Literature DB >> 34270010

Brassica rapa L. ssp. chinensis Isolated Microspore Culture Protocol.

Daria Shumilina1, Elena Kozar2, Olga Chichvarina2, Ksenia Korottseva2, Elena Domblides2.   

Abstract

Culture of isolated microspores is a widely used method to obtain haploid and doubled haploid plants for many crop species. This protocol describes the steps necessary to obtain a large number of microspore derived embryos for pakchoi (Brassica rapa L. ssp. chinensis) and zicaitai (Brassica rapa L. ssp. сhinensis Hanelt var. purpuraria Kitam).
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  24-Epibrassinolide; Brassica rapa ssp. chinensis; Doubled haploid; Microspore culture

Year:  2021        PMID： 34270010     DOI： 10.1007/978-1-0716-1335-1_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  Differential staining of aborted and nonaborted pollen.

Authors:  M P Alexander
Journal:  Stain Technol       Date:  1969-05

2.  A high-density genetic map developed by specific-locus amplified fragment (SLAF) sequencing and identification of a locus controlling anthocyanin pigmentation in stalk of Zicaitai (Brassica rapa L. ssp. chinensis var. purpurea).

Authors:  Gui-Hua Li; Han-Cai Chen; Jia-Li Liu; Wen-Long Luo; Da-Sen Xie; Shao-Bo Luo; Ting-Quan Wu; Waheed Akram; Yu-Juan Zhong
Journal:  BMC Genomics       Date:  2019-05-07       Impact factor: 3.969
  2 in total

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