Literature DB >> 34285965

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana.

Valérie Allasia1, Benoit Industri1, Michel Ponchet1, Michaël Quentin1, Bruno Favery1, Harald Keller1.   

Abstract

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis thaliana; Defense hormone; HPLC; SA-glucoside; Salicylic acid

Year:  2018        PMID： 34285965      PMCID： PMC8275313          DOI： 10.21769/BioProtoc.2844

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  Localization, conjugation, and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus.

Authors:  A J Enyedi; N Yalpani; P Silverman; I Raskin
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

2.  Ortho-anisic acid as internal standard for the simultaneous quantitation of salicylic acid and its putative biosynthetic precursors in cucumber leaves.

Authors:  P Meuwly; J P Métraux
Journal:  Anal Biochem       Date:  1993-11-01       Impact factor: 3.365

3.  Novel plant immune-priming compounds identified via high-throughput chemical screening target salicylic acid glucosyltransferases in Arabidopsis.

Authors:  Yoshiteru Noutoshi; Masateru Okazaki; Tatsuya Kida; Yuta Nishina; Yoshihiko Morishita; Takumi Ogawa; Hideyuki Suzuki; Daisuke Shibata; Yusuke Jikumaru; Atsushi Hanada; Yuji Kamiya; Ken Shirasu
Journal:  Plant Cell       Date:  2012-09-07       Impact factor: 11.277

4.  The Arabidopsis microtubule-associated protein MAP65-3 supports infection by filamentous biotrophic pathogens by down-regulating salicylic acid-dependent defenses.

Authors:  Michaël Quentin; Isabelle Baurès; Caroline Hoefle; Marie-Cécile Caillaud; Valérie Allasia; Franck Panabières; Pierre Abad; Ralph Hückelhoven; Harald Keller; Bruno Favery
Journal:  J Exp Bot       Date:  2016-01-21       Impact factor: 6.992

5.  Differences in salicylic acid glucose conjugations by UGT74F1 and UGT74F2 from Arabidopsis thaliana.

Authors:  Alayna M George Thompson; Cristina V Iancu; Kenneth E Neet; John V Dean; Jun-Yong Choe
Journal:  Sci Rep       Date:  2017-04-20       Impact factor: 4.379
  5 in total

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