The Role and Mechanism of MALAT1 Long Non-Coding RNA in the Diagnosis and Treatment of Head and Neck Squamous Cell Carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is the most common malignant tumor of the head and neck. HNSCC mainly affects the oral cavity and the laryngeal, laryngopharyngeal, and oropharyngeal tracts. The high incidence, hidden onset, low survival rate, and unsatisfactory effects of treatment effect underscore the importance of identify the mechanisms of HNSCC occurrence and development. Although there is a very urgent need for early diagnosis and treatment, there are currently no reliable early HNSCC diagnosis biomarkers or effective treatment targets. Long non-coding RNA (lncRNA) is widely involved in biological processes, especially as a key regulator of tumorigenesis and development. Lung adenocarcinoma metastasis-associated transcript 1 (MALAT1) is an important member of the lncRNA family that can regulate the occurrence and development of a variety of malignant tumors and is anticipated to be an ideal marker for early tumor diagnosis and an effective therapeutic target. Here, we review the research progress into the role of MALAT1 in the diagnosis and treatment of HNSCC and its regulatory mechanism.

Introduction

Head and neck squamous cell carcinoma (HNSCC) is the most common malignant tumors of the head and neck, and its incidence is 6th among all cancers with 890,000 new cases and 450,000 deaths in 2018. The incidence of HNSCC continues to rise and is anticipated to increase by 30% (ie, 1.08 million new cases annually) by 2030.1 HNSCCs mainly including laryngeal cancer, hypopharyngeal cancer, oral cancer, and oropharyngeal cancer.2–4 Most patients have already developed local progression at the time of diagnosis. The tumors are characterized by high mortality and tolerance to radiotherapy and chemotherapy, and the 5-year overall survival rate of patients with HNSCC is only 50%.5,6 Although there are many theories about the etiology of HNSCC, the mechanism of its occurrence and development remains unclear.7 Surgical treatment or comprehensive treatment based on radiotherapy and chemotherapy are the main treatments, but the curative effect is not satisfactory.8 Therefore, exploring HNSCC-related biomarkers as reliable early diagnosis markers, prognostic indicators, and treatment targets, and investigating possible mechanisms of action are of great significance for the early HNSCC detection. Such information will guide individualized treatment and improve patient survival.

Long noncoding RNA (lncRNA) plays an important regulatory role in the biology of head and neck tumors.9 lncRNAs can act tumor-promoting genes to promote the growth, invasion, and migration of cancer cells, or they can serve as tumor suppressor genes to inhibit head and neck tumor occurrence and development.10,11 Various lncRNAs have different mechanisms and regulatory targets in the occurrence, development, and metastasis of head and neck tumors. lncRNAs can regulate gene transcription by binding to enzymes or protein complexes, can participate in chromosome remodeling and epigenetic regulation, and can also inhibit microRNA (miRNA) or alter mRNA modifications to regulate mRNA transcription and translation.12–14 Unique cross-regulation between lncRNA and miRNA was recently described, and emerging evidence shows that such crosstalk has a marked effect on human cancer metastasis, partially through epithelial–mesenchymal transition (EMT) regulation.15 The lncRNAs already found to regulate head and neck tumors can be divided into three major mechanistic categories: cancer-promoting, cancer-suppressing, and dual mechanism of action. Li et al16 discussed the role and function of lncRNAs in laryngeal squamous cell carcinoma (LSCC) proliferation, invasion, and apoptosis and analyzed the relationship between lncRNAs and lncRNA-regulated signaling pathways in LSCC pathogenesis. It provides reference ideas for molecular mechanism research into LSCC that targets lncRNA and its signaling pathways and will contribute to the development of clinical prevention measures therapeutic drugs, and individualized treatment, thereby improving patient quality of life.

Akbari Dilmaghani et al17 reviewed the vital regulatory roles of lncRNAs and introduced their roles in progression of head and neck cancer subtypes.

Meng et al18 described molecular mechanisms of up- or down-regulation of lncRNAs and expounded their functions in HNSCC pathology and clinical practices. They also highlighted their potential clinical applications as biomarkers for HNSCC diagnosis, prognosis, and treatment.

Several studies have shown that treatments based on non-coding RNAs (ncRNAs)—including lncRNAs—may hold promise as alternative methods for treating oral premalignant disorders and oral squamous cell carcinoma (OSCC). Their use as therapeutic agents appear promising.19

Metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) is an important member of the lncRNA family located on chromosome 11q13.1. MALAT1 was first discovered in non-small cell lung carcinoma (NSCLC) and is involved in the regulation of cell cycle and migration.20,21 MALAT1 is unregulated in head and neck malignant tumors; lung, liver, colon, breast, and gastric cancers; and other malignant tumors. MALAT1 expression is closely related to tumor size, stage, and poor prognosis and can be used to evaluate clinicopathological characteristics and prognosis in tumor patients.22–24 Here, we review the role of MALAT1 in the diagnosis and treatment of HNSCC and discuss its possible mechanism.

MALAT1 Structure and Function

MALAT1 is located at 11q13.1 and has 8700 nucleotides. It is an lncRNA that is expressed in the nucleus in high abundance. MALAT1 is highly conserved in mammals, is widely expressed in normal human tissues and cells, and has obvious patterns of tissue specific and temporal expression.25,26 MALAT1 was originally identified in human NSCLC and is abundant in lung cancer tissues. MALAT1 expression is positively correlated with lung cancer metastasis and negatively correlated with prognosis; it is an important prognostic parameter for patients with stage I lung adenocarcinoma or squamous cell carcinoma.27 MALAT1 plays a critical role in transcription and cell cycle regulation, epigenetics, inflammation, and tumor metastasis.28,29 MALAT1 expression is significantly higher in a variety of tumor tissues compared to normal tissues and is associated with tumor metastasis. MALAT1 has a regulatory effect on the occurrence and development of several tumors, including laryngeal, hypopharyngeal, thyroid, esophageal, lung, liver, and ovarian cancers.30–36

The Relationship Between MALAT1 and HNSCC

MALAT1 can regulate the occurrence and development of a variety of malignant tumors. It also plays roles in transcription, cell cycle regulation, epigenetics, and tumor metastasis. In recent years, there has been an increasing amount of research into the growth promotion effect of MALAT1 on HNSCC, highlighting the importance of MALAT1 for tumor diagnosis, prognosis evaluation, and targeted therapy. MALAT1 may function as an oncogene to regulate HNSCC growth, apoptosis, invasion, and metastasis. A number of studies32,37,38 confirmed that MALAT1 expression was significantly up-regulated in HNSCC tissues including laryngeal, nasopharyngeal, and oral samples. MALAT1 is expected to be a marker for early tumor diagnosis and prognosis. Low MALAT1 expression can inhibit the proliferation and metastasis of a variety of HNSCC cells, play a role in suppressing cancer, and improve sensitivity to chemotherapeutics. Therefore, MALAT1 is expected to serve as a new target for tumor treatment.

The Role of MALAT1 in HNSCC Diagnosis and Progression/Prognosis

In-depth study of the correlation between MALAT1 and HNSCC as a diagnostic tool has revealed the characteristics of high accuracy, efficiency, and minimal invasiveness. Although MALAT1 expression has potential value in HNSCC diagnosis, this application is still in the research stage. However, preliminary studies suggest that MALAT1 can be used as an ideal marker for early HNSCC diagnosis.

Yao et al22 showed that MALAT1, HOXA11-AS, and LINC00964 lncRNAs are expected to become potential circulating biomarkers for early HNSCC diagnosis. Among these, MALAT1 is significantly dysregulated in laryngeal squamous cell carcinoma (LSCC) specimens, indicating that it is involved in LSCC occurrence and development,32 but further studies are required to evaluate the clinical role of MALAT1 as a potential diagnostic and prognostic biomarker of LSCC. He et al37 showed that circulating MALAT1, AFAP1-AS1, and AL359062 may be useful as new serum biomarkers for the diagnosis and evaluation of nasopharyngeal carcinoma.

MALAT1 is upregulated in tongue squamous cell carcinoma (TSCC) tissues and is associated with differentiation and clinical stage, suggesting that it can be used as an independent biomarker to assess TSCC prognosis.39 Understanding the critical role of MALAT1 in TSCC may lead to the development of a novel diagnostic marker for this type of cancer.39 Elevated MALAT1 expression in OSCC may play a role in tumorigenesis and metastasis, but further studies are needed to determine which mechanisms affect the growth and migration of MALAT1 in human TSCC SCC4 cells and verify that MALAT1 expression is increased in patients with OSCC.38

The above studies demonstrated that MALAT1 expression levels in patients with HNSCC are correlated with tumor occurrence, development, and prognosis. For most types of HNSCC, higher MALAT1 expression is associated with worse prognosis. However, individual research results indicate that MALAT1 can have biological effects contrary to those mentioned above. Studies by Vishwakarma and others showed that MALAT1 expression is significantly down-regulated in OSCC tissues compared with neighboring normal tissues.40

Cisplatin (DDP) is the first-line chemotherapy agent for OSCC. DDP resistance leads to lower drug efficacy and survival benefits. DDP-resistant cells showed higher MALAT1 expression compared to DDP-naïve cells. MALAT1 overexpression in DDP-naïve cells enhanced drug resistance and suppressed apoptosis in OSCC cells. MALAT1 knockdown in DDP-resistant cells induced apoptotic cell death and restored the sensitivity to DDP. Further analyses suggested that MALAT1 might promote DDP resistance via regulating P-glycoprotein expression, the EMT process, and activation of phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/m-TOR) signaling pathway. MALAT1 might be a potential therapeutic target for the treatment of DDP-resistant OSCC.41

The LSCC TU686 cell line excels in resisting the growth-curbing effects of 5-fluorouracil, paclitaxel, and vincristine compared with the TU177, AMC-HN-8, and LSC-1 cell lines (all P<0.05). Moreover, there seems to be a great potential for overexpressed MALAT1 to enhance the chemoresistance of TU686 and LSC-1 cell lines (P<0.05). Silencing MALAT1 tends to undermine the proliferative and metastatic abilities of TU686 and LSC-1 cell lines (P<0.05). lncRNA MALAT1 is involved in triggering tolerance of LSCC against chemotherapeutics by enhancing and dampening tumor cell metastasis and apoptosis, respectively.42 MALAT1 is overexpressed in HNSCC cell lines compared to a non-tumorigenic cell line. The number of colonies that form after radiation is significantly reduced in MALAT1 knockdown cells, and the half maximal inhibitory concentration of DDP in MALAT1 knockdown cells is also lower than in control cells. MALAT1 knockdown results in cell cycle arrest at G2/M phase, DNA damage and apoptotic cell death. MALAT1 knockdown enhances HNSCC cell sensitivity to radiation and DDP, partly through inducing G2/M cell cycle arrest resulting in DNA damage and apoptosis.42

Jiang et al evaluated the involvement of lncRNA MALAT1 in modifying LSCC cell line chemosensitivity. lncRNA MALAT1 renders LSCC less sensitive to chemotherapeutics by boosting tumor cell metastasis and depressing apoptosis of tumor cells.43

The Role of MALAT1 in HNSCC Treatment

Studies have shown that MALAT1 and related genes and proteins are targets of therapies that can inhibit HNSCC cell proliferation and metastasis and provide new ideas for tumor treatment.

MALAT1 expression is upregulated in HNSCC cell lines compared to non-tumorigenic cell lines.42 Authors first examined the expression of MALAT1 in two HNSCC cell lines (Ca9-22 and FaDu) as compared to non-tumorigenic human skin keratinocyte cell line (HaCaT) using reverse transcription quantitative polymerase-chain reaction (qRT-PCR). They found that MALAT1 expression is significantly higher in Ca9-22 (P<0.05) and FaDu (P<0.0001) cells compared to HaCaT cells. The number of MALAT1 knockdown cell colonies formed after radiation is also significantly reduced. MALAT1 gene knockdown can enhance the sensitivity of HNSCC cells to radiotherapy and DDP. Interfering with MALAT1 expression in head and neck tumors can inhibit tumor growth and metastasis and increase tumor cell apoptosis and sensitivity to radiotherapy.44

Inactivation of MALAT1 can inhibit gene expression regulators that modify the characteristics of laryngeal cancer and hypopharyngeal carcinoma, and blocking MALAT1 expression can inhibit the progression of both types of cancer.33 lncRNA MALAT1 can trigger LSCC resistance to chemotherapy drugs by promoting tumor cell metastasis and inhibiting apoptosis.43 lncRNA MALAT1 serves as a miR-429 sponge, regulating ZEB1 expression. MALAT1 inhibition leads to increased miR-429 and suppression of hypopharyngeal squamous cell carcinoma (HSCC) progression via ZEB1 reduction, providing a potential therapeutic target for HSCC.45 MALAT1 expression levels were determined using RT-qPCR, and cell counting kit-8, transwell, and flow cytometry assays were performed to investigate the biological functions of HSCC cells. The results indicated MALAT1 upregulation in HSCC. MALAT1 knockdown suppresses the malignant behavior of HSCC by targeting miR-194, and MALAT1 is expected to become a new therapeutic target for HSCC.46

In nasopharyngeal carcinoma (NPC), MALAT1 regulates cancer stem cell (CSC) activity and radioresistance by modulating the miR-1/Slug axis, indicating that MALAT1 may also be a therapeutic target for patients with NPC.47

MALAT1 is one of the most widely studied lncRNAs related to various human cancers. Yu et al48 discussed the role of MALAT1 in OSCC and the possible regulatory mechanisms involved. They showed that MALAT1 and miR-143-3p expression were negatively correlated and that MALAT1 expression was positively correlated with that of MAGEA9. An inverse correlation between MAGEA9 and miR-143-3p expression was also observed. These results show that MALAT1 is a promising new therapeutic target for OSCC treatment. Others have shown that MALAT1 is upregulated in OSCC cell lines. Inhibition of MALAT1 can prevent OSCC proliferation, whereas overexpressing MALAT1 promotes OSCC progression. The function of MALAT1 in OSCC development was further investigated with in vivo assays. Studies in established nude mice models revealed that downregulated MALAT1 greatly inhibited OSCC tumor growth; conversely, upregulated MALAT1 promoted OSCC development via the miR-125b/STAT3 axis. MALAT1 can regulate STAT3 expression in OSCC by absorbing miR-125b.49 MALAT1, SRSF1, and CCR7 have exceptionally high expression levels in samples from patients with OSCC with lymphatic metastasis compared to samples without lymph node metastasis as well as para-carcinoma tissues, exclusive of miRNA-320d levels. Interestingly, the target RNAs (MALAT-1, miRNA-320s) and SRSF1 protein can combine with each other, based on the statistically significant difference compared with negative control group (P<0.05). CCR7 expression was higher than the negative control group after silencing miRNA-320d. MALAT-1 interacts with miRNA-320d through SRSF1 and is expected to be a useful target for OSCC prevention and treatment.50 Xiao et al51 explored the biological functions and potential mechanism of MALAT1 in OSCC. Functional studies revealed that MALAT1 overexpression promotes OSCC cell proliferation and invasion. Further experiments revealed that miR-101 is a MALAT1 target, and an miR-101 inhibitor abolishes the effect of MALAT1 on OSCC cell proliferation and invasion. Enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) acted as a downstream effector of MALAT1 in the OSCC cells.

MALAT-1 expression is enhanced in TSCCs, especially in those cases with lymph node metastasis. MALAT-1 knockdown in TSCC cells impairs migration and proliferation in vitro and leads to fewer metastases in vivo. DNA microarray analysis revealed up-regulated expression of several small proline-rich proteins (SPRRs) in TSCC cells following MALAT-1 knockdown. Moreover, SPRR2A over-expression impairs distant TSCC cell metastasis in vivo.52 MALAT1 and p21 (RAC1)-activated kinase 1 (PAK1) are upregulated and miR-140-5p expression is downregulated in TSCC tissues and cells. MALAT1 knockdown induces miR-140-5p expression by direct interaction. Moreover, MALAT1 knockdown inhibits TSCC cell proliferation, migration, and invasion by upregulating miR-140-5p expression.53 MALAT1 knockdown suppresses the growth and invasion of human TSCC cells and inhibits metastasis in vitro and in vivo. In addition, miR-124-dependent jagged1 (JAG1) regulation is required for MALAT1-induced TSCC cell growth. MALAT1 may have a carcinogenic effect by increasing TSCC proliferation and metastasis, and is therefore a potential therapeutic target.54

Regulatory Factors and the Mechanism of MALAT1 in HNSCC

Clarification of biological functions is the basis for identifying ideal tumor markers. Therefore, understanding how MALAT1 affects the growth of HNSCC cells at the mechanistic level will allow us to determine whether MALAT1 can be a useful diagnostic marker and therapeutic target for HNSCC. The occurrence and development of HNSCC is a complex process involving multiple factors, several stages, and the accumulation of mutations in various genes. The mechanisms underlying immortal proliferation of tumor cells are very complicated and include inactivation of tumor suppressor genes, overexpression of oncogenes, loss of cell cycle control, cell cycle failure, and tolerance to apoptosis. A number of studies have shown that a variety of regulatory factors regulate the expression of MALAT1 in HNSCC, including transforming growth factor (TGF)-β/miRNA-124/ERK/MAPK, SRSF1, MEK, and BI-847325. MALAT1 can promote oncogene expression and cell cycle progress and inhibit tumor cell apoptosis to promote tumor growth. HNSCC cells rapidly proliferate following proto-oncogene activation. MALAT1 can target multiple oncogenes or proteins to promote their expression and exert growth-promoting effects, including miR-429/ZEB1, miR-124/Capn4, PI3K/AKT/m-TOR, and Wnt/β-catenin.

Regulatory Factors of MALAT1 in HNSCC

Du et al55 showed that constant TGF-β stimulation represses miR-124 expression, whereas miR-124 overexpression antagonizes TGF-β-promoted NPC cell growth and migration. miR-124 overexpression decreases p-SMAD2/3, SMAD4, and p-ERK levels, indicating that ectopic miR-124 overexpression inhibits SMAD and non-SMAD pathways. Pro-oncogenic lncRNA MALAT1 is targeted by miR-124 that regulates ERK/MAPK by targeting MALAT1, independently of the SMAD signaling pathway. TGF-β can regulate the ERK/MAPK pathway without depending on SMAD by inhibiting miRNA-124. This in turn increases MALAT1 expression in NPC.

In OSCC, SRSF1 can mediate the interaction between MALAT1 and miRNA-320d. Silencing miRNA-320d significantly increases CCR7 expression. Together, these data reveal the molecular-level effect of MALAT1 on the CCR7 chemokine receptor, indicating that MALAT1 may further affect lymph node metastasis of OSCC.50 The MALAT1 regulatory factors in different types of HNSCC are shown in Table 1.

Table 1

MALAT1 Regulatory Factors in HNSCC

Tumor Type	Target Molecule	Reference
NPC	TGF-β/miRNA-124/ERK/MAPK	[55]
OSCC	SRSF1	[50]

Abbreviations: NPC, nasopharyngeal carcinoma; OSCC, oral squamous cell carcinoma.

The Mechanism of MALAT1 in HNSCC

Kangboonruang et al42 demonstrated that knocking down MALAT1 gene expression can induce DNA damage and apoptosis by inducing G2/M cell cycle arrest and enhancing the sensitivity of HNSCC cells to radiotherapy and chemotherapy.

MALAT1 and ZEB1 expression in HSCC are upregulated, while miR-429 expression is downregulated. Conversely, reduced MALAT1 and ZEB1 and upregulated miR-429 inactivate the PI3K/Akt/mTOR signaling pathway; suppress in vitro viability, colony formation, migration, and invasion; decrease cell growth in vivo; and promote the apoptosis of FaDu cells. Downregulating miR-429 reverses the role of MALAT1 inhibition in FaDu cell growth. lncRNA MALAT1 serves as a sponge of miR-429, thus regulating ZEB1 expression. MALAT1 inhibition can inhibit HSCC progression by increasing miR-429 and decreasing ZEB1.45 MALAT1 knockdown suppresses HSCC cell proliferation, migration, and invasion and promotes apoptosis compared with the control group. Additionally, miR-194 was identified as a target of MALAT1 expressed at low levels in HSCC tissues compared with adjacent non-tumor tissues. An miR-194 agomir inhibits malignant cell behaviors including proliferation, migration and invasion, whereas an miR-194 antagomir promotes malignant behaviors compared with the corresponding control groups. MALAT1 knockout can also inhibit HSCC progression by targeting miR-194.46 Inhibition of miR-194 partially reverses MALAT1 knockdown-induced inhibitory effects in HSCC cells.

Shi et al56 showed that MALAT1 absorbs miR-124 through a sponge effect; inhibits Capn4; and promotes the proliferation, invasion, and EMT of NPC cells. This revealed a new MALAT1/miR-124/Capn4 regulatory axis involved in NPC pathogenesis. MALAT1 can also be used as a competitive endogenous RNA (ceRNA) in NPC to increase Slug mRNA and protein levels. The combination of MALAT1 and miRNA-1 reduces miRNA-1 and Slug mRNA levels, while MALAT1 can eliminate miRNA-1 induced Slug gene silencing.47

With regard to OSCC, two kinds of human DDP-resistant cell lines (CAL-27R and SCC-9R) were developed from DDP-naïve cell lines (CAL-27 and SCC-9, respectively) as in vitro cell models. Cell transfection was performed to overexpress or knockdown MALAT1, and mouse xenograft models were established. The following measurements were performed: cell proliferation, colony formation, wound healing, transwell, and terminal deoxynucleotidyl transferase dUTP nick end labeling assays, as well as Western blotting and immunofluorescence staining. MALAT1 can promote the EMT and enhance resistance to DDP through the PI3K/AKT/m-TOR signaling pathway.41 Yu et al48 discussed the role of MALAT1 in OSCC and possible regulatory mechanisms and showed that MALAT1 acts as a ceRNA to promote OSCC cell proliferation and migration through the miR-143-3p/MAGEA9 axis. A bioinformatics search revealed that miR-125b is a direct target of MALAT1, suggesting a negative correlation between MALAT1 and miR-125b. STAT3 was also predicted as a binding target of miR-125b in OSCC. Overexpression of MALAT1 was able to suppress the tumor inhibitory effect of miR-125b mimics by upregulating STAT3. MALAT1 can regulate STAT3 expression by absorbing miR-125b to promote OSCC occurrence and development.49 Xiao et al51 performed qPCR analyses and found obviously increased MALAT1 expression and decreased miR‑101 in OSCC tissues and cell lines. This suggests that upregulation of MALAT1 can promote OSCC proliferation and invasion by targeting the miR-101/EZH2 axis.

lncRNA MALAT1 is upregulated in TSCC tissues and correlates with cervical lymph node metastasis in patients with TSCC. Moreover, MALAT1 induces cell migration, invasion, and EMT and inhibits apoptosis by modulating Wnt/β-catenin signaling. The effect of exogenous MALAT1 is also inhibited by the Wnt/β-catenin signaling pathway. In TSCC, MALAT1 promotes the EMT and inhibits apoptosis by regulating the Wnt/β-catenin signaling pathway.57 Yuan et al58 studied the function of MALAT1 in TSCC and found that MALAT1 expression was remarkably higher in tumor tissues compared to adjacent healthy tissues. Serum MALAT1 was significantly higher in patients with TSCC versus healthy controls. MALAT1 knockdown markedly inhibits TSCC cell proliferation, migration, and invasion. MALAT1 knockdown also reduces Akt phosphorylation levels and matrix metalloproteinase-9 expression. High MALAT1 expression is related to TSCC growth and metastasis, but MALAT1 knockout can upregulate some SPRR proteins, thereby affecting the distant metastasis of TSCC cells.52 The expression of MALAT1 and p21 (RAC1)-activated kinase 1 (PAK1) are upregulated and miR-140-5p expression is downregulated in TSCC tissues and cells. MALAT1 can promote TSCC progression through the miR-140-5p-PAK1 regulatory axis.53 Zhang et al54 showed that MALAT1 interacts with miR-124 to regulate TSCC growth by targeting JAG1, and that MALAT1 exerts carcinogenic effects by increasing TSCC proliferation and metastasis. MALAT1 target molecules vary in different types of HNSCC. The target molecules and effects of MALAT1 in common types of HNSCC are shown in Table 2. The mechanisms of MALAT1 in HNSCC are shown in Figure 1.

Table 2

MALAT1 Target Molecules and Consequences of Their Targeting in HNSCC

Tumor Type	Target Molecule	Effect	Reference
HNSCC	G2/M	Sensitivity	[42]
HSCC	miR-429/ZEB1	Differentiation, proliferation, invasion, metastasis	[45]
	miR-194	Differentiation, proliferation, invasion, metastasis	[46]
NPC	miR-124/Capn4	Proliferation, invasion, EMT	[56]
	miRNA-1/Slug mRNA	Differentiation, proliferation, invasion, metastasis	[47]
OSCC	PI3K/AKT/m-TOR	EMT, sensitivity	[41]
	miR-143-3p/MAGEA9	Proliferation, invasion, metastasis	[48]
	miR-125b/STAT3	Differentiation, proliferation, invasion, metastasis	[49]
	CCR7	Metastasis	[50]
	miR-101/EZH2	Proliferation, invasion	[51]
TSCC	Wnt/β-catenin	EMT, apoptosis	[57]
	PI3K/Akt, MMP-9	Proliferation, invasion, metastasis	[58]
	SPRR	Metastasis	[52]
	miR-140-5p-PAK1	Proliferation, invasion, metastasis	[53]
	miR-124/JAG1	Proliferation, metastasis	[54]

Abbreviations: HNSCC, head and neck squamous cell carcinoma; HSCC, hypopharyngeal squamous cell carcinoma; NPC, nasopharyngeal carcinoma; OSCC, oral squamous cell carcinoma; TSCC, tongue squamous cell carcinoma.

Figure 1

The mechanisms of MALAT1 in HNSCC.

Conclusion

Abnormal MALAT1 expression plays an important role in HNSCC occurrence and development. In most types of HNSCC, higher MALAT1 expression is associated with poorer prognosis. With increased MALAT1-focused research, additional roles and mechanisms will gradually be elucidated, and these will have implications for HNSCC diagnosis, treatment, and prognosis. With continuous advancement of biological science and technology including more accurate sequencing methods, future studies exploring the precise and detailed mechanism of MALAT1 action and function will aid in the early diagnosis of HNSCC and identify new treatment targets and biomarkers. Together, these advances are likely to lead to the establishment of personalized precision medical treatment and improved prognostic evaluation for patients with HNSCC.