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Literature DB >> 3421946

Methylation of the alpha 2(I) collagen gene in chemically transformed rat liver epithelial cells.

Abstract

W8 cells, 2-N-(acetoxyacetyl)aminofluorene-transformed rat liver epithelial-like cells, secrete no alpha 2(I) collagen chains. This paper reports the first demonstration of DNA methylation in the promoter-5' region of an alpha 2(I) collagen gene which occurs in W8 cells. Since inhibition of methylation by azacytidine induces transcription of the alpha 2(I) gene, DNA methylation of W8 alpha 2(I) promoter-5' region could contribute to altered collagen production in these cells.

Entities: Chemical Species

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Year: 1988 PMID： 3421946 PMCID： PMC1149285 DOI： 10.1042/bj2530269

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

28 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas.

Authors: I B Weinstein; J M Orenstein; R Gebert; M E Kaighn; U C Stadler
Journal: Cancer Res Date: 1975-01 Impact factor: 12.701

3. Identification of collagen alpha1(I) trimer and normal type I collagen in a polyoma virus-induced mouse tumor.

Authors: L Moro; B D Smith
Journal: Arch Biochem Biophys Date: 1977-07 Impact factor: 4.013

4. Specific changes in the collagen phenotype of BALB 3T3 cells as a result of transformation by sarcoma viruses or a chemical carcinogen.

Authors: R I Hata; B Peterkofsky
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

5. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors: S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

6. Methylation of type II and type I collagen genes in differentiated and dedifferentiated chondrocytes.

Authors: M P Fernández; M F Young; M E Sobel
Journal: J Biol Chem Date: 1985-02-25 Impact factor: 5.157

7. Synthesis of collagen by mammalian cell lines of fibroblastic and nonfibroblastic origin.

Authors: H Green; B Goldberg
Journal: Proc Natl Acad Sci U S A Date: 1965-06 Impact factor: 11.205

8. Construction of DNA sequences complementary to rat alpha 1 and alpha 2 collagen mRNA and their use in studying the regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D.

Authors: C Genovese; D Rowe; B Kream
Journal: Biochemistry Date: 1984-12-04 Impact factor: 3.162

9. Simultaneous synthesis of types I and III collagen by fibroblasts in culture.

Authors: S Gay; G R Martin; P K Muller; R Timpl; K Kuhn
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

10. Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine.

Authors: R Mayne; M S Vail; E J Miller
Journal: Proc Natl Acad Sci U S A Date: 1975-11 Impact factor: 11.205

5 in total

Methylation of the alpha 2(I) collagen gene in chemically transformed rat liver epithelial cells.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas.

3. Identification of collagen alpha1(I) trimer and normal type I collagen in a polyoma virus-induced mouse tumor.

4. Specific changes in the collagen phenotype of BALB 3T3 cells as a result of transformation by sarcoma viruses or a chemical carcinogen.

5. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

6. Methylation of type II and type I collagen genes in differentiated and dedifferentiated chondrocytes.

7. Synthesis of collagen by mammalian cell lines of fibroblastic and nonfibroblastic origin.

8. Construction of DNA sequences complementary to rat alpha 1 and alpha 2 collagen mRNA and their use in studying the regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D.

9. Simultaneous synthesis of types I and III collagen by fibroblasts in culture.

10. Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine.

1. DNA methylation inhibits transcription of procollagen alpha 2(I) promoters.

2. Developmental changes in the methylation status of regulatory elements in the murine alpha 1(I) collagen gene.

3. DNA methylation represses the murine alpha 1(I) collagen promoter by an indirect mechanism.

4. Collagen alpha1(I) gene (COL1A1) is repressed by RFX family.

5. Regulation of the human alpha 2(1) procollagen gene by sequences adjacent to the CCAAT box.