João Miguel Santos1, Joana A Marques2, Patrícia Diogo3, Ana Messias4, Vítor Sousa5, Diana Sequeira6, Paulo J Palma3. 1. Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075; Center for Innovation and Research in Oral Sciences (CIROS), Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Center for Neuroscience and Cell Biology (CNC), University of Coimbra, 3004- 504 Coimbra, Portugal. Electronic address: jsantos@fmed.uc.pt. 2. Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075. 3. Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075; Center for Innovation and Research in Oral Sciences (CIROS), Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal. 4. Center for Innovation and Research in Oral Sciences (CIROS), Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Institute of Oral Implantology and Prosthodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal. 5. Institute of Pathological Anatomy, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal. 6. Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075; Center for Neuroscience and Cell Biology (CNC), University of Coimbra, 3004- 504 Coimbra, Portugal.
Abstract
INTRODUCTION: This study aims to evaluate the impact of pre-operative pulp inflammation on the histologic outcome of full pulpotomy performed in mature permanent posterior teeth, using four different biomaterials.; METHODS: Five beagle dogs (providing a total of 120 roots) were selected. Dentin exposure was performed in teeth from second and third quadrants. One week later, full pulpotomy procedures were carried out using four different bioactive materials (ProRoot MTA, TotalFill BC Putty (BC), Biodentine (BIO) and an experimental cement - PCM). Hemostasis time was registered. After 14 weeks, animals were killed. Pulp-dentin tissues were histologically and radiographically assessed. Significance level was set at .05.; RESULTS: Teeth with previously exposed dentin revealed a statistically significant increase in time required to achieve hemostasis (p < .001), therefore confirming the pulp inflammation status induced by one-week exposure of occlusal dentin before performing full pulpotomy. No radiographic evidence of root resorption, periapical radiolucency or lamina dura alterations. No statistically significant differences were observed between normal and inflamed pulps, regardless of the evaluated histological parameters. Moreover, histological data concerning calcified barrier formation and pulp tissue response shows better results for BIO, without statistical differences when compared to MTA or BC (p > .05). PCM presented lower performance, with statistically significant differences being detected in regard to the remaining three tested materials (p < .001).; CONCLUSIONS: Radiographic and histologic outcomes of full pulpotomy are not jeopardized by short-term pre-operative pulp inflammation. Moreover, all BIO, MTA and BC cements present suitable alternatives to be used as pulp capping agents.
INTRODUCTION: This study aims to evaluate the impact of pre-operative pulp inflammation on the histologic outcome of full pulpotomy performed in mature permanent posterior teeth, using four different biomaterials.; METHODS: Five beagle dogs (providing a total of 120 roots) were selected. Dentin exposure was performed in teeth from second and third quadrants. One week later, full pulpotomy procedures were carried out using four different bioactive materials (ProRoot MTA, TotalFill BC Putty (BC), Biodentine (BIO) and an experimental cement - PCM). Hemostasis time was registered. After 14 weeks, animals were killed. Pulp-dentin tissues were histologically and radiographically assessed. Significance level was set at .05.; RESULTS: Teeth with previously exposed dentin revealed a statistically significant increase in time required to achieve hemostasis (p < .001), therefore confirming the pulp inflammation status induced by one-week exposure of occlusal dentin before performing full pulpotomy. No radiographic evidence of root resorption, periapical radiolucency or lamina dura alterations. No statistically significant differences were observed between normal and inflamed pulps, regardless of the evaluated histological parameters. Moreover, histological data concerning calcified barrier formation and pulp tissue response shows better results for BIO, without statistical differences when compared to MTA or BC (p > .05). PCM presented lower performance, with statistically significant differences being detected in regard to the remaining three tested materials (p < .001).; CONCLUSIONS: Radiographic and histologic outcomes of full pulpotomy are not jeopardized by short-term pre-operative pulp inflammation. Moreover, all BIO, MTA and BC cements present suitable alternatives to be used as pulp capping agents.
Authors: Amjad Abu Hasna; Ana Luisa Theodoro; Larissa Marques Pereira; Lucas de Paula Ramos; Tiago Moreira Bastos Campos; Maisour Ala Rachi; Talal Al-Nahalwi; Luciane Dias de Oliveira; Cláudio Antonio Talge Carvalho Journal: Biomed Res Int Date: 2022-05-10 Impact factor: 3.246
Authors: Flavia Iaculli; Francisco Javier Rodríguez-Lozano; Benjamín Briseño-Marroquín; Thomas Gerhard Wolf; Gianrico Spagnuolo; Sandro Rengo Journal: J Clin Med Date: 2022-07-11 Impact factor: 4.964