Olouwafemi Mistourath Mama1, Carmen Aspiroz2, Carmen Lozano1, Laura Ruiz-Ripa1, José Manuel Azcona3, Cristina Seral4, Emilia Cercenado5, Lorena López-Cerero6, Pilar Palacian7, Alba Belles-Belles8, Pilar Berdonces9, María Siller10, Amaia Aguirre-Quiñonero11, Myriam Zarazaga1, Carmen Torres1. 1. Área Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 53, 26006, Logroño, Spain. 2. Servicio Microbiología, Hospital Royo Villanova, Zaragoza, Spain. 3. Servicio Microbiología, Hospital San Pedro, Logroño, Spain. 4. Servicio Microbiología, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain. 5. Servicio Microbiología, Hospital General Universitario Gregorio Marañón, CIBERES, Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, Madrid, Spain. 6. Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Departamento de Microbiología, Universidad de Sevilla, Instituto de Biomedicina de Sevilla, Sevilla, Spain. 7. Servicio Microbiología, Hospital Universitario Miguel Servet/IIS Aragón, Zaragoza, Spain. 8. Servicio Microbiología, Hospital Universitario Arnau de Vilanova, Lérida, Spain. 9. Servicio Microbiología, Hospital Galdakao, Galdakao, Spain. 10. Servicio Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain. 11. Servicio Microbiología, Hospital Universitario de Álava, Vitoria, Spain.
Abstract
OBJECTIVES: To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. METHODS: A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018-19 (6-12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. RESULTS: The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton-Valentine leucocidin ('PVL') positive. CONCLUSIONS: A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.
OBJECTIVES: To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. METHODS: A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018-19 (6-12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. RESULTS: The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton-Valentine leucocidin ('PVL') positive. CONCLUSIONS: A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.
Authors: Spencer A Bruce; Joshua T Smith; Jennifer L Mydosh; John Ball; David B Needle; Robert Gibson; Cheryl P Andam Journal: Sci Rep Date: 2022-03-15 Impact factor: 4.379
Authors: Geoffrey W Coombs; Nicholas W T Yee; Denise Daley; Catherine M Bennett; James O Robinson; Marc Stegger; Princy Shoby; Shakeel Mowlaboccus Journal: Microorganisms Date: 2022-08-15
Authors: Sara Ceballos; Carmen Lozano; Carmen Aspiroz; Laura Ruiz-Ripa; Paula Eguizábal; Allelen Campaña-Burguet; Emilia Cercenado; Ana Isabel López-Calleja; Javier Castillo; Jose Manuel Azcona-Gutiérrez; Luis Torres; Jorge Calvo; Carmen Martin; María Navarro; Myriam Zarazaga; Carmen Torres Journal: Pathogens Date: 2022-03-01