Literature DB >> 3421721

Self-association of hyaluronate segments in aqueous NaCl solution.

R E Turner1, P Y Lin, M K Cowman.   

Abstract

The potential for self-association by hyaluronate (HA) chains in 0.15 M NaCl was investigated, using low molecular weight HA segments as a model system. HA segments were derived from the polymer by controlled enzymatic digestion, and purified by gel filtration chromatography. Seven samples of narrow molecular weight distribution were analyzed by sensitivity-enhanced polyacrylamide gel electrophoresis, and found to have the following weight-average numbers of repeating disaccharide units: A, 90; B, 51; C, 38; D, 31; E, 23; F, 18; G, 13. The segment preparations were studied in 0.15 M NaCl by capillary viscometry, low angle laser light scattering, and circular dichroism spectroscopy. The data indicate concentration-dependent intermolecular association of short segments, and a capability for intramolecular association (hairpin formation) by larger HA segments.

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Year:  1988        PMID： 3421721     DOI： 10.1016/0003-9861(88)90153-1

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  10 in total

1.  Hyaluronan forms specific stable tertiary structures in aqueous solution: a 13C NMR study.

Authors:  J E Scott; F Heatley
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-27       Impact factor: 11.205

Review 2.  Hyaluronidases: their genomics, structures, and mechanisms of action.

Authors:  Robert Stern; Mark J Jedrzejas
Journal:  Chem Rev       Date:  2006-03       Impact factor: 60.622

3.  Tapping mode atomic force microscopy of hyaluronan: extended and intramolecularly interacting chains.

Authors:  M K Cowman; M Li; E A Balazs
Journal:  Biophys J       Date:  1998-10       Impact factor: 4.033

4.  Hyaluronan: the absence of amide-carboxylate hydrogen bonds and the chain conformation in aqueous solution are incompatible with stable secondary and tertiary structure models.

Authors:  Charles D Blundell; Paul L Deangelis; Andrew Almond
Journal:  Biochem J       Date:  2006-06-15       Impact factor: 3.857

5.  Evidence of a defined spatial arrangement of hyaluronate in the central filament of cartilage proteoglycan aggregates.

Authors:  M Mörgelin; M Paulsson; D Heinegård; U Aebi; J Engel
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857

6.  Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan.

Authors:  Shardul Bhilocha; Ripal Amin; Monika Pandya; Han Yuan; Mihir Tank; Jaclyn LoBello; Anastasia Shytuhina; Wenlan Wang; Hans-Georg Wisniewski; Carol de la Motte; Mary K Cowman
Journal:  Anal Biochem       Date:  2011-05-27       Impact factor: 3.365

7.  Determination of hyaluronan molecular mass distribution in human breast milk.

Authors:  Han Yuan; Ripal Amin; Xin Ye; Carol A de la Motte; Mary K Cowman
Journal:  Anal Biochem       Date:  2015-01-09       Impact factor: 3.365

8.  Extended, relaxed, and condensed conformations of hyaluronan observed by atomic force microscopy.

Authors:  Mary K Cowman; Chiara Spagnoli; Dina Kudasheva; Min Li; Ansil Dyal; Sonoko Kanai; Endre A Balazs
Journal:  Biophys J       Date:  2004-10-15       Impact factor: 4.033

9.  Isolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan.

Authors:  J M Ponting; S Kumar
Journal:  J Anat       Date:  1995-02       Impact factor: 2.610

10.  Molecular mass dependence of hyaluronan detection by sandwich ELISA-like assay and membrane blotting using biotinylated hyaluronan binding protein.

Authors:  Han Yuan; Mihir Tank; Abeer Alsofyani; Naman Shah; Nishant Talati; Jaclyn C LoBello; Jin Ryoun Kim; Yoji Oonuki; Carol A de la Motte; Mary K Cowman
Journal:  Glycobiology       Date:  2013-08-19       Impact factor: 4.313
  10 in total

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