Electrochemical fabrication of TiO2 micro-flowers for an efficient intracellular delivery using nanosecond light pulse.

Introduction of foreign cargo into the targeted living cell with high transfection efficiency and high cell viability is an important mean for many biological and biomedical research purpose. Here, we have demonstrated a newly developed Titanium oxide micro-flower structure (TMS) for intracellular delivery. The TMS were formed on titanium (Ti) substrate using an electrochemical anodization process. The TMS consists of branches of titanium dioxide (TiO2) nanotubes, which play an important role in efficient cargo delivery. Due to nanosecond pulse laser exposure, Ti substrate heat-up, generating cavitation bubbles. These bubbles can rapidly grow, coalesce, and collapse to induce explosion resulting in very strong fluid flow through the TiO2 nanotubes and disrupt the cell plasma membrane promoting the delivery of biomolecules into cells. Using this platform, we successfully deliver dyes with 93% efficiency and nearly 98% cell viability into HCT cells, and this technique is potentially applicable for cellular therapy and diagnostics.