Jasmina Sretenovic1, Jovana Joksimovic Jovic2, Ivan Srejovic1, Vladimir Zivkovic1, Katarina Mihajlovic3, Milica Labudovic-Borovic4, Svetlana Trifunovic5, Verica Milosevic5, Dejan Lazic6, Sergey Bolevich7, Vladimir Jakovljevic1,7, Zoran Milosavljevic8. 1. Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34 000, Kragujevac, Serbia. 2. Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34 000, Kragujevac, Serbia. jovana_joksimovic@yahoo.com. 3. Faculty of Medical Sciences, Department of Pharmacy, University of Kragujevac, Svetozara Markovica 69, 34 000, Kragujevac, Serbia. 4. Institute of Histology and Embryology "Aleksandar Đ. Kostić" Faculty of Medicine, Supremalab Diagnostics and Research, University of Belgrade, 11000, Belgrade, Serbia. 5. Department of Cytology, Institute for Biological Research "Sinisa Stankovic"- National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000, Belgrade, Serbia. 6. Faculty of Medical Sciences, Department of Surgery, University of Kragujevac, Svetozara Markovica 69, 34 000, Kragujevac, Serbia. 7. Department of Human Pathology, 1st Moscow State Medical University IM Sechenov, Trubetskaya str. 2, Russian Federation, 119992, Moscow, Russia. 8. Faculty of Medical Sciences, Department of Histology and Embryology, University of Kragujevac, Svetozara Markovica 69, 34 000, Kragujevac, Serbia.
Abstract
BACKGROUND: During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS: Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS: Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction.
BACKGROUND: During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS: Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS:Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction.
Authors: Ana Paula Tanno; Vander José das Neves; Kaleizu Teodoro Rosa; Tatiana Sousa Cunha; Fernanda Cristina Linarello Giordano; Caroline Morini Calil; Vinicius Guzzoni; Tiago Fernandes; Edilamar Menezes de Oliveira; Pedro Duarte Novaes; Maria Cláudia Irigoyen; Maria José Costa Sampaio Moura; Fernanda Klein Marcondes Journal: Life Sci Date: 2011-08-17 Impact factor: 5.037
Authors: F L Rocha; E C Carmo; F R Roque; N Y Hashimoto; L V Rossoni; C Frimm; I Anéas; C E Negrão; J E Krieger; E M Oliveira Journal: Am J Physiol Heart Circ Physiol Date: 2007-09-28 Impact factor: 4.733
Authors: Jasmina Sretenovic; Vladimir Zivkovic; Ivan Srejovic; Suzana Pantovic; Jovana Joksimovic Jovic; Maja Nikolic; Tamara Nikolic Turnic; Maja Savic; Maja Jevdjevic; Zoran Milosavljevic; Sergej Bolevich; Vladimir Jakovljevic Journal: Life (Basel) Date: 2022-08-16