Napachanok Mongkoldhumrongkul Swainson1, Pongsakorn Aiemderm1, Chonnikarn Saikaew2,3, Kanyanat Theeraraksakul2, Pakjira Rimdusit4, Charoenkwan Kraiya5, Sasimanas Unajak1, Kiattawee Choowongkomon6,7. 1. Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Road, Bangkok, 10900, Chatuchak, Thailand. 2. Genetic Engineering Interdisciplinary Program, Graduate School, Kasetsart University, 50 Ngam Wong Wan Rd, Bangkok, 10900, Chatuchak, Thailand. 3. Division of Occupational and Environmental Diseases, Department of Disease Control, Saraburi, 18120, Thailand. 4. Office of Disease Prevention and Control 4, Department of Disease Control, Saraburi, 18120, Thailand. 5. Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand. 6. Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Road, Bangkok, 10900, Chatuchak, Thailand. fsciktc@ku.ac.th. 7. Omics Center for Agriculture, Bioresources, Food and Health, Kasetsart University, Bangkok, Thailand. fsciktc@ku.ac.th.
Abstract
OBJECTIVE: An aptamer specifically binding to diethyl thiophosphate (DETP) was constructed and incorporated in an optical sensor and electrochemical techniques to enable the specific measurement of DETP as a metabolite and a biomarker of organophosphate exposure. RESULTS: A DETP-bound aptamer was selected from the library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX). A colorimetric method revealed that the aptamer had the highest affinity for DETP, with a mean Kd value (± SD) of 0.103 ± 0.014 µM. The docking results and changes in resistance showed that the selectivity of the aptamer for DETP was higher than that for the similar structures of dithiophosphate (DEDTP) and diethyl phosphate (DEP). The altered amplitude of cyclic voltammetry showed a linear range of DETP detection covering 0.0001-10 µg/ml with a limit of detection of 0.007 µg/ml. The recovery value of a real sample of pH 7 was 97.2%. CONCLUSIONS: The current method showed great promise in using the DETP-specific aptamer to detect the exposure history to organophosphates by measuring their metabolites, although degradation of organophosphate parent compounds might occur.
OBJECTIVE: An aptamer specifically binding to diethyl thiophosphate (DETP) was constructed and incorporated in an optical sensor and electrochemical techniques to enable the specific measurement of DETP as a metabolite and a biomarker of organophosphate exposure. RESULTS: A DETP-bound aptamer was selected from the library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX). A colorimetric method revealed that the aptamer had the highest affinity for DETP, with a mean Kd value (± SD) of 0.103 ± 0.014 µM. The docking results and changes in resistance showed that the selectivity of the aptamer for DETP was higher than that for the similar structures of dithiophosphate (DEDTP) and diethyl phosphate (DEP). The altered amplitude of cyclic voltammetry showed a linear range of DETP detection covering 0.0001-10 µg/ml with a limit of detection of 0.007 µg/ml. The recovery value of a real sample of pH 7 was 97.2%. CONCLUSIONS: The current method showed great promise in using the DETP-specific aptamer to detect the exposure history to organophosphates by measuring their metabolites, although degradation of organophosphate parent compounds might occur.
Authors: Pornpimol Kongtip; Benyachalee Techasaensiri; Noppanun Nankongnab; Jane Adams; Akkarat Phamonphon; Anu Surach; Supha Sangprasert; Aree Thongsuksai; Prayoon Srikumpol; Susan Woskie Journal: Int J Environ Res Public Health Date: 2017-05-27 Impact factor: 3.390
Authors: Sailent Rizki Sari Simaremare; Chien-Che Hung; Chia-Jung Hsieh; Lih-Ming Yiin Journal: Int J Environ Res Public Health Date: 2019-12-18 Impact factor: 3.390