hsa_circ_0101119 facilitates the progression of cervical cancer via an interaction with EIF4A3 to inhibit TCEAL6 expression.

Recently, circular RNAs (circRNAs/circs) have attracted increased attention due to their regulatory role in a variety of cancer types. However, the role and molecular mechanisms of circRNAs in cervical cancer (CC) remain unknown. The present study aimed to investigate the function of hsa_ circ_0101119 on CC and its potential mechanisms. The differentially expressed circRNAs associated with CC were screened out using R software, according to the database of Gene Expression Omnibus (GEO). The expression levels of hsa_circ_0101119, eukaryotic initiation factor 4A‑3 (EIF4A3) and transcription elongation factor A‑like 6 (TCEAL6) in CC cells were detected via reverse transcription‑quantitative (RT‑q)PCR, and their expression levels in CC tissues were analyzed based on the database of GEO and the Cancer Genome Atlas. Moreover, the accurate functions of hsa_circ_0101119 and TCEAL6 on the proliferation, apoptosis, migration and invasion of SiHa and HeLa cells was examined using colony formation assay, 5‑ethynyl‑20‑deoxyuridine incorporation assay, flow cytometry and Transwell assay. Next, the underlying mechanisms of hsa_circ_0101119 on CC progression were determined via bioinformatics analysis, RNA immunoprecipitation assay, RNA pull down assay, RT‑qPCR and western blotting. It was found that hsa_circ_0101119 was highly expressed in CC tissues and cells, while TCEAL6 was lowly expressed. Knockdown of hsa_circ_0101119 or TCEAL6 overexpression significantly inhibited the proliferation, migration and invasion of SiHa and HeLa cells, but facilitated apoptosis. It was also demonstrated that hsa_circ_0101119 could recruit EIF4A3 to inhibit TCEAL6 expression in CC. Furthermore, knockdown of TCEAL6 could reverse the effects of silencing hsa_circ_0101119 on the proliferation, apoptosis, migration and invasion of HeLa cells. In conclusion, the present study revealed that hsa_circ_0101119 could facilitate cell proliferation, migration and invasion, and suppress apoptosis in CC via an interaction with EIF4A3 to inhibit TCEAL6 expression, which may provide a potential therapeutic target for CC treatment.

Introduction

Cervical cancer (CC) is one of the most common gynecological neoplasms among women worldwide (1). The incidence and mortality rates of CC have been increasing every year in developed and developing countries (2,3). Despite the fact that encouraging improvements have been achieved in CC chemotherapy, radiotherapy and surgical therapies, the 5-year survival rate of patients with CC remains unsatisfactory (4). Therefore, it is essential to identify effective therapeutic targets and discover molecular mechanisms in CC progression.

Circular RNAs (circRNAs/circs), a novel class of non-coding RNAs, are featured with a continuous closed loop without 5′-3′ polarity or a polyadenylated tail (5). In recent years, accumulating evidence has confirmed that circRNAs have significant implications in numerous tumors (6). circRNAs have been reported to be involved in the biological behaviors of tumor cells, including proliferation, survival, apoptosis and metastasis (7,8). For example, hsa_circ_0000263 facilitates CC progression by regulating CC cell proliferation and migration (9). Ou et al (10) revealed that circ_angiomotin like 1 (AMOTL1) could promote CC growth by promoting AMOTL1 expression. Moreover, a study by Wang et al (11) reported that hsa_circ_0101119 expression was notably upregulated in the peripheral blood of patients with cervical squamous cell carcinoma. However, to the best of our knowledge, the role of hsa_circ_0101119 on CC has not yet been evaluated.

Recently, an increasing number of researchers have paid attention to the application of circRNAs in cancer diagnosis (12,13). The main mechanisms via which circRNAs serve a role in cancer are by directly interacting with microRNAs (miRNAs) or RNA binding proteins (RBPs) (8,14–16). Eukaryotic initiation factor 4A-3 (EIF4A3) is one of the RBPs that is a core component of the exon junction complex (17). The roles of EIF4A3 are reported to be complex and important in numerous diseases, and the abnormal structure and function of EIF4A3 directly lead to the changes of its downstream biological effects (18).

Members of the transcription elongation factor A-like (TCEAL) gene family contain TFA domains and may function as nuclear phosphoproteins that modulate transcription in a promoter context-dependent manner (19–21). At present, TCEAL7 is frequently deregulated in tumors, and its decreased expression often correlates with malignant clinical process and poor prognosis in multiple cancer types, including gastric cancer and non-small cell lung cancer (22,23). Moreover, Chien et al (24) revealed that TCEAL6 has a sequence similarity to TCEAL7. Interestingly, TCEAL6 is reported to be lowly expressed in the early stage of CC (25). However, whether hsa_circ_0101119 affects the progression of CC via an interaction with EIF4A3 to regulate TCEAL6 expression remains unknown. The present study aimed to investigate the function of hsa_circ_0101119 in CC, and its regulatory mechanism that is associated with EIF4A3 and TCEAL6.

Materials and methods

Bioinformatics analysis

Gene expression data matrix (GSE102686) was derived from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/), which included five CC samples and five adjacent non-tumorous samples (26). The present study identified the top five hsa_circRNAs with the highest differential expression, and selected hsa_circ_0101119 as the research target. Then, RNA-Protein Interaction Prediction (RPISeq; version 1.0; http://pridb.gdcb.iastate.edu/RPISeq/) was used to predict the interaction probabilities of RNA-binding protein EIF4A3 with hsa_circ_0101119 and TCEAL6.

Cell culture and transfection

The four human CC cell lines (C-33A, SiHa, CaSki and HeLa) and the normal human cervical epithelial cell line, HcerEpic, were supplied by VCANBIO Cell & Gene Engineering Co., Ltd. All the cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) FBS (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.), and cultured at 37°C in a humidified 5% CO2 incubator.

Short hairpin (sh)RNA targeting EIF4A3 (sh-EIF4A3) and TCEAL6 (sh-TCEAL6), small interfering (si)RNA targeting hsa_circ_0101119 (si-hsa_circ), pcDNA3.1-TCEAL6 (pcDNA-TCEAL6) and their correspond negative controls (sh-NC, si-NC and pcDNA-NC) were purchased from Shanghai GenePharma Co., Ltd. The sh-NC was a plasmid containing a non-targeting (scramble) shRNA sequences, and the si-NC was a non-targeting (scramble) siRNA sequence. The sequences were as follows: Sh-EIF4A3, 5′-GGAAGACATGACTAAAGTGGA−3′; sh-TCEAL6, 5′-GGAGAAGGGATCCGGTAGATT−3′; sh-NC, 5′-GGTAGTGGACGATGAGACAGT−3′; si-hsa_circ, 5′-ATGAGCAGCCATACACTGCTT−3′; si-NC, 5′-GCTCTACTTCGACGACAAGAT−3′. According to the manufacturer's instruction, the cells were transfected with sh-EIF4A3 (20 µl/ml), sh-TCEAL6 (20 µl/ml), si-hsa_circ (50 nM) or pcDNA-TCEAL6 (4 µg) using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 48 h. Finally, the transfection efficiency was determined via reverse transcription-quantitative PCR (RT-qPCR).

Colony formation assay

The transfected SiHa and HeLa cells (500 cells/well) were seeded into 6-well plates. Then, the cells were grown for 14 days at 37°C. Next, 4% paraformaldehyde was used to fix colonies for 15 min at room temperature and 0.1% crystal violet was used to stain colonies for 30 min at room temperature. Finally, the numbers of colonies containing >500 cells were assessed using a light microscope (magnification, ×100).

5-Ethynyl-20-deoxyuridine (EdU) incorporation assay

The EdU assay was carried out with the EdU labeling/detection kit (Guangzhou RiboBio Co., Ltd.) according to the manufacturer's instructions. The transfected SiHa and HeLa cells were incubated with 50 µM EdU solution for 2 h at room temperature. Then, the cells were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by permeabilized with 0.5% Triton X-100 at room temperature and stained with anti-EdU working solution for 30 min in the dark at room temperature. Finally, DAPI (Sigma-Aldrich; Merck KGaA) was used for staining the cell nucleus at room temperature for 30 min, and the EdU-positive cells were observed using fluorescent microscopy (magnification, ×200).

Flow cytometry

The apoptosis of SiHa and HeLa cells was detected using the Annexin V-PI kit (Beyotime Institute of Biotechnology). The transfected SiHa and HeLa cells were collected and resuspended in the binding buffer. Subsequently, the cells were labeled with Annexin V-FITC and PI. Finally, cell apoptosis was detected using a flow cytometry (FACSCalibur; BD Biosciences), and then data were analyzed using FlowJo software (version v7.6.5; FlowJo LLC).

Transwell assay

Transwell chambers with 8-µm pores were obtained from Corning, Inc. A total of 48 h after transfection, the SiHa and HeLa cells were suspended into serum-free medium and seeded into the upper chamber without Matrigel (BD Biosciences). Next, 500 µl medium with 10% FBS was added to the lower chamber as the chemoattractant. After a 24-h incubation at room temperature, the migrated cells were fixed with methanol at 37°C for 30 min and stained with 0.1% crystal violet at 37°C for 30 min, following which they were imaged and calculated using an inverted microscope (magnification, ×100). Moreover, the cell invasion assay was performed at the same time with the aforementioned steps, except that the upper chamber was coated with Matrigel at room temperature overnight.

RNA immunoprecipitation (RIP) assay

The RIP assay was performed using an EZ-Magna RIP™ RNA kit (MilliporeSigma) to determine the endogenous interaction between EIF4A3 and hsa_circ_0101119 or TCEAL6, according to the manufacturer's protocols. Briefly, the cells were cross-linked by treating with formaldehyde for 10 min at room temperature. After washing with cold PBS, cells were incubated in 4 ml cell lysis buffer for 15 min in ice. After nuclear extraction using a Dounce homogenizer (Wheaton; DWK Life Sciences), the chromatin was sheared by sonication (25% power, 4.5 sec impact, 9 sec clearance, 14 times) at room temperature to obtain DNA fragments in a range between 1,000-200 bp. Following DNase treatment for 30 min at 37°C, the cell lysates were incubated with RIP buffer containing the magnetic beads conjugated with anti-EIF4A3 antibody for 2 h at 4°C with gently shaking. The anti-IgG antibody served as the control. The immunoprecipitated RNA was released by the reverse cross-linking using proteinase K at 4°C overnight. Finally, the immunoprecipitated RNA was extracted using a RNeasy Min Elute Cleanup kit (Qiagen, Inc.) and then analyzed using an RT-qPCR assay.

RNA pull down assay

The interaction between hsa_circ_0101119 and EIF4A3 was assessed using the Magnetic RNA-Protein Pull-Down kit (Pierce; Thermo Fisher Scientific, Inc.), following the manufacturer's instructions. Biotin-labeled hsa_circ_0101119 or antisense RNA, which was supplied by Genepharm, Inc., was co-incubated with the magnetic beads and the protein extract of SiHa or Hela cells. The pull-down protein was measured via western blotting.

RT-qPCR

Total RNA from cells was extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions. Subsequently, cDNA was synthesized and amplified from total RNA (2 µg) using the One Step PrimeScript cDNA Synthesis kit (Takara Bio, Inc.) according to the manufacturer's protocols. Then, cDNA samples (1 µl) were subjected to a qPCR reaction system in presence of SYBR Premix Ex Taq II kit (Takara Bio, Inc.). GAPDH was used as an endogenous reference. All primers were designed by Shanghai GenePharma Co., Ltd. and were as follows: Hsa_circ_0101119 forward, 5′-AAGCACACCAGCTTCTCCTC-3′ and reverse, 5′-GCGTGCTGGCATAGGATTTG-3′; EIF4A3 forward, 5′-CCCTCACCACAATGACAGCA-3′ and reverse, 5′-TGACCCACGCAGGTTAAACA-3′; TCEAL6 forward, 5′-CAGCCGCCATTTCTTTCCAG-3′ and reverse, 5′-GGAAACATCTGACCTCCGCA-3′; and GAPDH forward, 5′-GAAGGTGAAGGTCGGAGTC-3′ and reverse, 5′-GAAGATGGTGATGGGATTTC-3′. The relative expression levels were calculated using the 2−ΔΔCq method (27). The RT-qPCR reaction conditions were as follows: Initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 10 sec, 60°C for 30 sec and 72°C for 30 sec.

Western blot analysis

Total proteins from transfected SiHa and HeLa cells were extracted using the cell lysis buffer (Cell Signaling Technology, Inc.) with a protease inhibitor. An enhanced BCA protein assay kit (Beyotime Institute of Biotechnology) was used to quantify the protein concentration. The protein sample (50 µg) was separated by 10% SDS-PAGE, electrophoretically transferred onto PVDF membranes (MilliporeSigma) and blocked with 5% skimmed milk at room temperature for 2 h. Then, the membrane was incubated with the primary antibody overnight at 4°C and then incubated with the HRP-labeled secondary antibody (anti-mouse IgG, cat. no. 7076, 1:5,000; anti-rabbit IgG, cat. no. 7074, 1:5,000) at room temperature for 2 h. Finally, the bands were visualized using an ECL detection kit (Thermo Fisher Scientific, Inc.). The following antibodies were used: Bax (cat. no. 2772; 1:1,000), caspase-3 (cat. no. 14220; 1:1,000), Bcl-2 (cat. no. 3498; 1:1,000), E-cadherin (cat. no. 3195; 1:500), N-cadherin (cat. no. 14215; 1:1,000), Vimentin (cat. no. 5741; 1:1,000) from Cell Signaling Technology, Inc.; MMP-2 (cat. no. ab92536; 1:1,000), MMP-9 (cat. no. ab228402; 1:500), EIF4A3 (cat. no. ab180573; 1:500) from Abcam; and TCEAL6 (cat. no. ag11732; 1:500) from ProteinTech Group, Inc. The density of the protein bands was semi-quantitated using ImageJ software (version V1.8.0; National Institutes of Health).

Statistical analysis

Data are presented as the mean ± SEM of ≥3 experiments. All data in this study were examined using SPSS 22.0 software (IBM, Corp.) and GraphPad Prism 8.0 software (GraphPad Software, Inc.). The differences between groups were assessed using an unpaired or paired Student's t-test or one-way ANOVA with a Tukey's post hoc test. The correlation between EIF4A3 and TCEAL6 was analyzed via Pearson correlation analysis. P<0.05 was considered to indicate a statistically significant difference.

Results

hsa_circ_0101119 is highly expressed in CC tissues and cells

To investigate circRNA effects in CC, the most differentially expressed circRNAs were analyzed in GSE102686 (Fig. 1A). hsa_circ_0101119 was found to be the most significantly upregulated circRNA (Fig. 1A). Moreover, it was identified that the expression level of hsa_circ_0101119 was notably upregulated in CC cells and tissues (Fig. 1B and C), further suggesting that hsa_circ_0101119 was highly expressed in CC.

Figure 1.

hsa_circ_0101119 is highly expressed in CC tissues and cells. (A) Heatmap of differentially expressed circRNAs in CC tissues and normal tissues according to the online data set (GSE102686). (B) Expression level of hsa_circ_0101119 was detected via reverse transcription-quantitative PCR in CC cell lines (C-33A, SiHa, CaSki and HeLa) and normal human cervical epithelial cell line, HcerEpic. **P<0.01 vs. HcerEpic cells. (C) Expression level of hsa_circ_0101119 in CC tissues and normal tissues, according to the online data set (GSE102686). circRNA/circ, circular RNA.

Knockdown of hsa_circ_0101119 inhibits the proliferation of SiHa and HeLa cells

As shown in Fig. 2A, RT-qPCR was used to verify the transfection efficiency of hsa_circ_0101119 knockdown. Then, the effects of hsa_circ_0101119 on CC cells were assessed using EdU and colony formation assays. The results of EdU assay demonstrated that hsa_circ_0101119 knockdown markedly reduced the proliferation of SiHa and HeLa cells (Fig. 2B). Moreover, colony formation assay results suggested that the cell clones of SiHa and HeLa cells were significantly decreased after knockdown of hsa_circ_0101119 (Fig. 2C). Collectively, the data indicated that the knockdown of hsa_circ_0101119 could inhibit the proliferation of SiHa and HeLa cells.

Figure 2.

Knockdown of hsa_circ_0101119 inhibits the proliferation of SiHa and HeLa cells. (A) Expression level of hsa_circ_0101119 was detected via reverse transcription-quantitative PCR in transfected SiHa and HeLa cells. (B) Proliferation of SiHa and HeLa cells was determined using an EdU assay after transfection with si-hsa_circ_0101119 (magnification, ×200). (C) Colonies numbers of SiHa and HeLa cells were detected using a colony formation assay after transfection with si-hsa_circ_0101119 (magnification, ×100). **P<0.01 vs. si-NC group. NC, negative control; si, small interfering RNA; circ, circular RNA; EdU, 5-ethynyl-20-deoxyuridine.

Knockdown of hsa_circ_0101119 facilitates the apoptosis of SiHa and HeLa cells

The results of flow cytometry demonstrated that the knockdown of hsa_circ_0101119 significantly increased the apoptosis of SiHa and HeLa cells (Fig. 3A). In addition, the expression levels of apoptosis-associated proteins (Bax, caspase-3 and Bcl-2) were detected via western blot analysis. As shown in Fig. 3B, the expression levels of Bax and caspase-3 in SiHa and HeLa cells were markedly upregulated after knockdown of hsa_circ_0101119, but Bcl-2 expression was significantly downregulated. These findings suggested that the knockdown of hsa_circ_0101119 could facilitate the apoptosis of SiHa and HeLa cells.

Figure 3.

Knockdown of hsa_circ_0101119 facilitates the apoptosis of SiHa and HeLa cells. (A) After transfection with si-hsa_circ_0101119, flow cytometry was used to detect the apoptosis of SiHa and HeLa cells. (B) After transfection with si-hsa_circ_0101119, western blotting was performed to measure the expression levels of Bax, caspase-3 and Bcl-2 in SiHa and HeLa cells. **P<0.01 vs. si-NC group. NC, negative control; si, small interfering RNA; circ, circular RNA.

Knockdown of hsa_circ_0101119 inhibits the migration and invasion of SiHa and HeLa cells

Knockdown of hsa_circ_0101119 significantly decreased the migration and invasion of SiHa and HeLa cells (Fig. 4A and B). MMPs, such as MMP-2 and MMP-9, which are secreted by cells, are reported to promote cancer cell invasion and migration (28). Thus, the effects of hsa_circ_0101119 knockdown on migration and invasion were determined by detecting the expression levels of MMP-2 and MMP-9. The results demonstrated that the knockdown of hsa_circ_0101119 significantly reduced the expression levels of MMP-2 and MMP-9 in SiHa and HeLa cells (Fig. 4C).

Figure 4.

Knockdown of hsa_circ_0101119 inhibits the migration and invasion of SiHa and HeLa cells. (A) The migration of SiHa and HeLa cells was determined using a Transwell assay after transfection with si-hsa_circ_0101119 (magnification, ×100). (B) The invasion of SiHa and HeLa cells was determined using Transwell assay after transfection with si-hsa_circ_0101119 (magnification, ×100). (C) After transfection with si-hsa_circ_0101119, western blotting was performed to measure the expression levels of E-cadherin, N-cadherin, Vimentin, MMP-2 and MMP-9 in SiHa and HeLa cells. **P<0.01 vs. si-NC group. NC, negative control; si, small interfering RNA; circ, circular RNA.

The epithelial to mesenchymal transition has been reported to serve important roles in CC progression and metastasis (29). The expression level of E-cadherin in SiHa and HeLa cells was markedly increased after knockdown of hsa_circ_0101119, but the expression levels of N-cadherin and Vimentin were significantly decreased (Fig. 4C). These results indicated that the knockdown of hsa_circ_0101119 could inhibit the migration and invasion of SiHa and HeLa cells.

hsa_circ_0101119 recruits EIF4A3 to inhibit TCEAL6 expression in CC

The bioinformatics analysis revealed that hsa_circ_0101119 can bind to EIF4A3 (RF Classifier: 0.80; SVM Classifier: 0.77) (Fig. 5A). Using RIP and RNA pull down assays, it was also identified that hsa_circ_0101119 could bind to EIF4A3 in SiHa and HeLa cells (Fig. 5B and C). To examine whether has_circ_0101119 could directly regulate TCEAL6 after binding with EIF4A3, bioinformatics analysis was conducted to predict the binding abundance between EIF4A3 and TCEAL6. The scores for RF Classifier and SVM Classifier were 0.80 and 0.60, respectively (Fig. 5D), suggesting that EIF4A3 likely binds to TCEAL6. Moreover, the results of RIP assay verified this binding relationship (Fig. 5E).

Figure 5.

hsa_circ_0101119 recruits EIF4A3 to inhibit TCEAL6 expression in CC. (A) Bioinformatics was used to predict the interaction probabilities of the RNA-binding protein EIF4A3 with hsa_circ_0101119. Predictions with probabilities >0.5 were considered ‘positive’, suggesting that the corresponding RNA and protein are likely to interact. (B) RIP assay using anti-EIF4A3 showed that EIF4A3 precipitated hsa_circ_0101119 in SiHa and HeLa cell lysates. (C) Pull down assay indicated that biotin-labeled hsa_circ_0101119 interacted with EIF4A3. (D) Bioinformatics was used to predict the interaction probabilities of EIF4A3 with TCEAL6. (E) RIP assay using anti-EIF4A3 showed that EIF4A3 precipitated TCEAL6 in SiHa and HeLa cell lysates. (F) Expression levels of EIF4A3 and TCEAL6 were detected via RT-qPCR in CC cell lines (C-33A, SiHa, CaSki and HeLa) and a normal human cervical epithelial cell line, HcerEpic. (G) Expression levels of EIF4A3 and TCEAL6 in CC tissues and normal tissues, according to the analysis of TCGA. (H) Correlation between EIF4A3 and TCEAL6 in CC samples from TCGA. (I) After transfection with sh-EIF4A3, RT-qPCR was used to detect EIF4A3 expression in SiHa and HeLa cells. (J) After transfection with sh-EIF4A3, western blotting was performed to detect the expression level of TCEAL6 in SiHa and HeLa cells. (K) After co-transfection with si-hsa_circ_0101119 and sh-EIF4A3, western blotting was performed to measure the expression level of TCEAL6 in SiHa and HeLa cells. (L) A proposed model whereby hsa_circ_0101119 sequesters EIF4A3 away from TCEAL6 mRNA, in turn suppressing TCEAL6 mRNA translation. **P<0.01 vs. IgG group (B and E); *P<0.05, **P<0.01 vs. HcerEpic cells group (F); *P<0.05 vs. normal tissues group (G); **P<0.01, vs. sh-NC group (I and J); **P<0.01 vs. sh-NC group, ##P<0.01, vs. si-hsa_circ group. (K) RIP, RNA immunoprecipitation; RT-qPCR, reverse transcription-quantitative PCR; TCGA, The Cancer Genome Atlas; sh, short hairpin RNA; NC, negative control; si, small interfering RNA; circ, circular RNA; EIF4A3, eukaryotic initiation factor 4A-3; TCEAL6, transcription elongation factor A-like 6; T, tumor; N, normal; CC, cervical cancer.

It was found that EIF4A3 was highly expressed and TCEAL6 was lowly expressed in CC cells (Fig. 5F). Similarly, the analysis of The Cancer Genome Atlas (TCGA) dataset also indicated that EIF4A3 expression was significantly upregulated and TCEAL6 expression was downregulated in CC tissues (Fig. 5G). Moreover, the analysis of TCGA dataset revealed that EIF4A3 expression was negatively correlated with TCEAL6 expression in CC tissues (Fig. 5H).

As shown in Fig. 5I, RT-qPCR was used to verify the transfection efficiency of EIF4A3 knockdown. The results demonstrated that knockdown of EIF4A3 significantly increased the expression level of TCEAL6 in SiHa and HeLa cells (Fig. 5J), further suggesting that EIF4A3 expression was negatively associated with TCEAL6 expression in CC. In addition, it was found that knockdown of hsa_circ_0101119 significantly elevated TCEAL6 expression in SiHa and HeLa cells (Fig. 5K). Compared with the si-hsa_circ group, the co-transfection of si-hsa_circ and sh-EIF4A3 significantly increased the expression level of TCEAL6 in SiHa and HeLa cells (Fig. 5K). The schematic diagram depicts that hsa_circ_0101119 regulates TCEAL6 expression by enhancing the binding of EIF4A3 to TCEAL6 mRNA, which inhibits TCEAL6 translation (Fig. 5L).

TCEAL6 overexpression inhibits the proliferation, migration and invasion, and promotes the apoptosis of SiHa and HeLa cells

As shown in Fig. 6A, RT-qPCR was used to verify the transfection efficiency of TCEAL6 overexpression. To verify the effect of TCEAL6 overexpression, colony formation assays (Fig. 6B), flow cytometry (Fig. 6C) and Transwell assays (Fig. 6D and E) were performed. The results indicated that TCEAL6 overexpression significantly inhibited the proliferation, migration and invasion, while it promoted the apoptosis of SiHa and HeLa cells (Fig. 6B-E).

Figure 6.

TCEAL6 overexpression inhibits proliferation, migration and invasion, and promotes apoptosis in SiHa and HeLa cells. (A) Expression level of TCEAL6 was detected via reverse transcription-quantitative PCR in transfected SiHa and HeLa cells. (B) After transfection with pcDNA-TCEAL6, colony numbers of SiHa and HeLa cells were detected using a colony formation assay (magnification, ×100). (C) After transfection with pcDNA-TCEAL6, flow cytometry was used to detect the apoptosis of SiHa and HeLa cells. After transfection with pcDNA-TCEAL6, the (D) migration and (E) invasion of SiHa and HeLa cells was determined using a Transwell assay (magnification, ×100). **P<0.01 vs. pcDNA-NC group. NC, negative control; TCEAL6, transcription elongation factor A-like 6.

Knockdown of TCEAL6 reverses the effects of hsa_circ_0101119 knockdown on the proliferation, apoptosis, migration and invasion of HeLa cells

As presented in Fig. 7A, the transfection efficiency of sh-TCEAL6 was assessed via RT-qPCR. The results demonstrated that the proliferation, cell clones, migration and invasion of HeLa cells were significantly reduced in the si-hsa_circ + sh-NC group compared with the si-NC + sh-NC or si-hsa_circ + sh-TCEAL6 groups, while apoptosis was significantly elevated (Fig. 7B-E). In addition, compared with the si-NC + sh-NC or si-hsa_circ + sh-TCEAL6 groups, the proliferation, cell clones, migration and invasion of HeLa cells were significantly increased in the si-NC + sh-TCEAL6 group, but apoptosis was significantly decreased (Fig. 7B-E). These results indicated that the knockdown of TCEAL6 could reverse the effects of hsa_circ_0101119 knockdown on the proliferation, apoptosis, migration and invasion in HeLa cells.

Figure 7.

Knockdown of TCEAL6 reverses the effects of silencing hsa_circ_0101119 on the proliferation, apoptosis and migration and invasion in HeLa cells. (A) After transfection, the expression level of TCEAL6 was detected via reverse transcription-quantitative PCR in HeLa cells. (B) After co-transfection with si-hsa_circ_0101119 and sh-TCEAL6, HeLa cell proliferation was determined using an EdU assay (magnification, ×200). (C) After co-transfection with si-hsa_circ_0101119 and sh-TCEAL6, the colony numbers of HeLa cells were detected with a colony formation assay (magnification, ×100). (D) After co-transfection with si-hsa_circ_0101119 and sh-TCEAL6, flow cytometry was used to detect HeLa cell apoptosis. (E) After co-transfection with si-hsa_circ_0101119 and sh-TCEAL6, the migration and invasion of HeLa cells were determined using a Transwell assay (magnification, ×100). **P<0.01 vs. si-NC + sh-NC group; ##P<0.01 vs. si-hsa_circ + sh-NC; &&P<0.01 vs. si-NC + sh-TCEAL6 group. sh, short hairpin RNA; NC, negative control; si, small interfering RNA; circ, circular RNA; TCEAL6, transcription elongation factor A-like 6; EdU, 5-ethynyl-20-deoxyuridine; circ, circular RNA.

Discussion

According to public reports, >450,000 patients are diagnosed with CC and ~266,000 individuals die of CC every year (30,31). Although different strategies have been used for the treatment of CC, CC remains a health problem. Therefore, it is essential to identify effective therapeutic targets and discover the underlying molecular mechanisms in CC progression. In the present study, it was demonstrated that hsa_circ_0101119 could facilitate cell proliferation, migration and invasion, and suppress apoptosis in CC via an interaction with EIF4A3 to inhibit TCEAL6 expression.

Accumulating evidence has indicated that the dysregulation of circRNAs is associated with the occurrence and development of malignancies (32,33). For example, the expression level of circ_102231 is upregulated in lung adenocarcinoma tissues (34). Zhang et al (35) reported that hsa_circ_0023404 was highly expressed and exerted an oncogenic role in CC. By analyzing the GSE102686 chip, the present study identified that the expression level of hsa_circ_0101119 was significantly upregulated in CC tissues compared with that in normal tissues. In addition, it was demonstrated that hsa_circ_0101119 expression was markedly increased in CC cells, which was in line with a previous study by Wang et al (11). It has also been revealed that circRNAs can serve a critical role in different biological processes, including proliferation, survival, apoptosis and metastasis (36). For instance, circ-MYB proto-oncogene like 2 is reported to facilitate the proliferation and invasion of CC cells (37). Moreover, Chen et al (38) suggested that circRNA myosin light chain kinase could accelerate cell proliferation and repress apoptosis via the upregulation of Ras homolog, mTORC1 binding and the activation of the mTOR pathway in CC. Wang et al (39) also revealed that the overexpression of hsa_circ_0001038 could promote cell proliferation and invasion by regulating cyclin-M3 and metastasis-associated in colon cancer 1 expression in CC. In the present study, it was demonstrated that the knockdown of hsa_circ_0101119 could significantly inhibit the proliferation, migration and invasion, as well as facilitate the apoptosis of SiHa and HeLa cells.

Besides miRNAs, circRNAs can bind RBPs, modulate their availability in the cells and affect the post-transcriptional fates of RBP-interacting mRNAs (5,40). The RBP EIF4A3 is reported to be an important regulator of post-transcriptional regulation processes, including mRNA splicing, transport, translation and surveillance (18). EIF4A3 has been shown to be upregulated and identified as a diagnostic marker in some cancer types, including breast cancer and lung cancer (18). Moreover, repression of EIF4A3 could affect the expression levels of transcripts associated with the cell cycle in cancer cells (41). In the recent years, numerous studies have shown that circRNAs serve vital roles in the development of cancer via binding EIF4A3. For example, circ_septin9 could significantly promote proliferation, migration and invasion, as well as inhibit apoptosis and autophagy in triple-negative breast cancer cells via binding EIF4A3 (42). Xu et al (43) also reported that circ_chromosome segregation 1 can inhibit colorectal cancer cell proliferation by binding to EIF4A3. Furthermore, circ_nectin cell adhesion molecule 3 was found to facilitate the proliferation of gastric cancer cells by combining with EIF4A3 (44). Using bioinformatics analysis, the current study predicted that hsa_circ_0101119 could bind to EIF4A3, and this binding of hsa_circ_0101119 to EIF4A3 was confirmed using RIP and RNA pull down assays.

Accumulating evidence has suggested that RBPs can conduct their roles by interacting with different types of target RNAs and forming ribonucleoprotein complexes (45,46). The current bioinformatics analysis also identified a high binding abundance between EIF4A3 and TCEAL6. In the present study, it was demonstrated that EIF4A3 significantly negatively regulated TCEAL6 in CC cells. Biewenga et al (25) reported that TCEAL6 was lowly expressed in the early stage of CC and could serve as a potential biomarker for CC, which was confirmed by the current experiments. Moreover, the present study identified that hsa_circ_0101119 could recruit EIF4A3 to inhibit TCEAL6 expression in CC, as determined via bioinformatics analysis, RIP assay, pull down assay, RT-qPCR and western blotting. To confirm this conclusion, the effects of co-transfection of si-hsa_circ_0101119 and sh-TCEAL6 on proliferation, apoptosis, migration and invasion were detected in HeLa cells. The data revealed that the knockdown of TCEAL6 could reverse the effects of silencing hsa_circ_0101119 on the proliferation, apoptosis, migration and invasion of HeLa cells. Of course, further studies are required to identify the effect of hsa_circ_0101119 and its underlying molecular mechanisms in CC in vivo.

In conclusion, the present study identified an oncogenic role of hsa_circ_0101119 in CC progression. It was demonstrated that hsa_circ_0101119 could facilitate cell proliferation, migration and invasion, and suppress apoptosis in CC via an interaction with EIF4A3 to inhibit TCEAL6 expression. These findings suggested that hsa_circ_0101119 may be an effective therapeutic target for CC treatment.