C Silverio-Gómez1, J Vega-Arreguín2, G Nic-Matos1, M Narváez-Cab1, L Sáenz-Carbonell1, C Oropeza3. 1. Centro de Investigación Científica de Yucatán A. C., Unidad de Biotecnología, Calle 43 No. 130 x 32 y 34., Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico. 2. UNAM: Escuela Nacional de Estudios Superiores Unidad León (ENES-León), Ciencias Agrogenómicas, Blv. UNAM #2011, Predio El Saucillo y, Comunidad de los Tepetates, El Potrero, C.P. 37684, León, Guanajuato, Mexico. 3. Centro de Investigación Científica de Yucatán A. C., Unidad de Biotecnología, Calle 43 No. 130 x 32 y 34., Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico. cos@cicy.mx.
Abstract
BACKGROUND: Salicylic acid (SA) is an important regulator of genes involved in plant defense and pathogen-triggered systemic acquired resistance (SAR). Coconut is an important crop affected by several pathogens. Reported evidence suggests SA involvement in defense responses, including SAR in coconut. OBJECTIVE: To identified differentially expressed genes in leaf and root tissues of coconut plantlets, as a result of SA, that might be involved in coconut defense responses. METHODS: Comparative transcriptomic analysis by RNA-Seq of leaf and root tissues from in vitro coconut plantlets unexposed and exposed to SA 2.5 mM for 48 h. And in silico validation of gene expression by qRT-PCR. RESULTS: We identified 4615 and 3940 differentially expressed unigenes (DEUs) in leaf and root tissues respectively. Our GO analysis showed functional categories related to the induction of defense responses, such as "systemic acquired resistance" and highly enriched hormone categories, such as abscisic acid. The most abundant KEGG pathway in our results was "Biosynthesis of antibiotics". Our findings support that exogenous application of SA to plantlets induced the activation of PRs, RGAs, ICS2, NLTP2, PER4, TRXM and some WRKYs mediated by NPR1-dependent pathways. Also, we found DEUs, such as BZR1, HSL1, and WHY2 that support that SA could regulate defense-related genes through NPR1-independent pathways. CONCLUSION: The present study of massive data analysis carried out on coconut plantlets exposed to SA, generates valuable information that increases our understanding of defense molecular mechanisms in coconut and open new venues for research for the improvement of management of coconut diseases.
BACKGROUND: Salicylic acid (SA) is an important regulator of genes involved in plant defense and pathogen-triggered systemic acquired resistance (SAR). Coconut is an important crop affected by several pathogens. Reported evidence suggests SA involvement in defense responses, including SAR in coconut. OBJECTIVE: To identified differentially expressed genes in leaf and root tissues of coconut plantlets, as a result of SA, that might be involved in coconut defense responses. METHODS: Comparative transcriptomic analysis by RNA-Seq of leaf and root tissues from in vitro coconut plantlets unexposed and exposed to SA 2.5 mM for 48 h. And in silico validation of gene expression by qRT-PCR. RESULTS: We identified 4615 and 3940 differentially expressed unigenes (DEUs) in leaf and root tissues respectively. Our GO analysis showed functional categories related to the induction of defense responses, such as "systemic acquired resistance" and highly enriched hormone categories, such as abscisic acid. The most abundant KEGG pathway in our results was "Biosynthesis of antibiotics". Our findings support that exogenous application of SA to plantlets induced the activation of PRs, RGAs, ICS2, NLTP2, PER4, TRXM and some WRKYs mediated by NPR1-dependent pathways. Also, we found DEUs, such as BZR1, HSL1, and WHY2 that support that SA could regulate defense-related genes through NPR1-independent pathways. CONCLUSION: The present study of massive data analysis carried out on coconut plantlets exposed to SA, generates valuable information that increases our understanding of defense molecular mechanisms in coconut and open new venues for research for the improvement of management of coconut diseases.
Authors: Wei Fan; He Qiang Lou; Yu Long Gong; Mei Ya Liu; Meng Jie Cao; Yu Liu; Jian Li Yang; Shao Jian Zheng Journal: New Phytol Date: 2015-05-13 Impact factor: 10.151
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