Literature DB >> 34264464

Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus.

Logan L Bulock1, Jennifer L Endres1, Marat R Sadykov2.   

Abstract

Oxygen consumption is a fundamental characteristic of staphylococcal physiology reflecting the energy and metabolic state of the bacterial cell. During aerobic growth, oxygen consumption rates (OCR) depend on nutrient availability and vary at different growth stages. The measurement of oxygen consumption rates provides a versatile tool to characterize the impact of various mutations, environmental cues, and antibiotics on bacterial growth and fitness. In this chapter, we describe a MitoXpress® Xtra-based oxygen consumption assay for fast and reliable determination of respiration rates in Staphylococcus aureus. This highly reproducible and simple method requires a minimal set of reagents and allows rapid screening of multiple samples through real-time determination of the OCR with an oxygen-sensing probe and fluorescence plate reader.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Energy status; Metabolism; Oxygen consumption rates; Respiration; Staphylococcus aureus

Year:  2021        PMID： 34264464     DOI： 10.1007/978-1-0716-1550-8_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target.

Authors:  Pavel Dibrov; Elena Dibrov; Thane G Maddaford; Melissa Kenneth; Jordan Nelson; Craig Resch; Grant N Pierce
Journal:  Can J Physiol Pharmacol       Date:  2017-04-20       Impact factor: 2.273

2.  Rapid detection and respirometric profiling of aerobic bacteria on panels of selective media.

Authors:  G Jasionek; V Ogurtsov; D Papkovsky
Journal:  J Appl Microbiol       Date:  2012-11-21       Impact factor: 3.772

3.  Novel antibiotics targeting respiratory ATP synthesis in Gram-positive pathogenic bacteria.

Authors:  Wendy Balemans; Luc Vranckx; Nacer Lounis; Ovidiu Pop; Jérôme Guillemont; Karen Vergauwen; Selena Mol; Ron Gilissen; Magali Motte; David Lançois; Miguel De Bolle; Kristien Bonroy; Holger Lill; Koen Andries; Dirk Bald; Anil Koul
Journal:  Antimicrob Agents Chemother       Date:  2012-05-21       Impact factor: 5.191

4.  Poly(3-hydroxybutyrate) fuels the tricarboxylic acid cycle and de novo lipid biosynthesis during Bacillus anthracis sporulation.

Authors:  Marat R Sadykov; Jong-Sam Ahn; Todd J Widhelm; Valerie M Eckrich; Jennifer L Endres; Adam Driks; Gregory E Rutkowski; Kevin L Wingerd; Kenneth W Bayles
Journal:  Mol Microbiol       Date:  2017-03-30       Impact factor: 3.501

5.  Amino Acid Catabolism in Staphylococcus aureus and the Function of Carbon Catabolite Repression.

Authors:  Cortney R Halsey; Shulei Lei; Jacqueline K Wax; Mckenzie K Lehman; Austin S Nuxoll; Laurey Steinke; Marat Sadykov; Robert Powers; Paul D Fey
Journal:  MBio       Date:  2017-02-14       Impact factor: 7.867

6.  A central role for carbon-overflow pathways in the modulation of bacterial cell death.

Authors:  Vinai Chittezham Thomas; Marat R Sadykov; Sujata S Chaudhari; Joselyn Jones; Jennifer L Endres; Todd J Widhelm; Jong-Sam Ahn; Randeep S Jawa; Matthew C Zimmerman; Kenneth W Bayles
Journal:  PLoS Pathog       Date:  2014-06-19       Impact factor: 6.823
  6 in total

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