Myocarditis Induced by Immune Checkpoint Inhibitors: Mechanisms and Therapeutic Prospects.

Under physiological conditions, immune checkpoint molecules downregulate the activation and effector function of myocardial antigen-reactive T cells through an immunosuppressive pathway, thus enabling myocardial T cells to maintain immune homeostasis under the action of central and peripheral tolerance mechanisms. The PD-1/PD-L1 signalling pathway is particularly important for limiting the ability of T cells to attack the heart. Immune checkpoint inhibitors (ICIs) specifically block this PD-1/PD-L1-mediated restriction of T cell activation and other immunosuppressive pathways by targeting immune checkpoints. In recent years, with the wide use of ICIs in cancer treatment, even though the incidence of immunomyocarditis is low, it has attracted increasing attention because of its complex clinical symptoms, rapid progression of disease and high mortality rates. The pathogenesis, genetic susceptibility factors and predictive biomarkers of immunomyocarditis still need to be understood, and multidisciplinary cooperation in the clinical treatment of this complication is necessary.

Introduction

Remarkable clinical efficacy has been achieved with programmed death 1 (PD-1), programmed death ligand-1 (PD-L1), and cytotoxic T-lymphocyte associated protein-4 (CTLA-4) blockades and these treatments have survival benefits when applied to treat several cancers, including head and neck squamous cell carcinoma, urothelial cancer, Hodgkin’s lymphoma, non-Hodgkin’s lymphoma, gastric cancer, adenocarcinoma of the gastroesophageal junction, liver cancer, colorectal cancer, Merkel cell carcinoma, triple negative breast cancer, and squamous cell carcinoma.1–10 Since the first ICI targeting CTLA-4 (ipilimumab) was approved as a post-first-line treatment for unresectable or metastatic melanoma by the FDA in 2011,11 the FDA has approved seven ICIs, comprising anti-PD-1 (pembrolizumab, nivolumab), anti-PD-L1 (atezolizumab, durvalumab, avelumab), and anti-CTLA-4 (ipilimumab, tremelimumab) drugs.

Due to the clonality between normal tissues or organs and tumour cell surface antigens, although ICIs can restore the tumour-killing function of cytotoxic T cells, ICIs inevitably attenuate the self-tolerance of the body to autoimmune cells and lead to multiple systemic immune-related adverse events (irAEs). The related irAEs reported in studies include rash, colitis, pneumonitis, hepatitis, neurologic, nephritis, haematologic, infection, thrombosis, electrolyte imbalance, and multiorgan failure.12,13 Immunomyocarditis is characterized by hidden symptoms and a high mortality rate. Therefore, though immunomyocarditis is rare, this ICI-mediated side effect is important to consider in clinical work.14 The incidence of immunomyocarditis was 0.06–1% in cancer patients enrolled in clinical trials receiving ICIs.15 Furthermore, Johnson et al demonstrated a significant difference in the immunomyocarditis incidence rate between patients treated with nivolumab (0.06%) and those treated with nivolumab plus ipilimumab (0.27%).16

Immunomyocarditis commonly presents as heart failure, arrhythmia, myocardial pericarditis, cardiomyopathy, myocardial fibrosis, cardiogenic shock, and cardiogenic death.17–21 The lack of specific symptoms in the early stage of immunomyocarditis and the lack of regular cardiovascular examinations in the course of disease management lead to the underestimation of the incidence of immunomyocarditis and the severity of the disease. At present, no guidelines exist for the diagnosis and treatment of immunomyocarditis.15

In this review, we analyse the mechanism and breakthroughs of ICIs in the treatment of tumours, the pathogenesis of immunomyocarditis, the potential risk factors for immunomyocarditis, the consensus of diagnosis and management of immunomyocarditis, and the exploration of treatment models that may reduce the risk of immunomyocarditis.

Immune Checkpoint Inhibitors in Cancers

CTLA-4 and PD-1/PD-L1

Breakthroughs have been made in clinical trials that evaluated ICIs that specifically block the CTLA-4 and PD-1/PD-L1 signalling; thus, these were the first immunotherapy drugs to be used for cancer.

CTLA-4 is a transmembrane receptor expressed on T cells and shares B7 molecular ligands with CD28. The competitive binding of CTLA-4 molecule on the T cell surface to the B7 molecule on APC cell surfaces leads to the activation of negative costimulatory molecules, which in turn inhibits the activation of T cells (Figure 1).22–26 PD-L1 is mainly expressed on the surface of a variety of solid tumour cells.27–33 The PD-1/PD-L1 signal pathway is formed by PD-1, which is highly expressed on T cell surface, and the PD-L1 or PD-L2 molecule, which is expressed on the tumour cell surface and transmits inhibitory signal to effector T cells, inhibits T cell proliferation and specific killing of tumour cells, and allows tumour cells to escape the immune system.34–36

Figure 1

Mechanism of PD-1 and CTLA-4 in inhibiting T cell activation.

The FDA has approved two kinds of ICIs blocking the CTLA-4/B7 pathway, which include anti-CTLA-4 drugs (ipilimumab, tremelimumab) (Table 1). Ipilimumab is the first drug in history to significantly improve the OS rate of patients with advanced metastatic melanoma. According to the results of the DETERMINE clinical trial, tremelimumab has been approved as a second-line or third-line treatment for relapsed malignant mesothelioma.38

Table 1

FDA-Approved 7 Kinds of Immune Checkpoint Inhibitors

Target Pathway	Agent	IgG Class	Company	Approved Cancer	References
CTLA-4	Ipilimumab	Human IgG1	Bristol-Meyers Squibb	Melanoma	[37]
	Tremelimumab	Human IgG2	AstraZeneca	Malignant mesothelioma	[38]
PD-1	Pembrolizumab	Humanized IgG4	Merck	Melanoma	[39]
				Lung cancer	[40,41]
				Head and neck cancer	[42]
	Nivolumab	Human IgG4	Bristol-Meyers Squibb/Ono	Melanoma	[11]
			Colorectal cancer	Non-small cell lung cancer	[43,44]
				Kidney cancer	[45]
				Hodgkin’s lymphoma	[46]
				Squamous cell carcinoma of the head and neck	[47]
				Urothelial carcinoma	[48]
				Hepatocellular carcinoma	[49]
PD-L1	Atezolizumab	Humanized IgG1k	Roche/Genentech	Urothelial cancer	[50]
				Non-small cell lung cancer	[51]
	Durvalumab	Human IgG1k	Medimmune/AstraZeneca	Urothelial cancer	[53]
				Non-small cell lung cancer	[54]
				Small cell lung cancer	[55]
	Avelumab	Human IgG1	Merck Serono/Pfizer	Meckel cell carcinoma	[56]
				Urothelial cancer	[57]

The FDA has approved five kinds of ICIs blocking the PD-1/PD-L1 pathway, which include anti-PD-1 (pembrolizumab, nivolumab) and anti-PD-L1 (atezolizumab, durvalumab, avelumab) drugs (Table 1). Pembrolizumab has been approved as a first-line treatment for advanced non-small cell lung cancer (NSCLC) positive for PD-L1 expression (>50%) (Keynote-024).40,41 Nivolumab has been approved as a second-line treatment for advanced squamous NSCLC (CheckMate-017) and advanced non-squamous NSCLC (CheckMate-057).43,44 According to the results of the POPLAR and OAK clinical trials, atezolizumab has been approved as a second-line treatment for advanced NSCLC.5,51,52 Durvalumab was approved as the standard treatment for locally advanced or metastatic urothelial cancer in 2017.53 Subsequently, avelumab has been approved for the treatment of metastatic Merkel cell carcinoma.56 Additionally, combining anti-PD-1/PD-L1 and anti-CTLA-4 antibodies could further strengthen the antitumour efficacy of the drugs and patient survival outcomes.58–60

Other Targets

Recently, many studies have proposed that other immune checkpoint molecules also play an important role in tumour progression, but they are still in the research stage, and there are no drugs approved by the FDA for clinical treatment at present.

TIM-3

Similar to PD-1 and CTLA-4, T cell immunoglobulin domain and mucin domain-3 (TIM-3) is also an inhibitory receptor expressed on the surface of CD4+ T helper 1 and CD8+ T cytotoxic cells that results in T cell exhaustion.61–63 Sakuishi et al found that CD8+ T cells exhibited an exhaustion status with a PD-1+/TIM3+ phenotype and that anti-TIM-3 could reverse the therapy resistance induced by anti-PD-1.64

LAG-3

Lymphocyte activation gene-3 (LAG-3) is another immune checkpoint inhibitor that is expressed on the surface of T cells, B cells, NK cells, and DCs. LAG-3 could inhibit the killing efficacy of T cells and promote the intrinsic inhibitory function of Tregs.65 Anti-LAG-3 synergises with anti-PD-1 and can restore CD8+ T cell function and reduce tumour immune escape.66,67

TIGIT

T cell immunoreceptors with Ig and ITIM domains (TIGIT) are widely expressed on human tumour-infiltrating T cells. TIGIT synergises with inhibitory receptors such as PD-1, TIM-3, and LAG-3, and leads to exhaustion of infiltrating T cells and weakens the tumour killing ability of cytotoxic CD8+ T cells. Robert et al demonstrated that co-blockade of TIGIT and PD-L1 significantly enhanced CD8+ T cell function and reduced tumour volume.68

Inhibitory Pathways in Myocardial Immune Homeostasis

PD-1, a receptor of the IgM superfamily, is an immunosuppressive receptor that is usually expressed on the surface of activated T cells, B cells and myeloid cells. It negatively regulates the signal transduction of T cell antigen receptors by interacting with the specific ligand PD-L1 and is thought to play a role in maintaining self-tolerance in the immune system.69 PD-L1 is expressed by antigen-presenting cells, including peripheral blood monocytes and activated dendritic cells.70 In 1998, to analyze the role of PD-1 molecules in the immune response, Nishimura et al selected PD-1 gene-deficient mice as experimental subjects. The results showed that PD-1 signalling molecules inhibit the proliferation and differentiation of B lymphocytes. Similar to CTLA-4 signalling molecules, PD-1 molecules also weaken the immune response.71 In addition, Freeman et al found that the expression level of PD-L1 mRNA in mouse heart tissue was very high.70 Rodig et al confirmed that IFN-γ could induce mouse cardiac endothelial cells cultured in vitro to express PD-L1, and that this significantly weakened the IFN-γ-mediated class I MHC restriction antigen presentation to cytotoxic T lymphocytes through the PD-1/PD-L1 signalling pathway. Furthermore, IFN-γ could enhance the resistance of mouse cardiac endothelial cells to cytotoxic T lymphocyte attack and maintain the immune homeostasis of cardiac endothelial cells.72 To further confirm the role of PD-L1 in maintaining the immune homeostasis of cardiomyocytes, Grabie et al established a mouse model of cytotoxic T lymphocyte-mediated immunomyocarditis and used PD-L1/L2 gene-deficient mice and PD-L1/L2-specific antibodies to study the effect of the PD-L1 signalling pathway on the myocardium. The results showed that blocking PD-L1 could increase the microabscess formed by leukocytes but did not affect the recruitment of cytotoxic T lymphocytes. This eventually lead to the transformation of immunomyocarditis into fatal heart disease.73 The above studies reveal that the PD-L1 signalling pathway plays a role in immune tolerance and myocardial protection mainly by limiting the effect of CTLs rather than by reducing the recruitment of CTLs to cardiomyocytes. Under normal physiological conditions, DCs continuously present cardiac antigens to naïve T cells in cardiac draining lymph nodes, making the heart tolerant to antigen-specific circulating naïve T cells. When cardiac infection or peripheral tolerance genetic variation occurs, specific naïve T cells are activated, differentiated and expanded into effector T cells. IFN-γ produced by both effector T cells and tissues induces cardiomyocytes to express PD-L1, and the PD-1/PD-L1 signalling pathway cooperates with other regulatory mechanisms to protect the heart from T cell attack and maintain myocardial immune equilibrium.74,75

Mechanisms of Immunomyocarditis Arising as a Consequence of ICI Treatment

ICIs can relieve the PD-1/PD-L1-mediated inhibition of tumour killing T cells and other immunosuppressive pathways, enhance the function of T cells, and show a good effect in the treatment of many kinds of cancer. Because cardiomyocytes protect themselves from T cell attacks and maintain immune tolerance through high expression of PD-L1, it is necessary to avoid immunomyocarditis that arises due to immune tolerance with ICI therapy for cancer as immunomyocarditis is caused by the abnormal activation of T cells and subsequent damage to cardiomyocytes.16

In an animal model study, Waterhouse et al studied CTLA-4 gene-deficient mice and found that infiltrating T cells were significantly activated and neutrophils and macrophages infiltrated the heart, which eventually led to myocardial fibrosis. One month later, CTLA-4 gene-deficient mice died of myocardial infarction.26 Nishimura et al found that five weeks after PD-1 knockout, the mice died of severe dilated cardiomyopathy, ventricular systolic dysfunction, and congestive heart failure.76 Another study also revealed the role of PD-1 in maintaining myocardial immune tolerance. PD-1 knockout mice showed a severe inflammatory response, increased myocardial injury, and significantly increased inflammatory cell infiltration in the myocardium.77 In addition, PD-1 knockout mice produce autoantibodies against cardiac myosin, which leads to a significant increase in CD4+ T, CD8+ T and myeloid cell infiltration in the myocardium and eventually develops into fatal immunomyocarditis.78

At present, the mechanism of immunomyocarditis is not clear, and the possible mechanisms are as follows. First, immune checkpoint molecules act as new antigens to stimulate the T cell response. Studies have shown that the expression of PD-L1 molecules is significantly upregulated not only in a variety of tumour cells but also in normal tissues, including cardiac endothelial cells,79 respiratory epithelial cells,80 renal epithelial cells,81 islet cells82 and skeletal muscle cells.83 One study revealed that two patients developed fulminant immunomyocarditis after treatment with anti-PD-1 plus anti-CTLA-4. Johnson et al analyzed myocardial tissue from these patients and found that PD-L1 was highly expressed in CD8+ T cells infiltrating the myocardium and damaged cardiomyocytes, but no PD-L1 expression was found in skeletal muscle and tumour tissue.16 The mechanism of the upregulation of PD-L1 expression in cardiomyocytes after ICI therapy is not clear. A possible explanation is that PD-L1 activates T cells as a new antigen, the T cells cannot directly recognize the antigen, and the TCR on the surface of T cells recognizes the antigen peptide-MHC molecular complex on the surface of antigen-presenting cells to complete the process of antigen recognition. We can detect the TCR-mediated clonal expansion of infiltrating T cells to determine whether PD-L1 expressed by cardiac endothelial cells can stimulate the T cell response as a new antigen, which leads to immunomyocarditis. Another possibility is that damaged cardiomyocytes inhibit T cell function activated by ICIs by upregulating PD-L1 expression, thus avoiding further aggravation of immunomyocarditis.

Second, ICIs enhance the ability of T cells to recognize and bind antigens. A variety of antigen molecules are expressed in tumour and heart tissues, and cardiac antigens express tissue antigens that are homologous to tumour tissues.84,85 For instance, Johnson et al analyzed tumour tissue sections from patients who died from immunomyocarditis and found that the tumour tissue highly expressed muscle-specific antigens (desmin and troponin), which are homologous to myocardial tissue.16 Therefore, when T cells recognize tumour antigens, they can circulate and recognize homologous tissue antigens in the heart and promote cardiac inflammatory infiltration and induce off-target effects and the occurrence of immunomyocarditis.83 Johnson et al conducted an immunohistochemical analysis of the tissues of two patients with fulminant immunomyocarditis and found that there were a large number of CD4+ and CD8+ T cells infiltrating the myocardium, skeletal muscle and tumour tissues. Researchers sequenced the TCR expressed by infiltrating T cells and found that T cells were highly clonal and had expanded in these tissues, indicating that T cells recognize antigens in tumour tissues and off-target tissues after activation by ICIs. This in turn leads to the occurrence of immunomyocarditis. To further clarify the exact T cell subsets activated by ICIs, Yamaguchi et al analyzed peripheral blood mononuclear cells from patients with multiple myeloma by flow cytometry and found that CD4+ and CD8+ T cells increased after treatment with ICIs.86 In addition, a number of studies have shown that CD4+ and CD8+ T cells are involved in the pathological process of immunomyocarditis. Th2 cell subsets and Th17 subsets of CD4+ T cells regulate cardiac inflammation by secreting inflammatory mediators and promote the occurrence of immunomyocarditis.87–89 CD8+ T cells play a cytotoxic role in promoting the occurrence and progression of immunomyocarditis.90,91

Third, the infiltration of inflammatory factors into off-target organs promotes the progression of immunomyocarditis. Recent studies have shown that a patient with recurrent metastatic melanoma developed acute renal interstitial glomerulonephritis after receiving anti-CTLA-4 combined with anti-PD-1 therapy. Through analysis, they found that the levels of serum proinflammatory cytokines, such as IL-1Ra, CXCL10 and TNF-α, were significantly increased (Figure 2).16 However, there is still a lack of clinical evidence for the function of circulating cytokines in patients with immunomyocarditis. The involvement of inflammatory cytokines in the development of immunomyocarditis needs to be confirmed in further clinical studies.

Figure 2

Anti-PD-1 therapy breaks the peripheral immune tolerance by unleashing effective T cells.

Autopsy analysis showed that the infiltration of CD3+T cells, CD4+T cells and CD8+T cells in the myocardial tissue of patients with immunomyocarditis was significantly increased.92 Professor Hardy found that CD3+T cells and CD68+ macrophages mainly infiltrated into myocardial tissue, while the number of CD4+T cells in the CD3+T cell population was significantly higher than the number of CD8+T cells.93 The myocardial biopsy of another melanoma patient treated with Nivo monoclonal antibody showed infiltration of CD8+ and PD-1- T cells.94 Sobol et al analyzed the myocardial biopsy samples of 6 patients with immunomyocarditis and found that most immunomyocarditis was multifocal/diffuse lesions, in which CD8+ and PD-1+ T cell infiltration was increased, and confirmed the expression of PD-L1-mediated immune activity in the myocardial lesion area.95 Champion et al divided 10 patients with immunomyocarditis into two subgroups, high-grade immunomyocarditis (> 50 CD3+ cells/hpf) and low-grade immunomyocarditis (≤ 50 CD3+ cells/hpf). Their study revealed that the density of CD3+ T cells, CD8+ T cells and CD68+ macrophages in patients with high-grade immunomyocarditis was higher, the time to disease onset was shorter, the clinical symptoms were more severe, and the survival rate was lower.96

Clinical Characteristics and Therapeutic Implications of Immunomyocarditis

The timing of immunomyocarditis varies. Hardy et al reported an 81-year-old patient with recurrent renal cell carcinoma who developed third-degree heart failure two days after receiving anti-PD-1 combined with anti-CTLA-4 and died of severe immunomyocarditis on the third day.93 Some studies have shown that immunomyocarditis is an early cardiotoxicity after treatment with anti-PD-1 combined with anti-CTLA-4, and the median time for diagnosis from the first dose of ICI is approximately 15 days,12,16 while after the first dose of anti-PD-1 or anti-CTLA-4, the median time for diagnosis of immunomyocarditis is 40 days.12 In addition, a retrospective study showed that 76% of immunomyocarditis occurrences arose within 6 weeks after the first dose of ICI, with a median time of 27 days (5–155 days).97 Compared with ICI-mediated early immunomyocarditis (occurring in the first 90 days of treatment), ICI-mediated late immunomyocarditis (after the first 90 days of treatment) is more likely to develop heart failure.98 Although the incidence of immunomyocarditis is not high, the mortality rate of immunomyocarditis is the highest of all ICI-mediated side effects. Wang et al retrospectively analyzed 613 cases of fatal ICI-mediated side effects reported from 2009 to 2018 and found that 333 cases were caused by anti-PD-1/PD-L1, and 27 patients (8%) died from immunomyocarditis. In addition, 87 cases were caused by anti-PD-1 combined with anti-CTLA-4, and 22 patients died from immunomyocarditis (25%).15 Moslehi et al found that among 101 patients with severe immunomyocarditis, the mortality rate of patients treated with anti-PD-1/PD-L1 was 36%, while that of patients treated with anti-PD-1/PD-L1 combined with anti-CTLA-4 was as high as 67%.97 In addition, patients with diabetes were more likely to develop immunomyocarditis. Therefore, we should pay considerable attention to the high mortality rate of immunomyocarditis.

Patients with immunomyocarditis have different onset times and various clinical symptoms after receiving ICI treatment. The initial symptoms of low-grade immunomyocarditis lack specificity, and most of them are common uncomfortable symptoms, such as palpitation, fatigue, and chest tightness. On the other hand, high-grade immunomyocarditis usually has serious clinical symptoms such as fulminant immunomyocarditis and a high mortality rate.96 Typical clinical symptoms of immunomyocarditis include palpitation, chest pain, fatigue, left ventricular systolic dysfunction, abnormal myocardial zymogram, arrhythmia, heart failure and myocardial infarction.14,16,19–21,99,100 For patients with suspected immunomyocarditis during ICI treatment, a cardiologist should be immediately consulted to compare baseline electrocardiogram and echocardiography with immediate electrocardiogram and echocardiography. Furthermore, cardiac biomarkers and inflammatory biomarkers should be dynamically monitored. In clinical practice, baseline electrocardiogram (ECG) and troponin tests are usually performed in patients treated with ICIs. ECG and troponin were measured 7 days after the first injection of ICIs and were also measured before each cycle of ICI treatment. If necessary, cardiac magnetic resonance imaging or myocardial biopsy should be performed to diagnose the condition (Figure 3).101 For patients with grade 1 or 2 mild immunomyocarditis, intravenous injection of methylprednisolone (1–2 mg/kg) is recommended. After improvement, the dose should be reduced once every 1–2 weeks, and the reduction process should not be shorter than 4–6 weeks. For patients with grade 3 or 4 severe immunomyocarditis, ICI therapy should be stopped permanently, and intravenous high-dose methylprednisolone (1 g/day) pulse therapy is recommended for 3–5 days. After improvement, the dose should be reduced once every 1–2 weeks until a dose of 1–2 mg/kg/day, and the reduction process can last 6–8 weeks.102 If the symptoms of patients with grade 3 or 4 immunomyocarditis are not relieved within 24 hours after taking large doses of corticosteroids, immunosuppressants such as antithymocyte globulin, infliximab or intravenous immunoglobulin should be considered, or treatment with the CTLA-4 agonist abatacept, to destroy peripheral immune cells and restore normal peripheral immune tolerance.103–107

Figure 3

Proposed algorithm for work-up and management of ICI-mediated myocarditis.

Challenges and Prospects

Differences in Tumour Immunogenicity

At present, the FDA has approved ICIs as a first-line treatment or post-first-line treatment for patients with advanced cancer, including melanoma, NSCLC, renal cell carcinoma, Hodgkin’s lymphoma, head and neck squamous cell carcinoma, urothelial carcinoma, colorectal cancer, hepatocellular carcinoma, gastric cancer, gastroesophageal junction adenocarcinoma, and Merkel cell carcinoma. According to reported studies, immunomyocarditis mostly occurs in patients with melanoma and non-small cell lung cancer, while cases of immunomyocarditis reported in other tumour types are relatively few.97 We speculated that the possible reason is that both melanoma and NSCLC are “immunogenic hot tumours”. The clonal diversity of various antigenic molecules on the cell surface easily activates the T cell response and increases the possibility of cross-immune reactions between tumour tissue and heart tissue, which leads to the occurrence of immunomyocarditis. In highly immunogenic tumours, ICI therapy is a double-edged sword that not only achieves a high response rate but also increases the possibility of immunomyocarditis. Therefore, when ICIs are used for patients with hyperimmunogenic tumours, a combination of cardioprotective agents may be a good choice.

Immunotherapy Regimens

In a retrospective study of immunomyocarditis data from 8 clinical centres, researchers found that the incidence of immunomyocarditis was higher in patients treated with anti-PD-L1 than in those treated with anti-PD-1, while the incidence of immunomyocarditis was highest in patients treated with anti-CTLA-4.18 Another retrospective study included 42 cases of immunomyocarditis, of which the highest incidence was caused by anti-PD-1 treatment (43%).14 Therefore, a study with a larger sample size is still needed to analyze which ICI is more likely to cause immunomyocarditis. At present, there is a general consensus that when anti-PD-1/PD-L1 and anti-CTLA-4 are used simultaneously, the incidence of immunomyocarditis is the highest, and the symptoms are more severe.84,102 Once severe immunomyocarditis occurs, the mortality rate of patients treated with ICIs is as high as 67%.97 Therefore, we need to constantly explore the efficacy of new ICIs or activators in cancer treatment, and we expect to achieve lower toxicity and side effects.85

Candidate Biomarkers

As important cardiac biomarkers, troponin and natriuretic peptide play a critical role in the diagnosis of conventional heart disease.108 It has been reported that the expression of troponin is significantly increased in patients with immunomyocarditis. Because patients often also have myositis, additional monitoring of creatine kinase, myoglobin and lactate dehydrogenase is necessary.109 In addition, Tsuruda et al monitored the changes in serum concentrations of IL-6, IL-8 and GM-CSF in 3 patients with immunomyocarditis at different stages of the disease. They found that IL-8 levels were significantly increased in 3 patients with immunomyocarditis, suggesting that IL-8 may be related to the severity of immunomyocarditis. However, this result still needs to be further verified by a large clinical trial.110 To date, there is still a lack of research on biomarkers for the early prediction of immunomyocarditis.111 We need large-scale clinical studies to explore effective biomarkers that can predict immunomyocarditis early to maximize the killing of tumour cells on the basis of the safe use of ICIs.

Conclusions

Immunomyocarditis is a special immunotherapy-mediated toxicity of autoimmune T cells activated by ICIs that enables the myocardium to overcome peripheral immune tolerance through a series of immune activation effects and inflammatory cytokines. The clinical characteristics of immunomyocarditis are different from those caused by common myocarditis and cytotoxic drugs. Immunomyocarditis usually occurs within 6 weeks after initial treatment with ICIs. The course of the disease progresses rapidly, and the clinical symptoms are serious. In the course of treatment, high-dose methylprednisolone pulse therapy is necessary. However, due to the low incidence of immunomyocarditis, there is still a lack of clinical research on its pathogenesis and the use of biomarkers to predict its occurrence. With the wide use of ICIs and the high mortality of immunomyocarditis, large-scale clinical studies on early predictive biomarkers in immunomyocarditis are needed.