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Literature DB >> 34286023

Quantification of Thrips Damage Using Ilastik and ImageJ Fiji.

Isabella G S Visschers¹, Nicole M Van Dam^1,2,3, Janny L Peters⁴.

Abstract

Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips).

Entities: Chemical

Keywords: Image analyses; Insect damage; Insect resistance

Year: 2018 PMID： 34286023 PMCID： PMC8275258 DOI： 10.21769/BioProtoc.2806

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

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Quantification of Thrips Damage Using Ilastik and ImageJ Fiji.

1. Birds and bats reduce insect biomass and leaf damage in tropical forest restoration sites.

2. Fiji: an open-source platform for biological-image analysis.

3. Mechanical stiffness as an improved single-cell indicator of osteoblastic human mesenchymal stem cell differentiation.

4. Metabolomic analysis of host plant resistance to thrips in wild and cultivated tomatoes.

5. NMR metabolomics of thrips (Frankliniella occidentalis) resistance in Senecio hybrids.

6. Tubular endocytosis drives remodelling of the apical surface during epithelial morphogenesis in Drosophila.