| Literature DB >> 34235668 |
M Carla Martini1, Huaming Sun1,2, Scarlet S Shell3,4.
Abstract
Next-generation sequencing technologies facilitate the analysis of multiple important properties of transcriptomes in addition to gene expression levels. Here, we describe a method for mapping RNA 5' ends in Mycobacterium tuberculosis and Mycobacterium smegmatis, which allows the determination of transcription start sites (TSSs), comparative analysis of promoter usage under different conditions, and mapping of endoribonucleolytic cleavage sites. We describe in detail the procedures for constructing RNA sequencing libraries appropriate for RNA 5' end mapping using an Illumina sequencing platform, as well as bioinformatic procedures for data analysis.Entities:
Keywords: Endoribonuclease; Mycobacterium smegmatis; Mycobacterium tuberculosis; RNA 5′ ends; RNA processing; RNA sequencing; RNA-seq; RNase; RNase cleavage; Transcription start site
Year: 2021 PMID： 34235668 DOI： 10.1007/978-1-0716-1460-0_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745