| Literature DB >> 34284713 |
Nadezhda V Zyrina1,2, Valeriya N Antipova3.
Abstract
The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.Keywords: DNA polymerases; EXPAR; LAMP; SDA; background synthesis; isothermal amplification
Year: 2021 PMID： 34284713 DOI： 10.1134/S0006297921070099
Source DB: PubMed Journal: Biochemistry (Mosc) ISSN： 0006-2979 Impact factor: 2.487