC 7002.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Cyanobacteria; Glucosylglycerol; Ion chromatography; Osmolyte; Sucrose; Synechocystis

Year:  2018        PMID： 34286027      PMCID： PMC8275283          DOI： 10.21769/BioProtoc.2812

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

Review 1.  Compatible solute biosynthesis in cyanobacteria.

Authors:  Stephan Klähn; Martin Hagemann
Journal:  Environ Microbiol       Date:  2010-11-05       Impact factor: 5.491

2.  Capillary ion chromatography-mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria.

Authors:  Yun Fa; Wenhui Liang; He Cui; Yangkai Duan; Menglong Yang; Jun Gao; Huizhou Liu
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2015-07-31       Impact factor: 3.205

3.  Exploring the photosynthetic production capacity of sucrose by cyanobacteria.

Authors:  Wei Du; Feiyan Liang; Yangkai Duan; Xiaoming Tan; Xuefeng Lu
Journal:  Metab Eng       Date:  2013-05-28       Impact factor: 9.783

Review 4.  Diversity, distribution and roles of osmoprotective compounds accumulated in halophytes under abiotic stress.

Authors:  Inès Slama; Chedly Abdelly; Alain Bouchereau; Tim Flowers; Arnould Savouré
Journal:  Ann Bot       Date:  2015-01-05       Impact factor: 4.357

5.  Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria.

Authors:  Xiaoming Tan; Lun Yao; Qianqian Gao; Weihua Wang; Fengxia Qi; Xuefeng Lu
Journal:  Metab Eng       Date:  2011-01-08       Impact factor: 9.783

6.  The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.

Authors:  M Hagemann; S Richter; S Mikkat
Journal:  J Bacteriol       Date:  1997-02       Impact factor: 3.490

7.  Rapid and sensitive NMR method for osmolyte determination.

Authors:  Andrea Motta; Ida Romano; Agata Gambacorta
Journal:  J Microbiol Methods       Date:  2004-08       Impact factor: 2.363

8.  Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942.

Authors:  Cuncun Qiao; Yangkai Duan; Mingyi Zhang; Martin Hagemann; Quan Luo; Xuefeng Lu
Journal:  Appl Environ Microbiol       Date:  2018-01-02       Impact factor: 4.792

9.  The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production.

Authors:  Kuo Song; Xiaoming Tan; Yajing Liang; Xuefeng Lu
Journal:  Appl Microbiol Biotechnol       Date:  2016-04-14       Impact factor: 4.813

10.  The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp. PCC 6803.

Authors:  Kuo Song; Martin Hagemann; Xiaoming Tan; Xuefeng Lu
Journal:  Front Microbiol       Date:  2017-06-26       Impact factor: 5.640
  10 in total

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