In Mexico, mistletoes have several applications in traditional medicine due to the great variety of compounds with biological activities that have not been characterized to date. The goals of the present study are to analyze the composition of minerals and phytochemical compounds in Mexican mistletoes Phoradendron bollanum and Viscum album subs. austriacum qualitatively and quantitatively, identify the compounds using HPLC-MS, and assess the antimicrobial potential in phytopathogenic microorganism control. Mineral content was evaluated with X-ray fluorescence. Three types of extracts were prepared: ethanol, water, and aqueous 150 mM sodium chloride solution. Characterization was carried out using qualitative tests for phytochemical compound groups, analytical methods for proteins, reducing sugars, total phenol, flavonoids quantification, and HPLC-MS for compound identification. The antimicrobial activity of mistletoe's liquid extracts was evaluated by microplate assay. K and Ca minerals were observed in both mistletoes. A qualitative test demonstrated alkaloids, carbohydrates, saponins, flavonoids, tannins, and quinones. Ethanolic extract showed flavonoids, 3845 ± 69 and 3067 ± 17.2 mg QE/g for Phoradendron bollanum and Viscum album subs. austriacum, respectively, while aqueous extracts showed a total phenol content of 65 ± 6.9 and 90 ± 1.19 mg GAE/g Phoradendron bollanum and Viscum album subs. austriacum, respectively. HPLC-MS identified largely hydroxycinnamic acids and methoxycinnamic acids. Clavibacter michiganenses was successfully inhibited by aqueous extract of both mistletoes.
In Mexico, mistletoes have several applications in traditional medicine due to the great variety of compounds with biological activities that have not been characterized to date. The goals of the present study are to analyze the compon class="Chemical">sitionpan> of minpan>erals anpan>d phytochemical compounpan>ds inpan> Mexicanpan> mistletoes pan> class="Species">Phoradendron bollanum and Viscum album subs. austriacum qualitatively and quantitatively, identify the compounds using HPLC-MS, and assess the antimicrobial potential in phytopathogenic microorganism control. Mineral content was evaluated with X-ray fluorescence. Three types of extracts were prepared: ethanol, water, and aqueous 150 mM sodium chloride solution. Characterization was carried out using qualitative tests for phytochemical compound groups, analytical methods for proteins, reducing sugars, total phenol, flavonoids quantification, and HPLC-MS for compound identification. The antimicrobial activity of mistletoe's liquid extracts was evaluated by microplate assay. K and Ca minerals were observed in both mistletoes. A qualitative test demonstrated alkaloids, carbohydrates, saponins, flavonoids, tannins, and quinones. Ethanolic extract showed flavonoids, 3845 ± 69 and 3067 ± 17.2 mg QE/g for Phoradendronbollanum and Viscum album subs. austriacum, respectively, while aqueous extracts showed a total phenol content of 65 ± 6.9 and 90 ± 1.19 mgGAE/g Phoradendronbollanum and Viscum album subs. austriacum, respectively. HPLC-MS identified largely hydroxycinnamic acids and methoxycinnamic acids. Clavibacter michiganenses was successfully inhibited by aqueous extract of both mistletoes.
Entities:
Keywords:
Phoradendron bollanum; Viscum album subs. austriacum; antimicrobial activity; biochemical characterization; mineral characterization; mistletoe
Authors: Elsnoussi Ali Hussin Mohamed; Mun Fei Yam; Lee Fung Ang; Ali Jimale Mohamed; Mohd Zaini Asmawi Journal: J Acupunct Meridian Stud Date: 2013-02-06
Authors: Juan A Ascacio-Valdés; Antonio F Aguilera-Carbó; José J Buenrostro; Arely Prado-Barragán; Raúl Rodríguez-Herrera; Cristóbal N Aguilar Journal: J Basic Microbiol Date: 2016-02-25 Impact factor: 2.281
Authors: D Hernanz; V Nuñez; A I Sancho; C B Faulds; G Williamson; B Bartolomé; C Gómez-Cordovés Journal: J Agric Food Chem Date: 2001-10 Impact factor: 5.279
Authors: U K Ilyas; Muhammed Elayadeth-Meethal; Mohamed Saheer Kuruniyan; Syed Altafuddin Quadri; R S Rajasree; Punnoth Poonkuzhi Naseef Journal: Saudi J Biol Sci Date: 2021-11-22 Impact factor: 4.219
Authors: João V D C Batista; Ana Paula S Matos; Adriana P Oliveria; Eduardo Ricci Júnior; Zaida M Freitas; Catarina A Oliveira; Helena K Toma; Marcia A M Capella; Leandro M Rocha; Ulrike Weissenstein; Stephan Baumgartner; Carla Holandino Journal: Pharmaceutics Date: 2021-12-24 Impact factor: 6.321