A Dunislawska1, A Slawinska2, M Gryzinska3, M Siwek2. 1. Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, 85-084, Bydgoszcz, Poland. aleksandra.dunislawska@utp.edu.pl. 2. Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, 85-084, Bydgoszcz, Poland. 3. Institute of Biological Basis of Animal Production, Sub-Department of General and Molecular Genetics, University of Life Sciences in Lublin, 20-032, Lublin, Poland.
Abstract
BACKGROUND: Epigenetic regulation of the gene expression results from interaction between the external environment and transcription of the genetic information encoded in DNA. Methylated CpG regions within the gene promoters lead to silencing of the gene expression in most cases. Factors contributing to epigenetic regulation include intestinal microbiota, which in chicken can be potently modified by in ovo stimulation. The main aim of this study was to determine global and specific methylation patterns of the spleen under the influence of host-microbiome interaction. RESULTS: Fertilized eggs of two genotypes: Ross 308 and Green-legged Partridgelike were in ovo stimulated on d 12 of incubation. The injected compounds were as follows: probiotic - Lactococcus lactis subsp. cremoris IBB477, prebiotic - galactooligosaccharides, and synbiotic - combination of both. Chickens were sacrificed on d 42 post-hatching. Spleen was collected, RNA and DNA were isolated and intended to gene expression, gene methylation and global methylation analysis. We have proved that negative regulation of gene expression after administration of bioactive substances in ovo might have epigenetic character. Epigenetic changes depend on the genotype and the substance administered in ovo. CONCLUSION: Epigenetic nature of microbial reprogramming in poultry and extension of issues related to host-microbiome interaction is a new direction of this research.
BACKGROUND: Epigenetic regulation of the gene expression results from interaction between the external environment and transcription of the genetic information encoded in DNA. Methylated CpG regions within the gene promoters lead to silencing of the gene expression in most cases. Factors contributing to epigenetic regulation include intestinal microbiota, which in chicken can be potently modified by in ovo stimulation. The main aim of this study was to determine global and specific methylation patterns of the spleen under the influence of host-microbiome interaction. RESULTS: Fertilized eggs of two genotypes: Ross 308 and Green-legged Partridgelike were in ovo stimulated on d 12 of incubation. The injected compounds were as follows: probiotic - Lactococcus lactis subsp. cremoris IBB477, prebiotic - galactooligosaccharides, and synbiotic - combination of both. Chickens were sacrificed on d 42 post-hatching. Spleen was collected, RNA and DNA were isolated and intended to gene expression, gene methylation and global methylation analysis. We have proved that negative regulation of gene expression after administration of bioactive substances in ovo might have epigenetic character. Epigenetic changes depend on the genotype and the substance administered in ovo. CONCLUSION: Epigenetic nature of microbial reprogramming in poultry and extension of issues related to host-microbiome interaction is a new direction of this research.
Authors: Joseph Hargan-Calvopina; Sara Taylor; Helene Cook; Zhongxun Hu; Serena A Lee; Ming-Ren Yen; Yih-Shien Chiang; Pao-Yang Chen; Amander T Clark Journal: Dev Cell Date: 2016-09-09 Impact factor: 12.270
Authors: Aleksandra Dunislawska; Anna Slawinska; Katarzyna Stadnicka; Marek Bednarczyk; Piotr Gulewicz; Damian Jozefiak; Maria Siwek Journal: PLoS One Date: 2017-01-03 Impact factor: 3.240