Literature DB >> 3426000

The complete amino acid sequence of the Vigna unguiculata (L.) Walp. seed trypsin and chymotrypsin inhibitor.

L Morhy1, M M Ventura.   

Abstract

The amino acid sequence of the "double-headed" trypsin and chymotrypsin inhibitor (BTCI), purified from the cowpea Vigna unguiculata (L.) Walp. cv "Seridó" was determined in our laboratory. Tryptic and chymotryptic peptides were sequenced by the Edman-Gray and Edman-Chang N-terminal manual method as well as the carboxypeptidase C-terminal method. The complete amino acid sequence of the BTCI is: Ser-Gly-His-His-Glx-Asx-Ser-Thr-Asx-Glx-Ala-Ser-Glx-Ser-Ser-Lys-Pro-Cys- Cys-Arg- Glx-Cys-Ala-Cys-Thr-Lys-Ser-Ile-Pro-Pro-Glx-Cys-Arg-Cys-Ser-Asx-Val-Arg- Leu-Asn- Ser-Cys-His-Ser-Ala-Cys-Lys-Ser-Cys-Ala-Cys-Thr-Phe-Ser-Ile-Pro-Ala-Glx- Cys-Phe- Cys-Gly-Asx-Ile-Asx-Asx-Phe-Cys-Tyr-Lys-Pro-Cys-Lys-Ser-Ser-His-Ser-Asx- Asx-Asx-Asx-Trp-Asn. BTCI presents a high degree of homology with the Bowman-Birk proteinase inhibitor family.

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Year:  1987        PMID： 3426000

Source DB:  PubMed          Journal:  An Acad Bras Cienc        ISSN： 0001-3765            Impact factor:   1.753


  6 in total

1.  Crystal structure of the Bowman-Birk Inhibitor from Vigna unguiculata seeds in complex with beta-trypsin at 1.55 A resolution and its structural properties in association with proteinases.

Authors:  João Alexandre R G Barbosa; Luciano P Silva; Rozeni C L Teles; Gisele F Esteves; Ricardo B Azevedo; Manuel M Ventura; Sonia M de Freitas
Journal:  Biophys J       Date:  2006-12-01       Impact factor: 4.033

2.  Purification, characterization, sequence determination, and mass spectrometric analysis of a trypsin inhibitor from seeds of the Brazilian tree Dipteryx alata (Leguminosae).

Authors:  D E Kalume; M V Sousa; L Morhy
Journal:  J Protein Chem       Date:  1995-11

3.  Amino acid sequence of the Phaseolus vulgaris var. "fogo na serra" inhibitor and interactive surface modeling for the enzyme-inhibitor complex.

Authors:  P G de Carvalho; C Bloch; L Morhy; M C da Silva; L V de Mello; G Neshich
Journal:  J Protein Chem       Date:  1996-08

4.  Blood pressure-lowering effects of a Bowman-Birk inhibitor and its derived peptides in normotensive and hypertensive rats.

Authors:  Maria Alzira Garcia de Freitas; Nathalia Oda Amaral; Alice da Cunha Morales Álvares; Sandriele Aires de Oliveira; Azadeh Mehdad; Diego Elias Honda; Amanda Sá Martins Bessa; Marcelo Henrique Soller Ramada; Lara Marques Naves; Carolina Nobre Ribeiro Pontes; Carlos Henrique Castro; Gustavo Rodrigues Pedrino; Sonia Maria de Freitas
Journal:  Sci Rep       Date:  2020-07-15       Impact factor: 4.379

5.  Neutrophil elastase inhibitor purification strategy from cowpea seeds.

Authors:  Graziele Cristina Ferreira; Adriana Feliciano Alves Duran; Flavia Ribeiro Santos da Silva; Livia de Moraes Bomediano; Gabriel Capella Machado; Sergio Daishi Sasaki
Journal:  PLoS One       Date:  2019-10-10       Impact factor: 3.240

6.  Effects of an anticarcinogenic Bowman-Birk protease inhibitor on purified 20S proteasome and MCF-7 breast cancer cells.

Authors:  Larissa da Costa Souza; Ricardo Camargo; Marilene Demasi; Jaime Martins Santana; Cézar Martins de Sá; Sonia Maria de Freitas
Journal:  PLoS One       Date:  2014-01-27       Impact factor: 3.240
  6 in total

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