Literature DB >> 34286016

Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple.

Aparecida L Silva1, Keini Dressano1, Paulo H O Ceciliato1, Juan Carlos Guerrero-Abad1, Daniel S Moura1.   

Abstract

The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, Arabidopsis seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol purple, treated with the peptide and photographed after 30 min. Herein the protocol is optimized for evaluation of exogenous treatment, described in detail and expected results are presented.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Alkalinization; Bromocresol purple; RALF; pH indicator

Year:  2018        PMID： 34286016      PMCID： PMC8275321          DOI： 10.21769/BioProtoc.2796

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  Production of multiple plant hormones from a single polyprotein precursor.

Authors:  G Pearce; D S Moura; J Stratmann; C A Ryan
Journal:  Nature       Date:  2001-06-14       Impact factor: 49.962

2.  Biological activity of nine recombinant AtRALF peptides: implications for their perception and function in Arabidopsis.

Authors:  Amanda Morato do Canto; Paulo H O Ceciliato; Bianca Ribeiro; Fausto Andrés Ortiz Morea; Antonio Augusto Franco Garcia; Marcio C Silva-Filho; Daniel S Moura
Journal:  Plant Physiol Biochem       Date:  2013-12-14       Impact factor: 4.270

3.  A peptide hormone and its receptor protein kinase regulate plant cell expansion.

Authors:  Miyoshi Haruta; Grzegorz Sabat; Kelly Stecker; Benjamin B Minkoff; Michael R Sussman
Journal:  Science       Date:  2014-01-24       Impact factor: 47.728

4.  RALF, a 5-kDa ubiquitous polypeptide in plants, arrests root growth and development.

Authors:  G Pearce; D S Moura; J Stratmann; C A Ryan
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

5.  A fungal pathogen secretes plant alkalinizing peptides to increase infection.

Authors:  Sara Masachis; David Segorbe; David Turrà; Mercedes Leon-Ruiz; Ursula Fürst; Mennat El Ghalid; Guy Leonard; Manuel S López-Berges; Thomas A Richards; Georg Felix; Antonio Di Pietro
Journal:  Nat Microbiol       Date:  2016-04-11       Impact factor: 17.745

6.  NaRALF, a peptide signal essential for the regulation of root hair tip apoplastic pH in Nicotiana attenuata, is required for root hair development and plant growth in native soils.

Authors:  Jinsong Wu; Erin L Kurten; Gabriele Monshausen; Grégoire M Hummel; Simon Gilroy; Ian T Baldwin
Journal:  Plant J       Date:  2007-10-03       Impact factor: 6.417

7.  Regulation and processing of a plant peptide hormone, AtRALF23, in Arabidopsis.

Authors:  Renu Srivastava; Jian-Xiang Liu; Hongqing Guo; Yanhai Yin; Stephen H Howell
Journal:  Plant J       Date:  2009-05-18       Impact factor: 6.417

8.  BAK1 is involved in AtRALF1-induced inhibition of root cell expansion.

Authors:  Keini Dressano; Paulo H O Ceciliato; Aparecida L Silva; Juan Carlos Guerrero-Abad; Tábata Bergonci; Fausto Andrés Ortiz-Morea; Marco Bürger; Marcio C Silva-Filho; Daniel S Moura
Journal:  PLoS Genet       Date:  2017-10-13       Impact factor: 5.917
  8 in total
  1 in total

1.  Aquaporin family lactic acid channel NIP2;1 promotes plant survival under low oxygen stress in Arabidopsis.

Authors:  Zachary G Beamer; Pratyush Routray; Won-Gyu Choi; Margaret K Spangler; Ansul Lokdarshi; Daniel M Roberts
Journal:  Plant Physiol       Date:  2021-12-04       Impact factor: 8.005
  1 in total

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