Literature DB >> 34250200

Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer.

Ana S Almeida1, Miriam R Fein1, Mikala Egeblad1.   

Abstract

Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by multi-color flow cytometry. This protocol can be used to study the following tumor-infiltrating immune cell populations, defined by the expression of cell surface molecules: total leukocytes, tumor-associated macrophages (TAMs), conventional dendritic cells (DCs), CD103-expressing DCs, tumor-associated neutrophils, inflammatory monocytes, natural killer (NK) cells, CD4+ T cells, CD8+ T cells, γδT cells, and regulatory T cells.
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Antibodies; Breast cancer; Flow cytometry; Immune cell subsets; Immunophenotyping; Mouse model; Tumor microenvironment

Year:  2021        PMID： 34250200      PMCID： PMC8251661          DOI： 10.21769/BioProtoc.4012

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  12 in total

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10.  Cancer cell CCR2 orchestrates suppression of the adaptive immune response.

Authors:  Miriam R Fein; Xue-Yan He; Ana S Almeida; Emilis Bružas; Arnaud Pommier; Ran Yan; Anaïs Eberhardt; Douglas T Fearon; Linda Van Aelst; John Erby Wilkinson; Camila O Dos Santos; Mikala Egeblad
Journal:  J Exp Med       Date:  2020-10-05       Impact factor: 14.307
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  1 in total

1.  High-affinity SOAT1 ligands remodeled cholesterol metabolism program to inhibit tumor growth.

Authors:  Zhihua Wang; Miaomiao Wang; Mengxin Zhang; Kaikun Xu; Xinshuai Zhang; Yi Xie; Yiming Zhang; Cheng Chang; Xiaolu Li; Aihua Sun; Fuchu He
Journal:  BMC Med       Date:  2022-08-09       Impact factor: 11.150
  1 in total

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