Hiroshi Hyakusoku1,2, Kae Sawakuma1, Daisuke Sano3,4, Hideaki Takahashi1, Takashi Hatano1, Kaname Sato1, Yasuhiro Isono1, Shoko Shimada1, Kentaro Takada1, Tatsu Kuwahara1, Yoshihiro Aizawa1, Nobuhiko Oridate1,4. 1. Department of Biology and Function in Head and Neck, Yokohama City University Graduate School of Medicine, Yokohama, Japan. 2. Department of Otorhinolaryngology, Yokosuka Kyosai Hospital, Yokosuka, Japan. 3. Department of Biology and Function in Head and Neck, Yokohama City University Graduate School of Medicine, Yokohama, Japan; dsano@yokohama-cu.ac.jp. 4. Department of Otorhinolaryngology - Head and Neck Surgery, Yokohama City University, School of Medicine, Yokohama, Japan.
Abstract
BACKGROUND/AIM: We evaluated the impact of FosL1, a member of the activated protein-1 family, on the pathways leading to regional metastasis of head and neck squamous cell carcinoma (HNSCC). MATERIALS AND METHODS: We examined the influence of small interfering RNA (siRNA) and short heparin RNA (shRNA) mediated knockdown of FosL1 on cell migration, invasion, and proliferation in vitro as well as on regional metastasis in vivo. The prognostic significance of FosL1 was also analyzed using the Kaplan- Meier plotter using data from an HNSCC patient database. RESULTS: Down-regulation of FosL1 inhibited cell migration, invasion, and proliferation in vitro, decreased the incidence of regional metastases, and prolonged the survival of mice in vivo. We also determined that HNSCC patients with higher expression levels of FosL1 had a significantly shorter survival time than those with low expression of FosL1. CONCLUSION: FosL1 plays a crucial role in promoting cell migration, invasion, and proliferation in HNSCC.
BACKGROUND/AIM: We evaluated the impact of FosL1, a member of the activated protein-1 family, on the pathways leading to regional metastasis of head and neck squamous cell carcinoma (HNSCC). MATERIALS AND METHODS: We examined the influence of small interfering RNA (siRNA) and short heparin RNA (shRNA) mediated knockdown of FosL1 on cell migration, invasion, and proliferation in vitro as well as on regional metastasis in vivo. The prognostic significance of FosL1 was also analyzed using the Kaplan- Meier plotter using data from an HNSCC patient database. RESULTS: Down-regulation of FosL1 inhibited cell migration, invasion, and proliferation in vitro, decreased the incidence of regional metastases, and prolonged the survival of mice in vivo. We also determined that HNSCC patients with higher expression levels of FosL1 had a significantly shorter survival time than those with low expression of FosL1. CONCLUSION:FosL1 plays a crucial role in promoting cell migration, invasion, and proliferation in HNSCC.