Relationship Between Serum Neopterin Level and Peripheral Arterial Plaque in Patients with Type 2 Diabetes.

OBJECTIVE: Neopterin is an inflammatory factor synthesized by monocyte macrophages in response to γ-interferon. It plays an important role in regulating a variety of physiological and pathological processes, including obesity, diabetes, and cardiovascular disease. This study aims to clarify the relationship between peripheral arterial plaque and serum neopterin in type 2 diabetes.
METHODS: We consecutively selected 121 inpatients with type 2 diabetes. After collecting relevant clinical indicators, we collected serum from all patients and measured neopterin levels through enzyme linked immune sorbent assay. Peripheral arterial plaques (the carotid and femoral arteries) were detected by B-mode ultrasound. Multivariate logistic regression was used to determine the independent influencing factors. The correlation between neopterin levels and other variables was analyzed by Spearman correlation analysis. P <0.05 was considered to be statistically significant.
RESULTS: There was no difference in serum neopterin levels between arterial plaques group and no plaques group. Serum neopterin levels in patients with carotid plaque were elevated compared to patients without carotid plaque. Logistic regression analysis showed that a higher serum neopterin level was an independent risk factor for the presence of carotid plaques. Serum neopterin levels were positively correlated with BMI, HOMA-IR, and serum creatinine and negatively correlated with eGFR.
CONCLUSION: Serum neopterin levels were positively and independently associated with carotid plaque in patients with type 2 diabetes.

Introduction

Along with the development of obesity, sedentary lifestyles, high-energy diets, and population aging, approximately 415 million adults worldwide had diabetes in 2015.1 Type 2 diabetes and its complications greatly exacerbate the burden of mortality and disability worldwide. Twenty-seven percentage of diabetics suffer from macrovascular complications,2 and macrovascular complications are the main cause of death in type 2 diabetes patients, accounting for 44% of all-cause mortality.3 Diabetes can cause atherosclerosis in all major vascular beds, including coronary, carotid, and lower extremity arteries.4 The mechanism of atherosclerosis in diabetes involves oxidative stress and inflammatory response of vascular endothelial cells.5 We have confirmed that visfatin serves as a risk factor for atherosclerotic plaques in type 2 diabetes.6 Studying the role of different inflammatory mediators in peripheral arterial plaques will help clarify the exact pathogenesis of macrovascular complications in type 2 diabetes.

Neopterin is an inflammatory factor synthesized and released by monocyte macrophages in response to γ-interferon during inflammation.7 Multiple studies have verified that neopterin could promote atherosclerosis. The review considers that neopterin is a risk marker for cardiovascular disease, whether it is for unstable angina, acute myocardial infarction, or cardiovascular death.8 Increased serum neopterin levels are associated with relapse of acute coronary syndrome and hospitalization of heart failure.9,10 In vivo, neopterin may promote plaque growth and apoptosis of vascular smooth muscle cells induced by oxidative stress.11 Regarding the relationship between neopterin and diabetes, the serum neopterin levels in patients with diabetes are significantly higher than in healthy and pre-diabetic people.12,13 Serum neopterin appears to be related to impaired insulin secretion and insulin resistance in the development of T2D.14,15 Although neopterin has been regarded as a proinflammatory and proatherosclerotic agent, more recent research indicated that neopterin also prevents both vascular inflammation and atherosclerosis and may be induced to counteract the progression of atherosclerotic lesions.16,17 Therefore, the correlation between circulating neopterin levels and peripheral plaques in different vascular territories in type 2 diabetes needs to be further investigated.

This study aimed to explore the association between serum neopterin and peripheral arterial plaque in patients with type 2 diabetes and to analyze the difference in neopterin levels in different vascular territories.

Methods

Study Population

This cross-sectional study of a single-center cohort was conducted for patients with type 2 diabetes at Changhai Hospital in Shanghai, China. Totally 121 inpatients were consecutively recruited from December 2016 to February 2018. We excluded patients younger than 18 years old, with type 1 diabetes, gestational diabetes, special types of diabetes, acute complications of diabetes (diabetic ketoacidosis and diabetic hyperosmolar coma), acute inflammatory diseases, malignancy, and other endocrine diseases. This study was authorized by the Ethics Committee of Changhai Hospital. The study was conducted in accordance with the Declaration of Helsinki. Written informed consent was obtained before the data collection and analysis.

Clinical and Biochemical Assessment

The basic information (age, gender, diabetes duration) and anthropometric measurements (height, body weight, waist circumference, hipline circumference) were collected according to standard measurement method. Laboratory data was measured from venous blood and urine samples, which were taken in the morning after an overnight fast of at least 10 h. Blood cell analysis (including white blood cell (WBC) count, total neutrophils, lymphocytes, and platelet count) was measured using the Sysmex XN-9000 analyzer (Sysmex Co., Kobe, Japan). Plasma glucose, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), bilirubin, total bile acid (TBA), blood urea nitrogen (BUN), serum creatinine, uric acid, estimated glomerular filtration rate (eGFR), lipid profile, including total cholesterol, triglycerides, HDL cholesterol (HDL-c), and LDL cholesterol (LDL-c) were performed on HITACHI 7600–120 automatic biochemical analyzer (Hitachi Co., Japan). Hemoglobin A1c (HbA1c) was measured by high-pressure liquid chromatography. Fasting blood glucose (FBG) and postprandial blood glucose (PBG) were measured by the glucose oxidase method. Insulin and C-peptide levels were measured with an automatic analyzer (Roche-E601, Roche Diagnostics, Germany). Urinary albumin-to-creatinine ratio (ACR) was measured using automatic urine microalbumin and creatinine analyzer.

Enzyme-Linked Immunosorbent Assay (ELISA)

Serum levels of neopterin were measured with commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions (Raybiotech Inc., GA, USA). The intra-assay and inter-assay coefficients of variation were less than 10% in enzyme immunoassays.

Peripheral Artery Ultrasound Measurement

B-mode ultrasound of the carotid and femoral arteries was performed with the Philips rE33 Ultrasound System (Philips Healthcare, Andover, MA). An experienced doctor who was blinded to clinical follow-up data accomplished the analysis of the presence of atheromatous plaques. Arterial plaques were defined as having an intima-media thickness (IMT) ≥1.3 mm or a focal protrusion into the lumen with a thickness of at least 50% more than the adjacent intima-media complex.

Definitions

The diagnostic criteria for diabetes are strictly based on American Diabetes Association standards. Diabetes duration is calculated from the time when they were first diagnosed with type 2 diabetes. Body mass index (BMI) is defined as body weight(kg)/height(m)2. Waist–hip ratio (WHR) is defined as waist circumference/hipline circumference. HOMA-IR is defined as fasting plasma glucose (mmol/L)* fasting insulin (mIU/L) /22.5. eGFR is calculated by the C-MDRD (modified for Chinese) equation: eGFR =175 x (Serum Creatinine) −1. 234 x (Age) −0.179 x (0.79 only if female).18

Statistical Analysis

Using SPSS 22.0 software, categorical data were expressed as proportion. Differences between groups were evaluated by χ2-test. Measurement data of normal distribution were expressed as mean ± standard deviation (SD), while skewed distribution data were shown as medians (Q1–Q3). Independent-sample t test or Mann–Whitney U-test was used for comparison between groups. Multivariate Logistic regression was used to determine the independent influencing factors. The association between serum neopterin levels and other variables was analyzed using Spearman correlation analysis. P values <0.05 were considered to be statistically significant.

The sample size calculations for the comparison between the mean of two independent groups were performed with PASS 11 (β=0.8, α=0.05, μβ=0.84, μα =1.96, s1=1.45, s2=0.68, δ=0.68). The minimal sample size required for each group was 45 subjects.

Results

General Characteristics of the Study Subjects

Overall, our study included 121 patients with T2DM who were admitted to our hospital. All patients were then divided into two groups according to the presence or absence of carotid and/or femoral arteries plaques. No plaques in the carotid and/or femoral arteries were found in 41.3% (50/121) of patients. Unexpectedly, as shown in , there was no difference in serum neopterin levels between these two groups. Serum neopterin also cannot serve as a predictor of plaques ().

Comparison of Clinical Indicators and Neopterin Concentrations in Carotid Plaque Group and No Carotid Plaque Group

Considering the different pathogenesis of atherosclerotic plaques in different vascular territories, we further analyzed the relationship between serum neopterin levels with plaques in different arterial territories. All patients were then divided into two groups according to the presence or absence of carotid plaques: carotid plaque group (48 subjects) and no carotid plaque group (73 subjects). As shown in Table 1 and , age was higher in the group with carotid plaques. By contrast, eGFR, serum direct bilirubin, and indirect bilirubin were significantly lower in the group with carotid plaques. Furthermore, serum neopterin levels were elevated in the group with carotid plaques (P=0.026).

Table 1

Comparison of Carotid Plaque Group and No Carotid Plaque Group

Variables	Carotid Plaque (n=48)	No Carotid Plaque (n=73)	P-value
Age(years)	58.63±9.68	48.55±13.81	<0.001
Gender(male)	30(62.5%)	52(71.2%)	0.315
Diabetes duration(years)	6(1.25–10)	3(1–9)	0.146a
BMI(kg/m2)	27.89±3.03	27.77±4.26	0.861
Waist-to-hip ratio	0.96±0.07	0.96±0.06	0.839
Indirect bilirubin(μmol/L)	7.51±3.12	9.68±4.42	0.002
Direct bilirubin(μmol/L)	3.38±1.41	4.21±2.33	0.016
Serum creatinine(μmol/L)	75.50±21.75	69.07±18.63	0.085
eGFR(mL/min)	94.13±25.32	110.95±27.21	0.001
Total cholesterol(mmol/L)	4.68±1.51	5.36±2.21	0.064
Triglycerides(mmol/L)	1.72(1.27–2.86)	1.57(1.20–3.09)	0.761a
HDL cholesterol(mmol/L)	1.00±0.22	1.09±0.34	0.070
LDL cholesterol(mmol/L)	2.75±1.05	2.87±1.22	0.565
ACR(mg/g)	17.5(5.25–134.25)	10(5–41)	0.300a
Fasting plasma glucose(mmol/L)	9.19±2.90	9.47±3.22	0.630
1h postprandial plasma glucose(mmol/L)	15.90±4.08	16.10±3.89	0.782
2h postprandial plasma glucose(mmol/L)	18.23±4.01	17.86±4.50	0.646
Fasting insulin(mIU/L)	13.1(6.85–18.95)	11.4(7.05–17.0)	0.433a
Fasting C-peptide(μg/L)	2.37(1.82–3.34)	2.50(1.56–3.13)	0.649a
1h postprandial insulin(mIU/L)	25.3(16.05–56.95)	28.0(13.15–45.2)	0.578a
1h postprandial C-peptide(μg/L)	3.62(2.72–5.96)	3.35(2.36–4.97)	0.395a
2 h postprandial insulin(mIU/L)	31.25(13.68–56.28)	29.3(15.35–56.35)	0.801a
2 h postprandial C-peptide(μg/L)	4.59(3.38–7.17)	4.78(3.04–6.53)	0.494a
HOMA-IR	4.68(3.25–8.33)	4.62(3.00–8.06)	0.468a
Hemoglobin A1c(%)	9.61±1.89	9.53±2.18	0.852
Neopetrin(ng/mL)	2.02(1.57–2.89)	1.80(1.48–2.26)	0.026a

Notes: acompared by the Mann–Whitney test.

Abbreviations: BMI, body mass index; eGFR, estimated glomerular filtration rate; HDL cholesterol, high-density lipoprotein cholesterol; LDL cholesterol, low-density lipoprotein cholesterol; ACR, urinary albumin-to-creatinine ratio.

Serum Neopterin as a Predictor of Carotid Plaques

Multivariable logistic regression analysis was performed in order to assess the independent predictors of the presence of carotid plaques. The above 5 statistically different variables (age, eGFR, serum direct bilirubin, indirect bilirubin, and neopterin) were entered into the full regression model. As demonstrated in Table 2, after controlling for confounding factors, older age, and higher serum neopterin levels were independent risk factors for the presence of carotid plaques. The OR-value of neopterin is 1.689 (1.006, 2.834).

Table 2

Independent Factors for Carotid Plaques by Multivariable Logistic Regression Analysis

Parameters	OR (95% CI)	P-value
Age	1.061(1.020,1.104)	0.003
Direct bilirubin	0.853(0.578,1.258)	0.422
Indirect bilirubin	0.908(0.758,1.087)	0.293
eGFR	0.990(0.973,1.007)	0.246
Neopterin	1.689(1.006,2.834)	0.047

Abbreviations: OR, odds ratio; CI, confidence interval; eGFR, estimated glomerular filtration rate.

Comparison of Clinical Indicators and Neopterin Concentrations in Femoral Arterial Plaque Group and No Femoral Arterial Plaque Group

Then, according to the B-mode ultrasonic examination of femoral arteries, they were classified into two groups: femoral arterial plaque group (52 subjects) and no femoral arterial plaque group (69 subjects). As shown in Table 3 and , age and diabetes duration were higher in femoral arterial plaque group. On the other hand, serum indirect bilirubin, alanine aminotransferase, aspartate aminotransferase, γ-Glutamyl transpeptidase, and total cholesterol were significantly lower in femoral arterial plaque group. There was no statistical difference in serum neopterin levels between the two groups (P=0.130). Multivariable logistic regression analysis revealed that serum neopterin levels were not independent risk factors for the presence of femoral plaques ().

Table 3

The Comparison of Femoral Plaque Group and No Femoral Plaque Group

Variables	Femoral Plaque (n=52)	No Femoral Plaque (n=69)	P-value
Age(years)	58.31±10.38	48.20±13.58	<0.001
Gender(male)	38(73.1%)	44(63.8%)	0.278
Diabetes duration(years)	6(2–11.75)	3(0.54–8)	0.027a
BMI(kg/m2)	27.36±2.79	28.16±4.41	0.228
Waist-to-hip ratio	0.96±0.06	0.96±0.07	0.941
Indirect bilirubin(μmol/L)	7.92±2.93	9.49±4.68	0.026
Direct bilirubin(μmol/L)	3.51±1.17	4.17±2.49	0.058
Serum creatinine(μmol/L)	74.29±21.62	69.61±18.76	0.206
eGFR(mL/min)	98.99±26.58	108.26±27.93	0.068
Total cholesterol(mmol/L)	4.56±1.47	5.49±2.23	0.010
Triglycerides(mmol/L)	1.62(1.15–2.86)	1.77(1.26–3.35)	0.316a
HDL cholesterol(mmol/L)	1.03±0.33	1.08±0.28	0.435
LDL cholesterol(mmol/L)	2.61±1.10	2.98±1.17	0.084
ACR(mg/g)	12(4.25–126.75)	11(5–45.57)	0.755a
Fasting plasma glucose(mmol/L)	8.91±2.49	9.70±3.45	0.165
1h postprandial plasma glucose(mmol/L)	15.96±3.75	16.07±4.12	0.889
2h postprandial plasma glucose(mmol/L)	18.14±3.85	17.91±4.64	0.777
Fasting insulin(mIU/L)	12.1(6.78–18.95)	12.2(7.4–17.6)	0.900a
Fasting C-peptide(μg/L)	2.47(1.77–3.14)	2.41(1.61–3.20)	0.992a
1h postprandial insulin(mIU/L)	28.7(19.0–63.08)	23.8(11.85–45.2)	0.079a
1h postprandial C-peptide(μg/L)	3.67(2.44–5.80)	3.32(2.38–4.91)	0.250a
2 h postprandial insulin(mIU/L)	30.95(17.55–61.93)	26.4(13.2–50.15)	0.124a
2 h postprandial C-peptide(μg/L)	4.75(3.33–7.13)	4.56(2.97–6.53)	0.308a
HOMA-IR	4.55(2.92–7.98)	4.80(3.10–8.83)	0.755a
Hemoglobin A1c(%)	9.32±1.86	9.75±2.20	0.261
Neopetrin(ng/mL)	2.00(1.56–2.71)	1.80(1.51–2.28)	0.130a

Notes: acompared by the Mann–Whitney test.

Abbreviations: BMI, body mass index; eGFR, estimated glomerular filtration rate; HDL cholesterol, high-density lipoprotein cholesterol; LDL cholesterol, low-density lipoprotein cholesterol; ACR, urinary albumin-to-creatinine ratio.

Correlations Between Serum Neopterin Levels and Clinical Indicators

In order to find out the relevant factors that affect the serum neopterin levels in patients with type 2 diabetes, the correlation between the serum neopterin levels and other clinical parameters was analyzed by Spearman correlation analysis. As shown in Table 4 and , serum neopterin levels were positively correlated with BMI (rs=0.191, P=0.036), serum creatinine (rs=0.327, P<0.001), HOMA-IR (rs=0.184, P=0.043), and negatively correlated with eGFR (rs=–0.358, P<0.001).

Table 4

Correlation of Neopterin Levels and Clinical Variables

Parameters	Neopterin
	rs	P-value
BMI	0.191	0.036
Serum creatinine	0.327	<0.001
eGFR	–0.358	<0.001
HOMA-IR	0.184	0.043

Abbreviations: BMI, body mass index; eGFR, estimated glomerular filtration rate.

Discussion

Neopterin is an inflammatory factor secreted by monocyte macrophages.7 In the past two decades, neopterin has received more and more attention in the pathogenesis of cardiovascular complications.19–21 In diabetic patients, it is also confirmed that neopterin is an independent predictor for fatal ischemic heart disease.22

Diabetes mellitus is a major risk factor for peripheral artery disease. In this study, we tried to establish an association between peripheral arterial plaques, as detected by ultrasonography, in two different territories, including the carotid and femoral arteries, and the serum neopterin levels. According to our results, serum neopterin levels in type 2 diabetes patients with carotid plaque were significantly higher than group without carotid plaque. Interestingly, there was no statistical difference in the levels of neopterin between femoral arterial plaque group and no femoral arterial plaque group. The difference between the carotid arterial plaque group and the femoral arterial plaque group may attribute to the plaque composition. The area and number of macrophages in carotid arterial plaque are higher than those in femoral arterial plaque,23 while neopterin is secreted by activated monocyte macrophages. Except for macrophages, the distribution of other inflammatory molecular markers is different in carotid and femoral atherosclerotic plaques.24 Regional differences in the hemodynamic profile prime the endothelial phenotype to respond distinctly to such systemic risk factors as hypercholesterolemia, genetics, immune status, and oxidative stress.25 So neopterin may play a unique role in the process of carotid atherosclerosis, which is different from other vascular territory.

Briefly, after controlling for other confounding factors, neopterin was still an independent predictor for carotid plaque in patients with type 2 diabetes. Migration of monocyte macrophages through the endothelium represents a key step of atherosclerosis, and the relationship between neopterin and atherosclerosis could be partly explained by the associated activation of macrophages. Oxidative stress seems to play a vital role,26 because neopterin amplifies the activation of reactive oxygen species mediated cellular processes.27 De Rosa et al held that activated monocyte macrophages in local atherosclerotic areas obviously increase neopterin levels, thus inducing the expression of adhesion molecules in endothelial cells. These adhesion molecules result in increased binding of lymphocytes, platelets, and other monocytes. Subsequent endothelial activation causes expression of tissue factor, finally leading to the occurrence of atherosclerosis.28 Nevertheless, the specific molecular mechanisms involved in carotid arterial plaques need to be further studied.

Neopterin is excreted through the kidneys in vivo.29 We also found that serum neopterin levels were positively correlated with serum creatinine, and negatively correlated with eGFR in patients with type 2 diabetes. Similarly, a prospective cohort study confirmed that the serum creatinine of high-level neopterin increased faster than low-level neopterin group in diabetic nephropathy patients.30 Spearman correlation analysis showed positive correlations between neopterin levels and BMI as well. Neopterin levels in obese people were higher than in non-obese people,31 and the level of neopterin decreased after weight loss.32 Likewise, subjects with WHR >0.9 had higher serum neopterin levels.33 The association between neopterin and BMI may ascribe to chronic low-grade inflammation in obese people.34 In this study, we revealed that serum neopterin levels were positively correlated with HOMA-IR, which is consistent with the previous report performed in newly diagnosed patients with T2D who did not take any hypoglycemic agents.14

In addition, there are several limitations to be discussed. First of all, our study was based on cross-sectional data with a small sample size from a single center. Prospective studies with larger samples and multiple centers are needed to explore the role of serum neopterin in carotid and lower extremity arterial plaques of type 2 diabetes. Secondly, we did not collect the history of drug therapy, such as insulin, oral hypoglycemic drugs, and other factors which may have impact on atherosclerosis. Finally, atherosclerosis has a complicated pathological process, and it involves many molecular markers. Only one biomarker is not enough to view the whole picture of atherosclerotic plaque formation.

Conclusions

We underline that serum neopterin levels were positively and independently associated with carotid plaque in patients with type 2 diabetes. These results also imply that neopterin could be a new therapeutic target both for the prevention and the treatment of carotid plaque.