Viliam Šnábel1, Tetiana A Kuzmina2, Anatoliy A Antipov3, Oleksandr M Yemets4, Serena Cavallero5, Martina Miterpáková6, Stefano D'Amelio5, Daniela Antolová6, Zuzana Vasilková6, Rusłan Sałamatin7,8. 1. Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 040 01, Košice, Slovakia. snabel@saske.sk. 2. I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Kyiv, 01030, Ukraine. 3. Bila Tserkva National Agrarian University, Soborna sq., 8/1, Bila Tserkva, 09117, Kyiv Region, Ukraine. 4. Sumy National Agrarian University, G. Kondratieva 160, Sumy, Ukraine. 5. Department of Public Health and Infectious Diseases, Section of Parasitology, Sapienza University of Rome, 00185, Rome, Italy. 6. Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 040 01, Košice, Slovakia. 7. Department of General Biology and Parasitology, Medical University of Warsaw, Chałubińskiego 5, 02-004, Warsaw, Poland. 8. Faculty of Medicine, Collegium Medicum, Cardinal Stefan Wyszyński University in Warsaw, st. Kazimierza Wóycickiego 1/3, 01-938, Warsaw, Poland.
Abstract
INTRODUCTION: Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission. METHODS: Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789 bp), NADH dehydrogenase 1 (602 bp) and 12S rRNA (333-334 bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine). RESULTS: Four isolates from four pigs in the Sumy region were identified as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classified as E. granulosus s.s. (G1 genotype), including one microvariant (G1A). CONCLUSION: To our knowledge, this is the first genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The finding has implications for public health as local control programmes should take into consideration different development rate of this parasite in dogs and the greater risk of the species for human infection.
INTRODUCTION: Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission. METHODS: Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789 bp), NADH dehydrogenase 1 (602 bp) and 12S rRNA (333-334 bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine). RESULTS: Four isolates from four pigs in the Sumy region were identified as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classified as E. granulosus s.s. (G1 genotype), including one microvariant (G1A). CONCLUSION: To our knowledge, this is the first genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The finding has implications for public health as local control programmes should take into consideration different development rate of this parasite in dogs and the greater risk of the species for human infection.
Authors: Benjamin K Mogoye; Colin N Menezes; Michelle L Wong; Sarah Stacey; Dirk von Delft; Kerstin Wahlers; Marion Wassermann; Thomas Romig; Peter Kern; Martin P Grobusch; John Frean Journal: Vet Parasitol Date: 2013-04-10 Impact factor: 2.738