Literature DB >> 34264467

A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins.

Mei G Lei1, Ravi Kr Gupta1,2, Chia Y Lee3.   

Abstract

Secreted bacterial proteins are difficult to identify directly from an infection site due to a limited amount of bacteria and presence of a large quantity of host proteins. Here we describe a rat model of orthopedic implant that allows us to harvest bacterial biofilm materials sufficient for identification of bacterial proteins in the biofilm matrix by liquid chromatography-tandem MS (GeLC-MS/MS) analysis.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bacteria; Biofilm matrix proteins; Orthopedic implant-associated infection; Proteomics; Staphylococcus aureus

Year:  2021        PMID： 34264467     DOI： 10.1007/978-1-0716-1550-8_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Mouse model of chronic post-arthroplasty infection: noninvasive in vivo bioluminescence imaging to monitor bacterial burden for long-term study.

Authors:  Jonathan R Pribaz; Nicholas M Bernthal; Fabrizio Billi; John S Cho; Romela Irene Ramos; Yi Guo; Ambrose L Cheung; Kevin P Francis; Lloyd S Miller
Journal:  J Orthop Res       Date:  2011-08-11       Impact factor: 3.494

Review 2.  The biological role of death and lysis in biofilm development.

Authors:  Kenneth W Bayles
Journal:  Nat Rev Microbiol       Date:  2007-09       Impact factor: 60.633

Review 3.  Staphylococcal biofilms.

Authors:  M Otto
Journal:  Curr Top Microbiol Immunol       Date:  2008       Impact factor: 4.291

Review 4.  Staphylococcus and biofilms.

Authors:  Friedrich Götz
Journal:  Mol Microbiol       Date:  2002-03       Impact factor: 3.501

Review 5.  Staphylococcal biofilm disassembly.

Authors:  Blaise R Boles; Alexander R Horswill
Journal:  Trends Microbiol       Date:  2011-07-23       Impact factor: 17.079

6.  Role of the accessory gene regulator (agr) in pathogenesis of staphylococcal osteomyelitis.

Authors:  A F Gillaspy; S G Hickmon; R A Skinner; J R Thomas; C L Nelson; M S Smeltzer
Journal:  Infect Immun       Date:  1995-09       Impact factor: 3.441

Review 7.  Osteomyelitis.

Authors:  Daniel P Lew; Francis A Waldvogel
Journal:  Lancet       Date:  2004 Jul 24-30       Impact factor: 79.321

Review 8.  ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus.

Authors:  James P O'Gara
Journal:  FEMS Microbiol Lett       Date:  2007-04-10       Impact factor: 2.742

Review 9.  Protein-based biofilm matrices in Staphylococci.

Authors:  Pietro Speziale; Giampiero Pietrocola; Timothy J Foster; Joan A Geoghegan
Journal:  Front Cell Infect Microbiol       Date:  2014-12-10       Impact factor: 5.293

10.  Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.

Authors:  Mei G Lei; Ravi Kr Gupta; Chia Y Lee
Journal:  PLoS One       Date:  2017-11-09       Impact factor: 3.240
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