Literature DB >> 34236361

A facile chemoenzymatic synthesis of SARS-CoV-2 glycopeptides for probing glycosylation functions.

Guanghui Zong1, Chao Li1, Sunaina Kiran Prabhu1, Roushu Zhang1, Xiao Zhang1, Lai-Xi Wang1.   

Abstract

Glycosylation plays important roles in SARS-CoV-2 infection. We describe here a facile chemoenzymatic synthesis of core-fucosylated N-glycopeptides derived from the SARS-CoV-2 Spike protein and their binding with glycan-dependent neutralizing antibody S309 and human lectin CLEC4G. The synthetic glycopeptides provide tools for further functional characterization of viral glycosylation.

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Year:  2021        PMID： 34236361      PMCID： PMC8294178          DOI： 10.1039/d1cc02790e

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.065


  20 in total

1.  Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins.

Authors:  John P Giddens; Joseph V Lomino; Mohammed N Amin; Lai-Xi Wang
Journal:  J Biol Chem       Date:  2016-03-10       Impact factor: 5.157

Review 2.  Chemoenzymatic Methods for the Synthesis of Glycoproteins.

Authors:  Chao Li; Lai-Xi Wang
Journal:  Chem Rev       Date:  2018-08-24       Impact factor: 60.622

3.  Efficient glycosynthase mutant derived from Mucor hiemalis endo-beta-N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan.

Authors:  Midori Umekawa; Cishan Li; Takayuki Higashiyama; Wei Huang; Hisashi Ashida; Kenji Yamamoto; Lai-Xi Wang
Journal:  J Biol Chem       Date:  2009-10-30       Impact factor: 5.157

4.  Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins.

Authors:  Chao Li; Shilei Zhu; Christopher Ma; Lai-Xi Wang
Journal:  J Am Chem Soc       Date:  2017-10-16       Impact factor: 15.419

5.  Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.

Authors:  Farong Ye; Jie Zhao; Peng Xu; Xinliang Liu; Jing Yu; Wei Shangguan; Jiazhi Liu; Xiaosheng Luo; Cheng Li; Tianlei Ying; Jing Wang; Biao Yu; Ping Wang
Journal:  Angew Chem Int Ed Engl       Date:  2021-03-11       Impact factor: 15.336

6.  LSECtin interacts with filovirus glycoproteins and the spike protein of SARS coronavirus.

Authors:  Thomas Gramberg; Heike Hofmann; Peggy Möller; Patricia F Lalor; Andrea Marzi; Martina Geier; Mandy Krumbiegel; Thomas Winkler; Frank Kirchhoff; David H Adams; Stephan Becker; Jan Münch; Stefan Pöhlmann
Journal:  Virology       Date:  2005-09-30       Impact factor: 3.616

7.  Site-specific glycan analysis of the SARS-CoV-2 spike.

Authors:  Yasunori Watanabe; Joel D Allen; Daniel Wrapp; Jason S McLellan; Max Crispin
Journal:  Science       Date:  2020-05-04       Impact factor: 47.728

8.  Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.

Authors:  Dapeng Zhou; Xiaoxu Tian; Ruibing Qi; Chao Peng; Wen Zhang
Journal:  Glycobiology       Date:  2021-01-09       Impact factor: 4.313

9.  The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity.

Authors:  Qianqian Li; Jiajing Wu; Jianhui Nie; Li Zhang; Huan Hao; Shuo Liu; Chenyan Zhao; Qi Zhang; Huan Liu; Lingling Nie; Haiyang Qin; Meng Wang; Qiong Lu; Xiaoyu Li; Qiyu Sun; Junkai Liu; Linqi Zhang; Xuguang Li; Weijin Huang; Youchun Wang
Journal:  Cell       Date:  2020-07-17       Impact factor: 41.582

10.  Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.

Authors:  Lorenzo Casalino; Zied Gaieb; Jory A Goldsmith; Christy K Hjorth; Abigail C Dommer; Aoife M Harbison; Carl A Fogarty; Emilia P Barros; Bryn C Taylor; Jason S McLellan; Elisa Fadda; Rommie E Amaro
Journal:  ACS Cent Sci       Date:  2020-09-23       Impact factor: 14.553
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  2 in total

1.  Site-selective sulfation of N-glycans by human GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis of sulfated antibody glycoforms.

Authors:  Kun Huang; Chao Li; Guanghui Zong; Sunaina Kiran Prabhu; Digantkumar G Chapla; Kelley W Moremen; Lai-Xi Wang
Journal:  Bioorg Chem       Date:  2022-08-01       Impact factor: 5.307

2.  Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate.

Authors:  Guanghui Zong; Christian Toonstra; Qiang Yang; Roushu Zhang; Lai-Xi Wang
Journal:  Int J Mol Sci       Date:  2021-11-21       Impact factor: 5.923
  2 in total

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