Xingchen Bian1,2,3,4, Xiaofen Liu2,3,4, Xuefei Zhang2, Xin Li2,3,4, Jing Zhang2,3,4,5, Huajun Zheng6, Sichao Song6, Xiang Li7, Meiqing Feng8. 1. School of Pharmacy & Minhang Hospital, Fudan University, 826 Zhang Heng Rd, 201203, Shanghai, China. 2. Institute of Antibiotics, Huashan Hospital, Fudan University, 200040, Shanghai, China. 3. Key Laboratory of Clinical Pharmacology of Antibiotics, 200040, Shanghai, China. 4. Huashan Hospital, National Health Commission & National Clinical Research Center for Aging and Medicine, Fudan University, 200040, Shanghai, China. 5. Phase I Unit, Huashan Hospital, Fudan University, 200040, Shanghai, China. 6. Chinese National Human Genome Center, 201203, Shanghai, China. 7. School of Pharmacy & Minhang Hospital, Fudan University, 826 Zhang Heng Rd, 201203, Shanghai, China. xiangli_mh@fudan.edu.cn. 8. School of Pharmacy & Minhang Hospital, Fudan University, 826 Zhang Heng Rd, 201203, Shanghai, China. fmq@fudan.edu.cn.
Abstract
BACKGROUND: Acinetobacter baumannii is a common nosocomial pathogen that poses a huge threat to global health. Owing to the severity of A. baumannii infections, it became necessary to investigate the epidemiological characteristics of A. baumannii in Chinese hospitals and find the reasons for the high antibiotic resistance rate and mortality. This study aimed to investigate the epidemiologic and genetic characteristics of A. baumannii isolated from patients with hospital acquired pneumonia (HAP), bloodstream infection (BSI) and urinary tract infection (UTI) in China and uncover potential mechanisms for multi-drug resistance and virulence characteristics of A. baumannii isolates. RESULTS: All isolates were classified into two primary clades in core gene-based phylogenetic relationship. Clonal complex 208 (CC208) mainly consisted of ST195 (32 %) and ST208 (24.6 %). CC208 and non-CC208 isolates had carbapenem resistance rates of 96.2 and 9.1 %, respectively. Core genes were enriched in 'Amino acid transport and metabolism', 'Translation', 'Energy production and conversion', 'Transcription', 'Inorganic ion transport and metabolism' and 'Cell wall/membrane/envelope synthesis'. Most isolates possessed virulence factors related to polysaccharide biosynthesis, capsular polysaccharide synthesis and motility. Eleven isolates belong to ST369 or ST191 (oxford scheme) all had the virulence factor cap8E and it had a higher positive rate in UTI (35.3 %) than in BSI (18.9 %) and HAP (12.9 %). ABGRI1 antibiotic resistance islands were responsible for streptomycin, tetracycline and sulfonate resistance. The blaOXA-23 gene was the most probable cause for carbapenem resistance, although the blaOXA-66 gene with nonsynonymous SNPs (F82L, I129L) was not. CONCLUSIONS: A. baumannii is a genomically variable pathogen that has the potential to cause a range of infectious diseases. There is high proportion of carbapenem resistance in isolates from all three infection sites (HAP, BSI and UTI), which can be attributed to the blaOXA-23 gene. CC208 is the predominant clone in blaOXA-23-carrying A. baumannii that should be monitored. Virulence factors involving bacteria motility and polysaccharide biosynthesis which are widespread in clinical A. baumannii strains deserve our attention.
BACKGROUND:Acinetobacter baumannii is a common nosocomial pathogen that poses a huge threat to global health. Owing to the severity of A. baumannii infections, it became necessary to investigate the epidemiological characteristics of A. baumannii in Chinese hospitals and find the reasons for the high antibiotic resistance rate and mortality. This study aimed to investigate the epidemiologic and genetic characteristics of A. baumannii isolated from patients with hospital acquired pneumonia (HAP), bloodstream infection (BSI) and urinary tract infection (UTI) in China and uncover potential mechanisms for multi-drug resistance and virulence characteristics of A. baumannii isolates. RESULTS: All isolates were classified into two primary clades in core gene-based phylogenetic relationship. Clonal complex 208 (CC208) mainly consisted of ST195 (32 %) and ST208 (24.6 %). CC208 and non-CC208 isolates had carbapenem resistance rates of 96.2 and 9.1 %, respectively. Core genes were enriched in 'Amino acid transport and metabolism', 'Translation', 'Energy production and conversion', 'Transcription', 'Inorganic ion transport and metabolism' and 'Cell wall/membrane/envelope synthesis'. Most isolates possessed virulence factors related to polysaccharide biosynthesis, capsular polysaccharide synthesis and motility. Eleven isolates belong to ST369 or ST191 (oxford scheme) all had the virulence factor cap8E and it had a higher positive rate in UTI (35.3 %) than in BSI (18.9 %) and HAP (12.9 %). ABGRI1 antibiotic resistance islands were responsible for streptomycin, tetracycline and sulfonate resistance. The blaOXA-23 gene was the most probable cause for carbapenem resistance, although the blaOXA-66 gene with nonsynonymous SNPs (F82L, I129L) was not. CONCLUSIONS:A. baumannii is a genomically variable pathogen that has the potential to cause a range of infectious diseases. There is high proportion of carbapenem resistance in isolates from all three infection sites (HAP, BSI and UTI), which can be attributed to the blaOXA-23 gene. CC208 is the predominant clone in blaOXA-23-carrying A. baumannii that should be monitored. Virulence factors involving bacteria motility and polysaccharide biosynthesis which are widespread in clinical A. baumannii strains deserve our attention.
Authors: Luis A Arroyo; Carmen M Herrera; Lucia Fernandez; Jessica V Hankins; M Stephen Trent; Robert E W Hancock Journal: Antimicrob Agents Chemother Date: 2011-06-06 Impact factor: 5.191
Authors: Mark D Adams; Gabrielle C Nickel; Saralee Bajaksouzian; Heather Lavender; A Rekha Murthy; Michael R Jacobs; Robert A Bonomo Journal: Antimicrob Agents Chemother Date: 2009-06-15 Impact factor: 5.191
Authors: Marcelo Silva Folhas Damas; Roumayne Lopes Ferreira; Emeline Boni Campanini; Gabriela Guerrera Soares; Leslie Camelo Campos; Pedro Mendes Laprega; Andrea Soares da Costa; Caio César de Melo Freire; André Pitondo-Silva; Louise Teixeira Cerdeira; Anderson Ferreira da Cunha; Maria-Cristina da Silva Pranchevicius Journal: Front Med (Lausanne) Date: 2022-07-28