Literature DB >> 34272708

Case Study 1: Practical Considerations with Experimental Design and Interpretation.

John T Barr1, Otito F Iwuchukwu2.   

Abstract

This chapter deals with practical considerations on key issues such as choosing an enzyme source, determining linear conditions, and choosing appropriate substrate and organic solvent concentrations. Practical solutions for working with limited resources and carrying out inhibition experiments are also addressed. Thus, after reading this chapter, the novice reader should have a better idea of how to design, develop, and interpret basic experiments using drug metabolism enzymes.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords:  Drug-metabolizing enzymes; Inhibition constants; Linearity; Metabolite; Organic solvent; Standard

Year:  2021        PMID： 34272708     DOI： 10.1007/978-1-0716-1554-6_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Effect of common organic solvents on in vitro cytochrome P450-mediated metabolic activities in human liver microsomes.

Authors:  N Chauret; A Gauthier; D A Nicoll-Griffith
Journal:  Drug Metab Dispos       Date:  1998-01       Impact factor: 3.922

2.  Effects of organic solvents on the activities of cytochrome P450 isoforms, UDP-dependent glucuronyl transferase, and phenol sulfotransferase in human hepatocytes.

Authors:  J Easterbrook; C Lu; Y Sakai; A P Li
Journal:  Drug Metab Dispos       Date:  2001-02       Impact factor: 3.922

3.  Effects of freezing, thawing, and storage of human liver samples on the microsomal contents and activities of cytochrome P450 enzymes.

Authors:  H Yamazaki; K Inoue; C G Turvy; F P Guengerich; T Shimada
Journal:  Drug Metab Dispos       Date:  1997-02       Impact factor: 3.922

4.  Effect of methanol, ethanol, dimethyl sulfoxide, and acetonitrile on in vitro activities of cDNA-expressed human cytochromes P-450.

Authors:  W F Busby; J M Ackermann; C L Crespi
Journal:  Drug Metab Dispos       Date:  1999-02       Impact factor: 3.922
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.